In situcrosslinkable elastomeric hydrogel for long-term cell encapsulation for cardiac applications

Abstract

The regenerative therapy of tissues relays on successful cell transplantation and engraftment. Soft hydrogel carriers are employed to protect transplanted cells from harmful microenvironment in soft tissue regeneration. Herein an injectable, porous, biodegradable, bioresorbable, and elastomeric hydrogel fabricated from poly(propylene fumarate-co-sebacate-co-ethylene glycol) crosslinked with PEGDA for cardiomyoblast encapsulation was reported. The hydrogel retains adequate mechanical property in the range of native myocardium even after 30 days of degradation (49 ± 0.008 kPa). The hydrogel shows maximum extensibility without collapsing even under 60% compression. The hydrogel retains 70.58% equilibrium water content, wide internal porosity, and slow bulk degradation favorable for cell carriers. The cardiomyoblast cells encapsulated in hydrogel retains viability even after 30 days of culture. The long-term viability and proliferation studies of encapsulated cells in the hydrogel substantiate the suitability of hydrogel microenvironment for cell survival. The present hydrogel is a potential cell carrier with favorable physical and biological properties for cell encapsulation for cardiac applications. The candidate hydrogels perform better than the other reported elastomeric hydrogels fabricated for cell therapy. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2936-2944, 2016.

Nontoxic Initiator Alternatives to TEMED for Redox Hydrogel Polymerization

“…The initiator system candidates we screened were fabricated relative to the control TEMED gel (41 mM, 1 NR x ). This initial concentration was determined by averaging previously reported TEMED concentrations in the literature. − The concentration of APS was kept constant at 25 mM under all conditions. Each TEMED molecule displays two amine groups; therefore, the TEMED 1 NR x control gel, containing 41 mM TEMED, contains twice the amount of amine groups (82 mM).…”

Fumarate‐co‐PEG‐co‐sebacate photopolymer and its evaluation as a drug release system

“…Thus, reactions with higher FA ratios required longer reaction times. It is worth to mention that, in clear contrast with the method published for synthesis of linear poly(fumarate-co-PEG-co-sebacate) s, 32,33 metallic catalysts were not required herein. Furthermore, the use of TsOH simplifies the purification of polymers and post-process operations since de catalyst is easily removed during the washing steps with ethanol/ water mixtures.…”

“…The properties and the behavior of these materials as elution systems for Cephalexin and Progesterone, as model hydrophilic and hydrophobic drugs, are also explored. It should be mentioned that although some few examples of fumarate-co-PEG-co-sebacate copolymers have been published, [31][32][33] these precursors were only employed to obtain hydrogels using high MW PEGs, and that their use for the preparation of films by light triggered reactions at the level of FA groups have so far not been explored.…”

Injectable Hydrogel-Based Nanocomposites for Cardiovascular Diseases

“…One of the aspects of current research on injectable hydrogels for transporting cells is to design a hydrogel that is more compatible with cells (Lovett et al, 2009). A polyethylene glycol (PEG) PEGylated fibrin proposed by Geuss et al (2015) and an injectable hydrogel combained poly (propylene fumarate-co-sebacate-coethylene glycol) with PEGDA designed by Komeri and Muthu (2016) are also suitable for cardiomyocytes. In addition, hydrogel for CVDs should be electrically conductive to generate electrical signals to the myocardium (Sepantafar et al, 2016).…”