In vitro and in vivo biocompatibility testing of Ti‐6Al‐7Nb alloy with and without plasma‐sprayed hydroxyapatite coating

Abstract

The Ti-6Al-7Nb alloy has been recently developed for biomedical use, particularly for orthopedics and dental applications. Osteosynthesis has been used to analyze biocompatibility and osseoconduction properties. The interaction of the implant with its biological environment, the formation of the implant material/tissue interface, and the long-term success or failure of integration in the human body is strongly connected with the surface properties of the implant device. This study was undertaken to evaluate the processes involved in biological responses of the Ti-6Al-7Nb alloy with and without hydroxyapatite coatings with both in vitro and in vivo tests. The results were analyzed by scanning electron microscopy (SEM) and energy-dispersive x-ray (EDX) microanalysis. The morphology of the in vitro and in vivo testing results with hydroxyapatite coating was similar to those obtained on the uncoated samples. A mineralized extracellular matrix was formed on all materials. Observation of the interface between the cell layer and substrata showed the presence of calcium and phosphorous-rich globular deposits associated with collagen fibers on all materials in vitro and in vivo. A higher density of these globular deposits was observed in all samples.

Comparative assessment of structural and biological properties of biomimetically coated hydroxyapatite on alumina (α‐Al2O3) and titanium (Ti‐6Al‐4V) alloy substrates

“…[35][36][37][38][39] Most of the studies have been done on HAp coatings made on titanium metal alloys or collagen where the HAp deposition was done using different methodologies such as plasma coating, spin coating, and making HAp-titanium composites. 6 In vitro bioactivity of biomimetically coated HAp on titanium or ceramic substrates has been reported less-frequently; however, recently composites of HAp and proteins have been used as scaffolds to assess the in vitro behavior of cells.…”

Behaviour of Ti6Al4V implant alloy in vitro after plastic deformation by bending

“…It seems that the reconstruction of bi-layer surface structure on that sample was the quickest and contained oxides with HAp deposits, as it was reported earlier. [20] After 6 days of exposition to biological environment, the surface layer on the sample bent at the 20…”

“…The impedance of surface layer was estimated on the basis of Bode (−θ = f (log F) diagrams. [20,21] The electrochemical data were gathered and analysed using the 9831 FRA Frequency Response Analyzer and IMP 99 Software (ATLAS, Poland). Platinum plate as the counter-electrode and the saturated calomel electrode (SCE) as the reference electrode were used.…”

Development of a Compact Maglev Centrifugal Blood Pump Enclosed in a Titanium Housing

“…Ti-6Al-7Nb was chosen as a housing material due to its good biocompatibility properties, different resistivity compared with pure titanium, and the lower cytotoxicity characteristics of niobium compared with vanadium, which is present in Ti-6Al-4V and widely used as biomedical alloy (4), (10) . The resistivities of pure titanium and of Ti-6Al-7Nb, measured using a resistivity meter (Loresta GP -MCP T600, Dia Instruments, Japan), were 4.55×10 -7 Ω.m and 1.69×10 -6 Ω.m, respectively.…”

“…Titanium and some alloys of titanium are well known biomaterials which are present not only in heart valves and vascular stents (4), (5) , but also in maglev centrifugal blood pumps, such as HeartMate III (Thoratec Corp, U.S.A.) (6), (7) , and DuraHeart (TerumoHeart Inc., U.S.A.) (7), (8) . Although both these blood pumps have a titanium housing, there is no published reference on the influence of this housing on the sensitivity of the position sensors for the MB, on the stability of the MBs, nor on the energy losses of the drive motor.…”