<i>In vitro</i> and <i>in vivo</i> gene delivery using chitosan/hyaluronic acid nanoparticles: Influences of molecular mass of hyaluronic acid and lyophilization on transfection efficiency

Sato, Toshinori; Nakata, Mitsuhiro; Yang, Zhihong; Torizuka, Yu; Kishimoto, Satoko

doi:10.1002/jgm.2968

Cited by 24 publications

(20 citation statements)

References 36 publications

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“…The toxicity and immunogenicity were relatively low, and these can self-assemble into stable core-shell structures with RNA or DNA to protect RNA or DNA from degradation by serum enzymes. The chitosan nanoparticles delivery system ensures the efficiency of gene delivery and accurate expression (Sato et al., 2017). Based on the above studies of OCT and chitosan, OCT in this study was conjugated to the CS-MB, enabling the CS-MB to better exert the function of recognizing tumor cells.…”

Section: Resultsmentioning

confidence: 99%

Identification and imaging of miR-155 in the early screening of lung cancer by targeted delivery of octreotide-conjugated chitosan-molecular beacon nanoparticles

et al. 2018

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Lung cancer is still the most common cancer globally. Early screening remains the key to improve the prognosis of patients. There is currently a lack of specific and sensitive methods for early screening of lung cancer. In recent years, studies have found that microRNA plays an important role in the occurrence and development of lung cancer and become a biological target in the early diagnosis of lung cancer. In this study, lung cancer cells, subcutaneous xenografts of lung cancer in nude mice, and Lox-Stop-lox K-ras G12D transgenic mice were used as models. The transgenic mice displayed the dynamic processes from normal lung tissue to atypical hyperplasia, adenomas, carcinoma in situ and lung adenocarcinoma. It was found that miR-155 and somatostatin receptor 2 (SSTR2) were expressed in all the disease stages of transgenic mice. Through molecular beacon (MB) technology and nanotechnology, chitosan-molecular beacon (CS-MB) nanoparticles and targeted octreotide (OCT) were conjugated and synthesized. The octreotide-conjugated chitosan-molecular beacon nanoparticles (CS-MB-OCT) can specifically bind to SSTR2 expressed by the lung cancer cells to achieve the goal of identification of lung cancer cells and imaging miR-155 in vivo and in vitro. Fluorescence imaging at different disease stages of lung cancer in Lox-Stop-lox K-ras G12D transgenic mice was performed, and could dynamically monitor the occurrence and development of lung cancer by different fluorescence intensity ranges. The current research, in turn, provides new idea, new method, and new technology for the early screening of lung cancer.

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Section: Resultsmentioning

confidence: 99%

Identification and imaging of miR-155 in the early screening of lung cancer by targeted delivery of octreotide-conjugated chitosan-molecular beacon nanoparticles

et al. 2018

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“…The highest gene expression in mice, the lactate dehydrogenase activity and concentration of inflammatory cytokines in bronchoalveolar lavage fluid comparable to those caused by ultrapure water [172] Transferrin/HA-pDNA/nanostructured lipid carriers in lung cancer gene therapy Low cytotoxicity, enhanced gene transfer ability in vitro and in vivo [169] HA in cochlear gene therapy Facilitated expression in cells lining the scala media, atraumatic and clinically feasible method to introduce transgenes into cochlear cells [173] pDNA/HA/chitosan complexes Augmented stability and cellular transfection ability of the complexes after lyophilization-rehydration [174] Nanoparticles formed by HA, polyethyleneglycol (HA, PEG) and polyethylenimine (HA, PEI) and pDNA…”

Section: Applications Properties Referencesmentioning

confidence: 99%

Hyaluronan as a Prominent Biomolecule with Numerous Applications in Medicine

Valachová

Šoltés

2021

IJMS

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Hyaluronan (HA) is a natural glycosaminoglycan present in many tissues of all vertebrates. HA has various biological functions, which are dependent on its molar mass. High-molar-mass HA has anti-angiogenic, immunosuppressive and anti-inflammatory properties, while low-molar-mass HA has opposite effects. HA has also antioxidative properties, however on the other hand it can be readily degraded by reactive oxygen species. For many years it has been used in treatment of osteoarthritis, cosmetics and in ophthalmology. In the last years there has been a growing interest of HA to also be applied in other fields of medicine such as skin wound healing, tissue engineering, dentistry and gene delivery. In this review we summarize information on modes of HA administration, properties and effects of HA in various fields of medicine including recent progress in the investigation of HA.

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“…Ishihara and colleagues coated the surfaces of pDNA/chitosan complexes with hyaluronic acid (HA) of varying molecular masses. pDNA complexes were lyophilized and subsequently stored under defined conditions . Transfection efficiencies of pDNA/chitosan/HA ternary complexes were dependent on the average size of HA; the coating with high-molecular-weight HA augmented the stability and transfection ability of polyplexes after lyophilization-rehydration.…”

Section: Recent Designsmentioning

confidence: 99%

“…pDNA complexes were lyophilized and subsequently stored under defined conditions. 39 Transfection efficiencies of pDNA/chitosan/HA ternary complexes were dependent on the average size of HA; the coating with high-molecular-weight HA augmented the stability and transfection ability of polyplexes after lyophilization-rehydration. Intratumoral injection of lyophilized, rehydrated polyplexes encoding the thymidine kinase suicide gene showed a suppression of tumor growth in Huh7 tumor-bearing mice.…”

Section: Recent Designsmentioning

confidence: 99%

Polymeric Carriers for Nucleic Acid Delivery: Current Designs and Future Directions

Lun¹,

Wagner

2019

Biomacromolecules

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Within the last two decades, a series of novel therapeutic nucleic acids entered research and clinical evaluation. Their differences both in biophysical properties as well as in mode and site of biological action provide polymer-based carriers with new delivery challenges. Recent tailor-made designs of polymeric carriers are reviewed that were optimized for nucleic acid cargos such as plasmid DNA, siRNA, and micro RNA, mRNA, or genome-modifying nucleic acids. The specific requirements for the various therapeutic cargos are discussed. Future directions include dynamic bioresponsive polymers as components of nanomachines, multifunctional sequence-defined carriers for evolution-based selective optimization, and organic−inorganic multicomponent nanoassemblies.

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In vitro and in vivo gene delivery using chitosan/hyaluronic acid nanoparticles: Influences of molecular mass of hyaluronic acid and lyophilization on transfection efficiency

Abstract: The coating of pDNA/chitosan complexes with high-molecular-weight HA augmented the stability and cellular transfection ability of the complexes after lyophilization-rehydration.

Cited by 24 publications

References 36 publications

Identification and imaging of miR-155 in the early screening of lung cancer by targeted delivery of octreotide-conjugated chitosan-molecular beacon nanoparticles

Identification and imaging of miR-155 in the early screening of lung cancer by targeted delivery of octreotide-conjugated chitosan-molecular beacon nanoparticles

Hyaluronan as a Prominent Biomolecule with Numerous Applications in Medicine

Polymeric Carriers for Nucleic Acid Delivery: Current Designs and Future Directions

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