<i>In situ</i> distribution of transforming growth factor α in normal human tissues and in malignant tumours of the ovary

Kommoss, Friedrich; Wintzer, H. O.; Kleist, S. von; Kohler, Manuela; Walker, Rosemary A.; Langton, Beatrice C.; Tran, Kai; Pfleiderer, Albrecht; Bauknecht, Thomas

doi:10.1002/path.1711620308

Cited by 51 publications

(20 citation statements)

References 34 publications

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“…We can discuss our data qualitatively or quantitatively. Qualitatively, our detection of TGF-· in the sera of controls and ovarian cancer patients is consistent with the radioimmunoassay (RIA) results of Owens and Leake [16] who found TGF-· present in the tissue of approximately 84% of normal ovaries, and with the follow-up study of Owens et al [17] and with Kommoss et al [18] who performed immunohistochemistry on frozen tumor tissue. Only 6 of our 36 ascites samples had detectable TGF-· levels.…”

Section: Discussionsupporting

confidence: 76%

Transforming Growth Factor-Alpha Levels in the Serum and Ascites of Patients withAdv anced Epithelial Ovarian Cancer

Saltzman¹,

Hartenbach²,

Carter³

et al. 1999

Gynecol Obstet Invest

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A variety of cytokines have been identified to play a role in ovarian cancer. In this pilot study, we sought to determine whether transforming growth factor-α (TGF-α) was detectable in the serum and ascites of women with advanced stage epithelial ovarian cancer. TGF-α was measured using an enzyme-linked immunosorbent assay and was present in 18 of 25 control sera. Prior to treatment for stage III or IV epithelial ovarian cancer, 18 patients had undetectable serum levels of TGF-α, while 18 had values ranging from 10.6 to 531.7 pg/ml. The group with undetectable levels had a 6-month greater median survival; detectable TGF-α might be a negative prognostic indicator. In a separate group undergoing second-look laparotomy, differences in median TGF-α values versus controls and the primary study group approached significance. TGF-α was detected in significantly more control peritoneal fluid samples than in patient ascites. A larger study is warranted.

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Section: Discussionsupporting

confidence: 76%

Transforming Growth Factor-Alpha Levels in the Serum and Ascites of Patients withAdv anced Epithelial Ovarian Cancer

Saltzman¹,

Hartenbach²,

Carter³

et al. 1999

Gynecol Obstet Invest

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“…Although these authors observed EGF and TGF-a in both epithelial and extraepithelial (such as muscular layer) tissues, we failed to demonstrate the expression of these proteins in extraepithelial tissues. Our result was consistent with a previous report [21] that TGF-a was localized to epithelial cells in the human fallo pian tube.…”

Section: Discussionsupporting

confidence: 83%

Expression of Epidermal Growth Factor and Transforming Growth Factor-Alpha in Fallopian Tube Epithelium and Their Role in Embryogenesis

Kurachi

Morishige²,

Imai³

et al. 1994

Horm Res

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We studied the expression of epidermal growth factor (EGF) and transforming growth factor (TGF)-α in human fallopian tube epithelium at various menstrual stages. Immunohistochemical staining using anti-EGF and anti-TGF-α antibodies showed a specific staining in ampullary tube epithelium at late follicular and luteal stages but the staining was very weak at the early follicular stage. Quantitative reverse transcription and polymerase chain reaction (RT-PCR) using β-actin mRNA as an internal standard revealed the menstrual-stage-specific expression of EGF and TGF-α gene transcripts: amounts of EGF and TGF-α mRNA relative to those of β-actin were significantly higher at late follicular and luteal stages than at the early follicular stage. To clarify the biological role of these growth factors, mouse 2-cell embryos were cocultured with human fallopian tube epithelial cells with or without blocking the action of these growth factors. Cocultures significantly promoted blastocyst formation, but this promotive effect of the tubal epithelial cells was completely abolished by the addition of anti-EGF and/or anti-TGF-α monoclonal neutralizing antibodies to the coculture system. These results demonstrated that EGF and TGF-α were synthesized and expressed in fallopian tube epithelium at specific menstrual stages, and may be involved in early embryonic development.

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“…there is a significant correlation between the presence of mRNA in neoplastic tissue and the expression of the respective peptide [56,57], While it has been reported that EGF mRNA is present in only 33% of gastric carci nomas. abundant TGF-a mRNA and EGF-R mRNA is found in all gastric carcinoma cell lines [36, 58.…”

Section: Expression Of Egf Tgf-a and Egf-r In Gastric Adenocarcinomasmentioning

confidence: 93%

Expression of Epidermal Growth Factor, Transforming Growth Factor Alpha and Their Receptor in Gastro-Oesophageal Diseases

Jankowski

Hopwood

Wormsley³

1993

Dig Dis

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This article is a review of aspects of the expression of the regulatory peptides; epidermal growth factor (EGF), transforming growth factor alpha (TGF-α), and their receptor (EGF-R) in the epithelium of the human oesophagus and stomach in health and disease. It has become clear that TGF-α has increased expression in metaplastic, dysplastic and neoplastic tissue of the oesophagus compared with normal mucosa. The degree of abnormal expression becomes more marked as dysplasia increases. TGF-α expression is also increased in gastric neoplasias. EGF has a different pattern of expression, being decreased in oesophagitis and increased in gastritis. Although EGF is present in Barrett’s oesophagitis, the expression of EGF does not discriminate between dysplastic and neoplastic epithelium. EGF-R is normally expressed on all gastrointestinal epithelia, but its expression is increased in Barrett’s epithelium, as well as in adenocarcinomas of the oesophagus and the stomach. The two peptides bind to their receptors on the mucosal cell membranes, and the co-expression of peptide and receptor is positively associated with varying degrees of cellular proliferation. The density of receptor expression may modulate the proliferative stimulus, leading to either mitogenic (regulated) or oncogenic (unregulated) growth.

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In situ distribution of transforming growth factor α in normal human tissues and in malignant tumours of the ovary

Cited by 51 publications

References 34 publications

Transforming Growth Factor-Alpha Levels in the Serum and Ascites of Patients withAdv anced Epithelial Ovarian Cancer

Transforming Growth Factor-Alpha Levels in the Serum and Ascites of Patients withAdv anced Epithelial Ovarian Cancer

Expression of Epidermal Growth Factor and Transforming Growth Factor-Alpha in Fallopian Tube Epithelium and Their Role in Embryogenesis

Expression of Epidermal Growth Factor, Transforming Growth Factor Alpha and Their Receptor in Gastro-Oesophageal Diseases

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