In vitroandin vivocomparison of the biological activities of two traditionally and widely usedArumspecies from Jordan:Arum dioscoridisSibth &amp; Sm. andArum palaestinumBoiss.

Afifi, Fatma U.; Kasabri, Violet; Liţescu, Simona Carmen; Abaza, Ismail M

doi:10.1080/14786419.2015.1072713

Cited by 16 publications

(21 citation statements)

References 23 publications

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“…In Jordan, it is represented by three species: A. palaestinum , A. dioscoridis , and A. hygrophilum . [ 22 ] In our previous investigations with the former two species, several flavonoids were isolated, and subsequently flavonoids, coumarins, and plant acids were identified using LC-MS.[ 8 9 ] The isolated isoorientin showed myolytic activity on smooth muscle containing preparations from the rat and guinea pig. [ 23 ] Also, the antiproliferative activities of both species were evaluated using different cancer cell lines.…”

Section: Resultsmentioning

confidence: 99%

“…[ 23 ] Also, the antiproliferative activities of both species were evaluated using different cancer cell lines. [ 9 24 ]…”

Section: Resultsmentioning

confidence: 99%

“…[ 8 ] In a recent comparative study in in vitro and in vivo experiments, pancreatic lipase (PL) and dual α-amylase/α-glucosidase inhibitory potentials were demonstrated for the extracts of A. dioscorides and A. palaestinum as well as for some of their isolated compounds. [ 9 ] Earlier, Karahan et al . [ 10 ] reported free radical scavenging and ferric-reducing activities of ethanol, methanol, acetone, and water extracts of A. dioscoridis leaves.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical and biological evaluations of Arum hygrophilum boiss. (Araceae)

et al. 2017

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Background:Arum hygrophilum is a traditional medicinal plant indigenous to Jordan. The present study explores its phytochemistry, antioxidative, antidiabesity, and antiproliferative potentialities.Materials and Methods:Column chromatography and HPLC-MS analysis were used for its phytochemical evaluation. Using leaf crude water and ethanol extracts, the antioxidative capacities, their modulation of pancreatic β-cell proliferation, and insulin secretion as well as glucose diffusion and enzymatic bioassays were evaluated.Results:Three flavonoids (luteolin, isoorientin, and vitexin) and β-sitosterol have been isolated and their structures determined. HPLC-MS analysis of the ethanol extract further revealed the presence of caffeic, ferulic, gallic, and rosmarinic acids and quercetine-3-O-rhamnoside. The ethanol extract exhibited DPPH and ABTS radical scavenging and antioxidative capacities. A. hygrophilum (1), vitexin (2), and rosmarinic acid (3) inhibited pancreatic lipase (PL) dose dependently with PL-IC50 (µg/mL) values in an ascending order: (3); 51.28 ± 7.55 < (2); 260.9 ± 21.1 < (1); 1720 ± 10. Comparable to GLP-1-enhanced β-cell proliferation in 2-day treatment wells, a dose-dependent augmentation of BrdU incorporation was obtained with the A. hygrophilum aqueous extract (AE) (0.5 and 1 mg/mL, with respective 1.33- and 1.41-folds, P < 0.001). A. hygrophilum AE was identified as an inhibitor of α-amylase/α-glucosidase with IC50 value of 30.5 ± 2.1 mg/mL but lacked antiproliferative effects in colorectal cancer cell lines (HT29, HCT116, and SW620) and insulinotropic effects in β-cell line MIN6.Conclusion:A. hygrophilum extracts inhibited gastrointestinal enzymes involved in carbohydrate and lipid digestion and absorption.SUMMARY Phytochemical evaluation of Arum hygrophilum recovered flavonoids (luteolin, isoorientin and vitexin) and β-sitosterolHPLC-MS analysis of its antioxidative ethanol extract further revealed the presence of caffeic-, ferulic-, gallic- and rosmarinic acids and quercetine-3-O-rhamnosideA. hygrophilum inhibited α-amylase/α-glucosidase and pancreatic lipase dose-dependentlyA. hygrophilum augmented β-cell proliferation dose dependently, but it lacked antiproliferative effects in colorectal cancer cell lines (HT29, HCT116, and SW620) and insulinotropic effects in β-cell line MIN6 Abbreviations used: ABTS: 2,2’-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid, AE: Aqueous Extract, ANOVA: Analysis Of Variance, AUC: Area Under Curve, BrdU: 5-Bromo-2’-Deoxyuridine, DPPH: 2,2-Diphenyl -1-Pycriylhydrazyl, ELISA: Enzyme Linked Immunosorbent Assay, GLP1: Glucagon Like Peptide 1, GSIS: Glucose Stimulated Insulin Secretion, HPLC-MS: High Performance Liquid Chromatography –Mass Spectrometry, IC50: 50% Inhibitory Concentration, KRH: Krebs/Ringer/Hepes, MTT: 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide, OGTT: Oral Glucose Tolerance Test, ORAC: Oxygen Radical Antioxidant Capacity, OSTT: Oral Starch Tolerance Test, PL: Pancreatic Lipase, SEM: Standard Error Of The Mean, SRB: Sulforhodamine B, TE...

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Section: Resultsmentioning

confidence: 99%

“…[ 23 ] Also, the antiproliferative activities of both species were evaluated using different cancer cell lines. [ 9 24 ]…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phytochemical and biological evaluations of Arum hygrophilum boiss. (Araceae)

et al. 2017

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“…In vitro enzymatic starch digestion assay: The extent of the polysaccharide breakdown into glucose was evaluated in a concentration range of either plant AEs or acarbose at 1000 μg/ mL concentration, in triplicate, as described [15].…”

Section: Spectrophotometric Quantification Of Pancreatic Lipase (Pl) mentioning

confidence: 99%

“…In vitro antiproliferative assay: Cytotoxicity measurements in colorectal cell lines HT29, HCT116 and SW620 were determined using the Sulforhodamine B (SRB) colorimetric assay [15]. Doxorubicin IC 50 values, were calculated within the treatment concentration range 0.1-50 μg/mL.…”

Section: Spectrophotometric Quantification Of Pancreatic Lipase (Pl) mentioning

confidence: 99%

Composition and Biological Activity of Volatile Oil from Salvia judaica and S. multicaulis from Jordan

Kasabri

et al. 2016

Natural Product Communications

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The aim of this work was to determine the composition of the hydro-distilled essential oil of Salvia judaica Boiss. and S. multicaulis Vahl. (Lamiaceae) from Jordan by GC and GC-MS and to report the actual composition of their fresh leaves and flowers using SPME (Solid Phase Micro-Extraction).Their dual alphaamylase/alpha glucosidase and pancreatic lipase inhibitory activities as well as their anti-proliferative potential were screened. The aroma profile of the leaves, flowers, and flowers at pre-flowering stages of S. judaica, obtained through SPME was composed of sesquiterpene hydrocarbons (87.7 %, 71.8 %, and 86.2 %, respectively) while the hydro-distilled oil of the dry leaves was rich in oxygenated sesquiterpenes (50.8%). Fresh leaves of S. multicaulis were rich in oxygenated monoterpenes (58.1%), while monoterpene hydrocarbons dominated the blooming flowers (57.2%) and the flowers at the pre-flowering stage (64.7%). The hydro-distilled oil of the dry leaves was rich in oxygenated monoterpenes (77.6%). With doxorubicin as a positive control, no anti-proliferative activity was observed against colorectal cancer cell lines HT29, HCT116, and SW620 using SRB assay for either Salvia spp. In vitro enzymatic starch digestion was evaluated with Acarbose (IC 50 : 0.2± 0.0 µg /mL) as the reference drug. The respective IC 50 (mg/mL) values of S. judaica and S. multicaulis aqueous extracts were 4.9 ± 0.4 and 10.3 ± 0.9. Modulation of pancreatic lipase activity (PL) was determined by colorimetry and compared with Orlistat (IC 50 : 0.11 ± 0.0 μg/mL). PL-IC 50 values (μg/mL) obtained for S. judaica and S. multicaulis were 108.5±6.4 and 31.8 ± 0.8, respectively.

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