<i>In situ</i> measurement of leaf chlorophyll concentration: analysis of the optical/absolute relationship

Parry, Christopher K.; Blonquist, J. M.; Bugbee, Bruce

doi:10.1111/pce.12324

Cited by 206 publications

(187 citation statements)

References 52 publications

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“…Similarly to WI, the plant chlorophyll status reduction caused by water stress promoted decrease of the CCI values. However, this decline followed a nonlinear and slightly convex trend, as reported in previous scientific articles [27][28][29][30]. Past scientific studies also evaluated plant water status from WI for distinct vegetation species under drought conditions (Table 1), agreeing with the variation range obtained for sunflower plants in this study.…”

Section: Resultssupporting

confidence: 90%

“…Similarly to WI, the plant chlorophyll status reduction caused by water stress promoted decrease of the CCI values. However, this decline followed a nonlinear and slightly convex trend, as reported in previous scientific articles [27][28][29][30]. De Maria et al [31] evaluated the effects of soil cadmium contamination on accumulation and distribution, growth and physiological responses of sunflower plants, obtaining total chlorophyll contents varying from 0.022 to 0.034 mg•cm −2 .…”

Section: Resultsmentioning

confidence: 69%

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Assessment of Photosynthetic Pigment and Water Contents in Intact Sunflower Plants from Spectral Indices

2017

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Under water-limited conditions, monitoring water and chlorophyll status is essential to avoid restrictions in crop growth and yield. This study was carried out to assess water and chlorophyll contents from spectral indices in sunflower plants. The hybrid Sunbright Supreme was cultivated inside a non-acclimatized greenhouse until the start of the flowering stage, and later was maintained in a growth chamber with the purpose of submitting the plants to a slow and progressive dehydration rate for 12 consecutive days. Spectral (reflectance and transmittance), leaf masses (fresh and dry), and total chlorophyll measurements were accomplished in sunflower plants. The water stress caused a reduction in the water and chlorophyll contents, resulting in linear and nonlinear decreases for the spectral indicators Water Index (WI) and Chlorophyll Content Index (CCI), respectively. The low scattering of the average values around the fitted models indicates that WI and CCI were effective in representing changes in water and chlorophyll status for sunflowers (R 2 = 0.912 and R 2 = 0.905). The benefits of using hand-held optical meters for reflectance and transmittance are that they enable rapid, accurate, and nondestructive assessments of water and chlorophyll contents in sunflower plants from radiometric indicators.

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Section: Resultssupporting

confidence: 90%

“…Similarly to WI, the plant chlorophyll status reduction caused by water stress promoted decrease of the CCI values. However, this decline followed a nonlinear and slightly convex trend, as reported in previous scientific articles [27][28][29][30]. De Maria et al [31] evaluated the effects of soil cadmium contamination on accumulation and distribution, growth and physiological responses of sunflower plants, obtaining total chlorophyll contents varying from 0.022 to 0.034 mg•cm −2 .…”

Section: Resultsmentioning

confidence: 69%

Assessment of Photosynthetic Pigment and Water Contents in Intact Sunflower Plants from Spectral Indices

2017

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“…For the second step, there was a good linear relationship between the observed LCC value and leaf chlorophyll contents, but there was a better relationship compared with chlorophyll meter data. Some of the variation between chlorophyll meter values and chlorophyll contents comes from differences in methodology (Parry et al, 2014), and some of the variation is from differences in leaf structure (Sims and Gamon, 2002;Hunt and Daughtry, 2014). Various crops or cultivars have different relationships between LCC panel values and chlorophyll contents (Haripriya Anand and Byju, 2008).…”

Section: Genetics ´ Environment ´ Managementmentioning

confidence: 99%

Assessment of Leaf Color Chart Observations for Estimating Maize Chlorophyll Content by Analysis of Digital Photographs

Friedman

Hunt

Mutters³

2016

Agronomy Journal

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Developed as a nondestructive aid for estimating the N content in rice (Oryza sativa L.) crops, leaf color charts (LCCs) are a numbered series of plastic panels that range from yellow‐green to dark green. By visual comparison, the panel value closest in color to a leaf indicates whether N is deficient, sufficient, or in excess. Because the selected values depend on subjective decisions by an observer, our goal was to determine whether spectral reflectances or digital color photographs could provide an objective, reproducible, and potentially automated method for determining LCC values. Maize (Zea mays L.) leaves were collected on two dates from an ongoing N fertilization experiment. Observed LCC panel values of selected leaves were highly correlated to chlorophyll content and chlorophyll meter values. Spectral reflectances and digital photographs were analyzed to predict the LCC panel value that was observed for each leaf. Supervised classifications of digital photographs using minimum distance provided reasonable predictions of the LCC value, but the spectral angle mapper did not. The dark green color index and the triangular greenness index predicted LCC panel values well. Uncorrected digital photographs (raw) produced better agreement with observed LCC panel values when using spectral indices, whereas color‐corrected photographs (JPEG) produced better agreement using the supervised classification methods. We concluded that subjective visual observations using a LCC were not worse than the objective methods for estimating leaf chlorophyll content. Visual observations using LCCs may be an easy‐to‐use and low‐cost method for managing N at smaller scales.

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“…Para tanto, utilizou-se um clorofilômetro portátil (modelo CCM-200, OptiScience) (Gonçalves et al 2008;Parry et al 2014).…”

Section: Conteúdo De Clorofilasunclassified

Fotossíntese de espécies de Anibae em resposta à exposição a ambientes contrastantes de luz

et al. 2018

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ResumoFotossíntese de espécies de Anibae em resposta à exposição a ambientes contrastantes de luz. Características ecofisiológicas e o ambiente de luz têm implicações sobre o estabelecimento de plantios florestais. O objetivo desse estudo foi investigar a fotossíntese de Aniba canelilla e A. rosaeodora em resposta à disponibilidade de luz no campo. As trocas gasosas, conteúdo de clorofilas (ICC) e desempenho fotoquímico (F V /F M ) foram medidos em folhas aclimatadas (sombra durante 300 dias), a pleno sol (28 dias) e novamente à sombra durante 28 dias. A fotossíntese, ICC e F V /F M de A. canelilla e A. rosaeodora foram afetadas negativamente a pleno sol, mas se recuperaram quando submetidas à sombra. A fotossíntese, ICC e F V /F M foram duas vezes superiores nas plantas de sombra. Aniba rosaeodora apresentou desempenho fotossintético duas vezes superior a A. canelilla. As diferenças nas respostas fotossintéticas à luz sugerem que as espécies poderiam ocupar "nichos" de sucessão diferentes em plantios de enriquecimento e, a plasticidade (mediada pela recuperação) implica aclimatação em ambientes de luz variável. O fato das espécies de Anibae estudadas possuírem diferenças de plasticidade para luz deve ser visto com cautela para sugerir plantios convencionais, mas reforça a indicação para plantios de enriquecimento em sítios florestais com variações na disponibilidade de luz. Palavras-chave: Aniba canelilla, Aniba rosaeodora, fluorescência da clorofila a, plantios florestais, trocas gasosas. AbstractPhotosynthesis of species of Anibae in response to the exposure to contrasting light environments. Ecophysiological traits and the light environment have implications for the establishment of forest plantations. The aim of this study was to investigate the photosynthetic activity of Aniba canelilla and A. rosaeodora in response to changes in the availability of light in the field. Gas exchange, content of chlorophylls (ICC) and photochemical performance (F V /F M ) were measured in leaves acclimated in the shade during 300 days, after in the full sun (28 days) and again in the shade by 28 days. The photosynthesis, ICC and F V /F M of A. canelilla and A. rosaeodora were negatively affected in full sunlight, but the plants recovered when submitted to shade. Photosynthesis, ICC and F V /F M were twice as high in shade plants. Aniba rosaeodora presented photosynthetic performance twice higher than A. canelilla. Differences in photosynthetic responses to light suggest that species could occupy different succession "niches" in enrichment plantation, and plasticity (recovery-mediated) implies acclimatization under variable light environments. The fact that the studied species of Anibae have differences in plasticity for light should be viewed with caution to suggest conventional plantations, but it reinforce the indication for enrichment plantations in forest sites with variations in the availability of light.

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In situ measurement of leaf chlorophyll concentration: analysis of the optical/absolute relationship

Cited by 206 publications

References 52 publications

Assessment of Photosynthetic Pigment and Water Contents in Intact Sunflower Plants from Spectral Indices

Assessment of Photosynthetic Pigment and Water Contents in Intact Sunflower Plants from Spectral Indices

Assessment of Leaf Color Chart Observations for Estimating Maize Chlorophyll Content by Analysis of Digital Photographs

Fotossíntese de espécies de Anibae em resposta à exposição a ambientes contrastantes de luz

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