In vitroandin vivoantitumor activity of the halogenated boroxine dipotassium-trioxohydroxytetrafluorotriborate (K2[B3O3F4OH])

Ivanković, Siniša; Stojković, Ranko; Galić, Zoran; Galić, Borivoj; Ostojić, Jelena; Marasović, Maja; Miloš, Mladen

doi:10.3109/14756366.2014.926344

Cited by 23 publications

(37 citation statements)

References 14 publications

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“…Preliminary investigations imply that acute toxicity of this substance is approximately 60 mg/kg suggesting reproducible biological effects on genetic material at low treatment concentrations (B. Galic, data not published). Previous findings of antitumor activity of the halogenated boroxine in vitro and in vivo on 4T1 mammary carcinoma, B16F10 melanoma and squamos cell carcinoma SCVII revealed inhibitory effects on cell proliferation in concentrations of 1 mM while concentrations of less than 0.1 mM do not significantly affect cell growth 6 . In vivo, K 2 (B 3 O 3 F 4 OH) slows the growth of three tested tumors compared to control regardless of the route of administration (intraperitoneally, intratumor, per oral or as an ointment).…”

Section: K 2 (B 3 O 3 F 4 Oh)mentioning

confidence: 85%

Effects of dipotassium-trioxohydroxytetrafluorotriborate, K₂[B₃O₃F₄OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line

Pojskić

Haverić

Lojo-Kadric

et al. 2015

Journal of Enzyme Inhibition and Medicinal Chemistry

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Recently it was found that dipotassium-trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), is a potent and highly specific inhibitor of precancerous cell processes. We conducted gene expression profiling of human melanoma cells before and after treatment with two concentrations (0.1 and 1 mM) of this boron inorganic derivative in order to assess its effects on deregulation of genes associated with tumor pathways. Parallel trypan blue exclusion assay was performed to assess the cytotoxicity effects of this chemical. Treatment with K2(B3O3F4OH) induced a significant decrease of cell viability in melanoma cellline at both tested concentrations. Furthermore, these treatments caused deregulation of more than 30 genes known as common anti-tumor drug targets. IGF-1 and hTERT were found to be significantly downregulated and this result may imply potential use of K2(B3O3F4OH) as an inhibitor or human telomerase and insulin-like growth factor 1, both of which are associated with various tumor pathways.

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Section: K 2 (B 3 O 3 F 4 Oh)mentioning

confidence: 85%

Effects of dipotassium-trioxohydroxytetrafluorotriborate, K₂[B₃O₃F₄OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line

Pojskić

Haverić

Lojo-Kadric

et al. 2015

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Effects of K 2 (B 3 O 3 F 4 OH) on the growth inhibition of tumor cells are significant in the growing medium containing low levels of Ca2+ ions (0.42 mM) (Ivankovic et al, 2016). In vivo, regardless of the administration mode used (intraperitoneally, intratumor, per os or topical) K 2 (B 3 O 3 F 4 OH) slowed the growth of all three tested tumor types compared to the controls (Ivankovic et al, 2015). Significant decrease of cell viability for concentrations of 0.025 and 0.25 mg/ml of K 2 (B 3 O 3 F 4 OH) was also shown in human Caucasian melanoma cell line (GR-M).…”

Section: Introductionmentioning

confidence: 97%

In Vitro Study of the Anti-Proliferative Effects of Dipotassium-Trioxohydroxytetrafluorotriborate on the H520 Non-Small Cell Lung Cancer Cell Line

Liu

Saračević

Burnett

et al. 2017

GenApp

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Non-small cell lung cancer has been shown to be resistant to many forms of chemotherapy and is amongst the deadliest cancers worldwide. The antiproliferative effects of halogenated boroxine -K 2 (B 3 O 3 F 4 OH), have been confirmed in multiple cancer cell lines including melanoma and breast cancer. The potential for this chemical treatment on non-small cell lung cancer cells was studied and the lower threshold concentration with the clear biological effect of halogenated boroxine, was determined. Appling MTT assays and the relative gene expression analysis of two genes of interest, RRBP1 and PER1, novel knowledge on the biological potential of halogenated boroxine (HB) was gained, but did not lead to biological explanations of the mechanisms of halogenated boroxine activity. The results of MTT assay showed a significant HB effect on non-small cell lung cancer in concentration of 0.5 mg/mL, while relative expression levels of RRBP1 and PER1 did not significantly differ regardless the concentration applied.

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“…Islamovic et al (2014) and Vullo et al (2015) investigated the kinetic parameters and mechanism of inhibition of enzymes, catalase and human carbonic anhydrases, in the presence of K2[B3O3F4OH]. Ivankovic et al (2015) showed the potent antitumor activity of K2[B3O3F4OH] that is comparable to the well-known anticancer drug, 5-fluorouracil. The antitumor activity was tested in vitro on a number of tumor cell lines (adenocarcinoma 4T1, melanoma B16F10, and squamous cell carcinoma SCCVII) and in vivo on solid tumors of the same type in syngeneic mice.…”

Section: Introductionmentioning

confidence: 99%

Determination of Antioxidant Capacity of Selected Boroxines

Marasović

Roščić

Galić

et al. 2017

CBUP

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. In our research, we include two other simpler representatives of the boroxine family compounds: trimethoxyboroxine and trimethylboroxine, which are commercially available. The study objective is to explore the possibility of similar behavior within the same class of boron compounds, that is, to examine the activity of K2[B3O3F4OH] compared to simpler representatives of the same family of compounds. On the one hand, K2[B3O3F4OH], theoretically has the ability to exchange electrons in the extinction of reactive radicals, since two boron atoms are sp3-hybridized and use electrons from the inner shell. On the other hand, trimethoxyboroxine, and trimethylboroxine, in theory, should not exchange electrons. However, recent studies indicate the potential for the boron atom to act like carbon and participate in the exchange of protons. The study used the standard laboratory method of 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay. The selected boroxines were treated with a DPPH radical at a temperature of 35° C in various concentrations, and with a reaction time of one hour. Results of the DPPH test show an extremely weak antioxidant capacity exists for all investigated boroxines. When K2[B3O3F4OH] was tested at high concentrations, instead of decreased color in the DPPH radicals, there was an increase in absorbance readings, which could mean that this compound acts as a pro-oxidant at higher concentrations. Future research is recommended to examine the length of reaction times needed, and whether a change in the reaction conditions would boost the antioxidant capacity of K2 [B3O3F4OH]. Finally, future research could test the hypothesis that K2[B3O3F4OH], in the absence of the expected antioxidant activity, acts as a pro-oxidant.UDC Classification: 615.1; DOI: http://dx

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In vitroandin vivoantitumor activity of the halogenated boroxine dipotassium-trioxohydroxytetrafluorotriborate (K₂[B₃O₃F₄OH])

Cited by 23 publications

References 14 publications

Effects of dipotassium-trioxohydroxytetrafluorotriborate, K₂[B₃O₃F₄OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line

Effects of dipotassium-trioxohydroxytetrafluorotriborate, K₂[B₃O₃F₄OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line

In Vitro Study of the Anti-Proliferative Effects of Dipotassium-Trioxohydroxytetrafluorotriborate on the H520 Non-Small Cell Lung Cancer Cell Line

Determination of Antioxidant Capacity of Selected Boroxines

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In vitroandin vivoantitumor activity of the halogenated boroxine dipotassium-trioxohydroxytetrafluorotriborate (K2[B3O3F4OH])

Cited by 23 publications

References 14 publications

Effects of dipotassium-trioxohydroxytetrafluorotriborate, K2[B3O3F4OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line

Effects of dipotassium-trioxohydroxytetrafluorotriborate, K2[B3O3F4OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line

In Vitro Study of the Anti-Proliferative Effects of Dipotassium-Trioxohydroxytetrafluorotriborate on the H520 Non-Small Cell Lung Cancer Cell Line

Determination of Antioxidant Capacity of Selected Boroxines

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In vitroandin vivoantitumor activity of the halogenated boroxine dipotassium-trioxohydroxytetrafluorotriborate (K₂[B₃O₃F₄OH])

Effects of dipotassium-trioxohydroxytetrafluorotriborate, K₂[B₃O₃F₄OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line

Effects of dipotassium-trioxohydroxytetrafluorotriborate, K₂[B₃O₃F₄OH], on cell viability and gene expression of common human cancer drug targets in a melanoma cell line