<i>Hpa</i>II PCR‐RFLP within a Bov‐A2 element in the promoter of the bovine <i>CYP21</i> (steroid 21‐hydroxylase) gene

Damiani, Giuseppe; Florio, Sofia; Budelli, E.; Bolla, P.; Caroli, Anna

doi:10.1046/j.1365-2052.2000.00609.x

Cited by 7 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results of the Bonferroni test were found to be significant (5%), and each animal that received the C allele tended to weigh on average 10.2 kg less than its contemporary. Several studies have investigated allelic profiles by SNP genotyping in cattle populations (Damiani et al, 2000;Flisikowski et al, 2005;de Souza et al, 2007;Andréa et al, 2007;Biase et al, 2007;Curi et al, 2009;Bagnicka et al, 2010;Souza et al, 2010). Up to 2009, around 500 bulls from 123 farms participating in the Genetic Improvement Program of the Nellore Breed in Brazil have been evaluated for the future multiplication of "positive animals" in commercial herds by artificial insemination, and of this total number, 25% were evaluated in the present study.…”

Section: Resultsmentioning

confidence: 99%

“…The fact that the sample used in this study was composed of animals under progeny test and the lower frequencies of the C allele in the population may have influenced these results. The RFLP/HpaII polymorphism present in the Bov-A2 element (promoter region of the CYP21 gene) is involved in the synthesis of steroid hormones in humans, and its mutation is associated with congenital adrenal hyperplasia (Damiani et al, 2000). This disease is caused by defects of any of the six enzymes that are required for the synthesis of cortisol and aldosterone from cholesterol in the adrenal cortex (Lee, 2001).…”

Section: Resultsmentioning

confidence: 99%

“…The RFLP/HpaII polymorphism is in the Bov-A2 element (considered to be a short interspersed nucleotide element, SINE) present in the promoter region of the CYP21 gene (steroid 21-hydroxylase) on bovine chromosome 23. In humans, this gene is involved in the synthesis of steroid hormones, and its mutation is associated with congenital adrenal hyperplasia (Damiani et al, 2000). Thus, the aim of the present study was to identify two SNPs in Nellore cattle and connect aspects of the genotype that may be associated with some expected progeny differences for reproductive traits and growth in these animals.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Association between IGF2 and CYP21 gene polymorphisms and characteristics of economic interest in Nellore cattle

Martins-da-Silva¹,

Rios²,

Ramos³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. We analyzed two single nucleotide polymorphisms (SNPs) of the IGF2 and CYP21 genes in Nellore cattle participating in the Brazilian Animal Breeding Program. The SNPs were found in exon 6 of the IGF2 (insulin-like growth factor 2) gene (RFLP/MboII) as well as in the promoter region of the CYP21 (steroid 21-hydroxylase) gene (RFLP/HpaII) of these animals. The TC heterozygotes were significantly more frequent than CC and TT homozygotes in the RFLP/ MboII polymorphism. The T allele was significantly more frequent than the C allele in RFLP/HpaII polymorphism. This population was found IGF2 and CYP21 gene polymorphisms in Nellore cattle to be in Hardy-Weinberg equilibrium for these SNPs. Association of these polymorphisms with expected progeny differences of reproductive and productive traits was investigated, but proved to be significant only for DP550 (expected progeny differenced for weight at 365 days -IGF2 -RFLP/MboII) and DP450 (expected progeny differenced for weight at 450 days -CYP21 -RFLP/HpaII). This is the first study on the occurrence of these two polymorphisms in this Zebu breed of cattle. A total of 147 Nellore animals participating in the Breeding Program of the Nellore Breed (PMGRN) under the management of the National Association of Breeders and Researchers (ANCP) in the city of Ribeirão Preto were analyzed.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Association between IGF2 and CYP21 gene polymorphisms and characteristics of economic interest in Nellore cattle

Martins-da-Silva¹,

Rios²,

Ramos³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The Bov-A2 element is composed of two monomers, called Bov-A units (a segment of 115 bp), joined by a linker sequence of short repeats (CACTTT)n, with approximately 220,000 copies in the entire genome (Onami et al, 2007). Damiani et al (2000a) showed that the mutation frequency (substitutions and deletions/insertions per nucleotide site per year) of the Bov-A2 sequences is generally higher than those of other genomic noncoding sequences. This high amount of genetic variation in the Bov-A2 elements could be responsible for several processes such as recombination and gene conversion.…”

Section: Introductionmentioning

confidence: 99%

Short Communication Identification of a DNA methylation point in the promoter region of the bovine CYP21 gene

Silva

Freitas

Rios³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The CYP21 (steroid 21-hydroxylase) gene is involved in the synthesis of steroid hormones. Bov-A2 is a retroposon that is common in ruminant genomes. The promoter region of bovine CYP21 contains a short interspersed nucleotide element of Bov-A2, which overlaps a putative Sp1 binding site. We looked for RFLP/HpaII polymorphism in the Bov-A2 element in bovine Zebu breeds by PCR-RFLP, and examined whether polymorphism in this element is associated with methylation. Among DNA samples from 135 Brazilian Zebu breed cattle, we identified an RFLP/HpaII polymorphism (T/C), which, based on a restriction methylation-sensitive assay employing HpaII and isoschizomer MspI enzymes (methylation-sensitive and -non-sensitive enzymes, respectively), appears to be a DNA methylation point. This is the first report of this polymorphism and on DNA methylation in the bovine CYP21 promoter region in Brazilian Zebu cattle.

show abstract

“…These specific retroelements may be very important, therefore, in the present study, polymorphic site (GenBank no. M11267) in the promoter region, recognized by the HpaII exonuclease (Damiani et al 2000a) was chosen for analysis.…”

mentioning

confidence: 99%

An analysis of CYP19, CYP21 and ER genotypes in Polish Holstein-Friesian cows with regard to the selected reproductive traits

et al. 2011

View full text Add to dashboard Cite

The aim of this study was to relate polymorphic variants of CYP19, CYP21 and ER1 genes to reproductive traits in 472 Polish Holstein-Friesian cows. High frequencies of one of the homozygous genotypes were found. The ER1/SnaBI AA homozygotes were not identified. In the first and third lactation, an average calving-to-conception interval (CLVC) in cows of ER1/SnaBI GG genotype was significantly shorter (P ≤ 0.05) than in heterozygous cows. In the cows of ER1/ BglI GG genotype, significantly shorter CLVC (P ≤ 0.05) was observed compared to heterozygotes in the first lactation, whereas in the third lactation, CLVC in homozygous cows was significantly longer (P ≤ 0.05) than in heterozygous ones. It was also found that homozygous cows were characterized by significantly longer calving interval (CLVI; P ≤ 0.05) compared to heterozygotes in the third lactation. Longer CLVCs in CYP19 AA cows were found, compared to heterozygotes, and this difference was significant in the first and third lactation (P ≤ 0.05). Similarly, the average CLVIs were longer in CYP19 AA homozygotes than in heterozygous cows; however, significance was proven only in the third lactation (P ≤ 0.05). Description of the molecular mechanisms regulating reproduction, and thus identification of the individuals of genotypes with optimal potential may facilitate the employment of selected reproductive model by a breeder. Gene polymorphism, calving interval, calving-to-conception interval, pregnancy length, inseminationsAbbreviations: CYP19 -cytochrome P450 aromatase gene, CYP21 -steroid 21-hydroxylase gene, ER -oestrogen receptor gene, CLVC -calving-to-conception interval, CLVI -calving interval, INSEM -number of services per successful conception, PREG -pregnancy length.Genetic improvement of the reproductive traits in dairy cattle is crucial not only for ensuring quantitative aspect of production but mainly for the efficient course of lactogenesis and lactopoesis. It is an extremely difficult process, regulated at many stages in a multifactorial manner. Moreover, coefficients characterizing reproductive traits are of low heritability and there is a small number of described genetic markers associated with reproduction that could be used as potential factors supporting the classical selection methods. Therefore, searching for such markers is admittedly important both for the application and research purposes. So, the analysis of the influence of different polymorphic variants of enzymes crucial for oestrogen synthesis on the reproductive traits or of protein factors mediating their actions seems fully justified.The reproductive cycle of all mammalian females is regulated mainly by oestrogen hormones. They are synthesized through the aromatization of androgens, which, in females, are mainly of ovarian origin. In this complex process, a key role is played by the p450 aromatase, which is an enzymatic complex comprising two proteinsnonspecific microsomal flavoprotein reductase and specific haemoglycoprotein, that is, cytochrome P450 aromatase, coded f...

show abstract

HpaII PCR‐RFLP within a Bov‐A2 element in the promoter of the bovine CYP21 (steroid 21‐hydroxylase) gene

Cited by 7 publications

References 3 publications

Association between IGF2 and CYP21 gene polymorphisms and characteristics of economic interest in Nellore cattle

Association between IGF2 and CYP21 gene polymorphisms and characteristics of economic interest in Nellore cattle

Short Communication Identification of a DNA methylation point in the promoter region of the bovine CYP21 gene

An analysis of CYP19, CYP21 and ER genotypes in Polish Holstein-Friesian cows with regard to the selected reproductive traits

Contact Info

Product

Resources

About