In situ mineralized PLGA/zwitterionic hydrogel composite scaffold enables high-efficiency rhBMP-2 release for critical-sized bone healing

Abstract

Seeking an osteoconductive and osteoinductive scaffold is highly desirable for functional restoration of large bone defects. Here, we report an in situ mineralized poly(lactic-co-glycolic acid)/poly[2-(methacryloyloxy)ethyl] dimethyl-(3-sulfopropyl)ammonium hydroxide hydrogel (PLGA/PSBMA) scaffolds...

Biomaterials for bone defect repair: Types, mechanisms and effects

“…Polysaccharides, proteins, bioceramics, bioactive proteins, metal ions and nanomaterials Wang et al, 72 Quinlan et al, 76 Liu et al, 78 Zhang et al, 79 Xu et al, 81 Lou et al, 83 Xu et al, 84 Ding et al, 89 , Zeng et al, 90 Wei et al, 91 Kaizawa et al, 92 , Tovar et al, 94 Wang et al, 96 Ren et al, 97 and Vig and Fernandes 98 perfect biomaterial scaffold should have good mechanical properties, hydrophilicity and porosity for mutual exchange of body fluids and components, biological properties to promote cell adhesion, proliferation and vascularisation and biodegradable properties. Biomaterial scaffolds play an important role as carriers of biologically active factors, drugs, metal ions and stem cells in terms of their efficiency in delivering and controlling the release of substances.…”

“…77 One study used poly [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (PSBMA) as a hydrogel scaffold to load bioactive proteins to achieve sustained release of ionic proteins. 78 Zhang et al 79 used cysteine-modified carboxymethyl chitosan and oxidised sodium alginate to form a stable monolayer hydrogel network through covalent bonding by Schiff base reaction; Wang et al 72 cross-linked maleic anhydride with chitosan and grafted with L-cysteine via click chemistry to form an amphoteric hydrogel to repair bone defects.…”

Metabolic Response Modulations by Zwitterionic Hydrogels for Achieving Promoted Bone Regeneration

“…The 3D ECM-mimetic zwitterion-mediated osteogenesis preferences are consistent with previous studies that zwitterionic hydrogel promoted HA biomineralization in vitro and in vivo. [20,24,33,34] Matrix stiffness has been commonly recognized to decide stem cell fate. 3D ECM with low stiffness promotes adipogenesis; moderate stiffness favors myogenesis, while high stiffness enhances osteogenesis.…”

“…[24] This scaffold also promoted the repair of critical-sized calvarial defect of rats. [34,35] However, these findings preliminarily reveal the potential of zwitterionic hydrogels in bone tissue engineering, but they only focus on the role of the heterogeneous mineralization process. The systematic understanding about how zwitterionic hydrogels regulate osteogenesis of bone marrow mesenchymal stem cells (BMSCs) and bone regeneration is still limited.…”

Zwitterionic/phosphonate copolymer coatings endow excellent antifouling properties and robust re-mineralization ability of dentine substrates

“…48 In recent years, zwitterionic material based ligands have shown a great potency for dense biomineralization scaffolds due to their unique oppositely charged structures that is able to mimic the positively and negatively charged residues of collagen and non-collagen proteins during the nature biomineralization of bone tissues. 36,49,50 To test the potential ability of the zwitterionic coating to induce the re-mineralization of the dental hard tissues, the re-mineralization of acid-etched dentine sections was systematically investigated both in vitro and in vivo.…”