<i>In-situ</i> implant containing PCL-curcumin nanoparticles developed using design of experiments

Kasinathan, Narayanan; Amirthalingam, Muthukumar; Reddy, Neetinkumar D.; Jagani, Hitesh; Volety, Subrahmanyam Mallikarjuna; Rao, J. Venkata

doi:10.3109/10717544.2014.927021

Cited by 20 publications

(23 citation statements)

References 16 publications

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“…However during preclinical and clinical studies it would be easier to control pharmaceutical properties such as drug release and site specificity if hyaluronidase is given using a carrier system [ 5 ]. Among the various polymers used as drug carriers poly(lactic-co-glycolic) acid (PLGA) is widely used due to its biocompatibility [ 6 ]. Hyaluronidase loaded PLGA when suitably modified would help in targeting cancerous cells.…”

Section: Introductionmentioning

confidence: 99%

“…Among the various carriers used in the preparation of nanoparticles, PLGA is one of the safest polymers with good biodegradable properties whose degradation time can be altered by altering the percentage of lactic acid. This provides a wide option for the researcher to select the appropriate PLGA fraction based on the requirement [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…In any experiment it is important to understand the process engineering involved in drug formulations to achieve consistent product attributes [ 10 ]. When compared to the one-factor-at-a-time (OFAT) design, design of experiments (DoE) allows in-depth analysis of process engineering involved in drug formulations [ 11 ]. In OFAT design, the effect of process variables on the outcome is studied by varying only one variable at a particular time.…”

Section: Introductionmentioning

confidence: 99%

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A Fractional Factorial Design to Study the Effect of Process Variables on the Preparation of Hyaluronidase Loaded PLGA Nanoparticles

2014

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The present study was initiated to understand the effect of PLGA concentration, PVA concentration, internal-external phase ratio, homogenization speed, and homogenization time on mean particle size, zeta potential, and percentage drug encapsulation using fractional factorial design. Using PLGA (50-50) as the carrier, hyaluronidase loaded PLGA nanoparticles were prepared using double emulsion solvent evaporation technique. The particle size was analyzed by dynamic light scattering technique and protein content by Lowry method. The study showed that homogenization speed as an independent variable had maximum effect on particle size and zeta potential. Internal-external phase volume ratio had maximum effect on drug encapsulation. Mean particle size also had high dependency on the combined effect of PVA concentration and phase volume ratio. Using fractional factorial design particle size of <400 nm, zeta potential of <−30 mV, and percentage encapsulation of 15–18% were achieved.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Fractional Factorial Design to Study the Effect of Process Variables on the Preparation of Hyaluronidase Loaded PLGA Nanoparticles

2014

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“…Design of experiments (DoE) is one of the mathematical models available for systematic evaluation of variables involved in any process (Guideline 2009). DoE represents a multivariate approach in identifying the conditions for achieving a product with the highest quality attributes and less batch-to-batch variations (Kasinathan et al 2014a). Although DoE has its own pitfalls, it helps in mining and analyzing complex information in real time, besides improving the understanding of the process in a short time (Ferreira and Tobyn 2014).…”

Section: Introductionmentioning

confidence: 99%

Polycaprolactone-based in situ implant containing curcumin-PLGA nanoparticles prepared using the multivariate technique

Kasinathan

Amirthalingam

Reddy

et al. 2015

Artificial Cells, Nanomedicine, and Biotechnology

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Studies on the effect of curcumin/PLGA ratio (CPR), stabilizer (PVA) concentration, homogenization speed, homogenization time, and sonication time on mean particle size (MPS) and percentage drug encapsulation (PDE) were performed using the multivariate technique. MPS and PDE were found to be more dependent on the interaction of sonication time with the other variables. Curcumin was released in a sustained manner from curcumin-PLGA nanoparticles (CPN). CPN improved the survival rate of Ehrlich ascites carcinoma (EAC)-bearing mice and controlled the EAC-induced change in hematological parameters. Histopathology of vital organs showed that the formulation was safe. Polycaprolactone was used in preparing an in situ implant containing CPN.

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“…Kasinathan et al studied the effect of processing parameters on the curcumin‐loaded PCL nanoparticles. The results showed that the homogenization speed and concentration of second emulsion showed high influence when compared to other parameters …”

Section: Introductionmentioning

confidence: 99%

Fabrication of chitosan/collagen/hydroxyapatite scaffolds with encapsulated Cissus quadrangularis extract

Thongtham

Chai‐in

Unger

et al. 2020

Polymers for Advanced Techs

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Here, we demonstrated the fabrication of a composite scaffold (chitosan [CS], collagen [Col], and hydroxyapatite [HA]) with the incorporation of encapsulated Cissus quadrangularis (CQ) extract for tissue engineering applications. First, the crude extract of CQ loaded nanoparticles were synthesized via double emulsion technique using polycaprolactone (PCL) and polyvinyl alcohol (PVA) as oil and aqueous phases, respectively. Both PCL (20, 40, and 80 mg/mL) and PVA (0.5%, 1%, and 3% w/v) concentrations were varied to determine the optimum concentrations for CQ-loaded nanoparticle preparation. The CQ-loaded PCL nanoparticles (CQ-PCL NPs), prepared with 20 mg/mL PCL and 0.5% (w/v) PVA, exhibited the smallest size of 334.22 ± 43.21 nm with 95.54 ± 1.49% encapsulation efficiency. Then, the CQ-PCL NPs were incorporated into the CS/Col/HA scaffolds. These scaffolds were also studied for their ultrastructure, pore sizes, chemical composition, compressive modulus, water swelling, weight loss, and biocompatibility. The results showed that the addition of CQ-PCL NPs into the scaffolds did not dramatically alter the ultrastructure and properties of the scaffolds, compared to CS/Col/HA scaffolds alone. However, incorporation of CQ-PCL NPs in the scaffolds improved the release profile of CQ by preventing the initial burst release and prolonging the release rate of CQ. In addition, the CQ-PCL NPs-loaded CS/Col/HA scaffolds supported the attachment and proliferation of MC3T3-E1 osteoblast cells.

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In-situ implant containing PCL-curcumin nanoparticles developed using design of experiments

Abstract: This implant could be further evaluated for pharmacological activities.

Cited by 20 publications

References 16 publications

A Fractional Factorial Design to Study the Effect of Process Variables on the Preparation of Hyaluronidase Loaded PLGA Nanoparticles

A Fractional Factorial Design to Study the Effect of Process Variables on the Preparation of Hyaluronidase Loaded PLGA Nanoparticles

Polycaprolactone-based in situ implant containing curcumin-PLGA nanoparticles prepared using the multivariate technique

Fabrication of chitosan/collagen/hydroxyapatite scaffolds with encapsulated Cissus quadrangularis extract

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