<i>In vitro</i> anthelmintic and cytotoxic activities of extracts of <i>Persea willdenovii</i> Kosterm (Lauraceae)

Rosa, S.S. Santa; Santos, Francianne Oliveira; Lima, Hernando Nascimento; Reis, Isabella Mary Alves; Cassiano, Dayse Santos Almeida; Vieira, Ivo José Curcino; Braz-Filho, Raimundo; Uzêda, Rosângela Soares; Botura, Mariana Borges; Branco, Alexsandro; Batatinha, Maria José Moreira

doi:10.1017/s0022149x17000979

“…This mean hatching inhibition rate seen in the wells containing the extracts confirms the fact that P. americana seed extracts possess egg hatching inhibitory power. Wabo et al [ 27 ] obtained similar results with Chenopodium ambrosiodes on H. bakeri eggs and Rosa et al [ 28 ] when evaluating Persea willdenovii extracts on gastrointestinal nematodes. The egg hatching rates of H. polygyrus had IC 50 of 0.49 mg/mL and 0.22 mg/mL for the ethanol and aqueous extracts, respectively.…”

Section: Discussionmentioning

confidence: 64%

Nematocidal Activity of Ethanol and Aqueous Extracts of Persea americana Seeds against Heligmosomoides polygyrus using the Worm Microtracker Method

Cédric,

Nadia,

Nfufu

et al. 2023

Journal of Parasitology Research

1

0

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Background. Infections with gastrointestinal helminths constitute a serious obstacle to the good health of the local population in most African Countries. The aim of this study was to evaluate the anthelminthic activity of Persea americana ethanol and aqueous extracts against Heligmosomoides polygyrus using the worm microtracker. Method. Aqueous and ethanolic extracts of P. americana were prepared. Different concentrations of the extracts were tested against the egg and larvae stages of H. polygyrus using an automated high-throughput method. Briefly, embryonated eggs and larvae of this parasite were obtained after the incubation of fresh eggs at 25°C for 24, 48, and 96 hours for embryonated eggs, L1 and L2 larvae, respectively. One hundred microliters of the plant extracts at various concentrations were put in contact in a 96-well microplate with a suspension of 100 embryonated eggs in a total volume of 200 μL and incubated in a worm microtracker where the motility of the worms was recorded every 30 minutes for the ovicidal activity. The final tested extract concentration was 5, 2.5, 1.25, 0.625, and 0.3125 mg/mL, whereas ringer solution (0.95%) and 1.5% Dimethyl sulfoxide (DMSO) were used as negative controls and levamisole as positive control. The same method was used for larvicidal activities. The anthelmintic activity was determined using the average movement of the worms in the tested product compared with the negative control (1.5% DMSO and ringer solution). Results. The egg hatching rates of H. polygyrus had IC50 of 0.49 mg/mL (95% confidence interval: 71.70–92.03) and 0.22 mg/mL (95% confidence interval: 74.28–86.18) for the ethanol and aqueous extract, respectively. These IC50 indicate that the aqueous extract is more active for the inhibition of hatching at a 95% confidence interval. The aqueous and ethanol extracts presented mean inhibitory hatching rates of 78.33 ± 1.67 % and 75.67 ± 1.15 % at 5 mg/mL, respectively, with no significant differences. The highest percentage of inhibition of L1 larva was observed at 5 mg/mL with 89 ± 2.3 % and 85 ± 2.7 % for the ethanol and aqueous extracts, respectively. The lowest percentage of inhibition was observed at 0.3125 mg/mL, with 54.67 ± 3.38 % and 49 ± 2.64 % for the ethanol and aqueous extract, respectively. No significant differences were observed between the two extracts at 5 mg/mL with an inhibitory percentage of 90.67 ± 3.05 % (ethanol) and 89.33 ± 2.08 % (aqueous). Conclusion. Extracts of P. americana seeds possess nematocidal activity, however, further in silico and in vivo investigations are necessary to confirm their anthelminthic activity.

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“…This plant is a source of bioactive compounds especially antioxidants and its seed extracts have been proven to possess various therapeutic activities (Dabas et al, 2013;Melgar et al, 2018;Widiyastuti et al, 2018;Bhuyan et al, 2019;Pacheco-Coello & Seijas-Perdomo, 2020). Moreover, the antihelmithic activity for avocado was reported previously via Rosa et al (2018) against goat gastrointestinal nematodes and Soldera-Silva et al ( 2018) against third-stage larvae of Haemonchus contortus. For this, avocado has gained worldwide recognition and its consumption has considerably increased in the last years.…”

Section: Introductionmentioning

confidence: 94%

Biological activities of Persea americana: in vitro and in vivo studies

Al-Otaibi

¹

,

Hawsah

²

,

Alojayri

³

et al. 2023

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Natural resources emerge as an essential and alternative tool for controlling parasitic agents. The present study was carried out to evaluate in vitro and in vivo effect of Persea americana fruit extract (PAFE) on helminthiasis and coccidiosis. In vitro, anthelmintic activity was performed using PAFE against Allolobophora caliginose. PAFE elicited significant anthelmintic activity comparable to mebendazole with the lesser time taken to paralysis and death for 200 mg/mL. In vitro anticoccidial activity of PAFE, amprolium, phenol, Dettol TM , and formalin were studied after incubation with unsporulated oocysts. PAFE was able to significantly inhibit oocyst sporulation in a dose-dependent manner. A total 35 male mice were divided into seven groups. At day 0, all groups except first and second groups were infected with 1×10 3 E. papillata. Group 1 served as control. Group 2 served as uninfected-treated one. Group 3 was considered an infected-untreated group. After 60 min of infection, groups 4, 5, and 6 were treated with PAFE. Group 7 was treated with amprolium. However, not at par with amprolium, PAFE showed a good anticoccidial activity adjudged based on a significant change in body weight gain, feed intake, oocysts output, and content of carbohydrates and protein. Therefore, PAFE exhibits powerful anthelmintic and anticoccidial effects.

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“…[76] The species P. indica is a rich source of diterpenes in the genus Persea, [13,18,20] particularly those related to the ryanodan skeleton, such as ryanodol (5), which was first isolated as a natural product in this species. [20] Additionally, other diterpenic derivatives of ryanodane skeleton have also identified, such as cinnzeilanol (6), ryanodol-14-monoacetate (7), cinnzeilanine (8), epi-cinnzeilanol (9), cinnzeilanone (10), indicol (11), vignaticol (12), and perseanol (13). [19] Koshimizu et al (2016) reviewed, by chemical synthesis, the structure of "natural ryanodol" (5) as 3-epi-ryanodol (also named 14-epi-ryanodol).…”

Section: P Americanamentioning

confidence: 99%

“…[3] Inserted in the Lauraceae family, the genus Persea has approximately 200 species and is distributed mainly in tropical and subtropical Americas and Asia. [4,5] Antispasmodic, [6] antioxidant, [7] gastroprotective, [8] cytotoxic, [9,10] anthelmintic, [10] and inseticide [11][12][13][14][15] properties are among the biological activities described for species of the genus Persea. Considering the significant contribution of this genus to the search for new substances with biological application, we present in this review information the substances isolated from Persea species, as well as their action in various models of biological activity.…”

Section: Introductionmentioning

confidence: 99%

Chemistry and Bioactivity of the Genus Persea ‐ A Review

Totini,

Umehara,

Reis

et al. 2023

Chemistry & Biodiversity

3

0

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This review provides the first comprehensive appraisal of bioactive compounds and their biological activities in Persea species from 1950 to 2023. Relevant articles from reputable databases, including PubMed, Web of Science, Science Direct and Google Scholar were collected, leading to the isolation of about 141 metabolite compounds, mainly flavonoids, terpenoids, fatty alcohols, lignoids, and γ‐lactone derivatives. These compounds exhibit diverse biological activities, including insecticidal, antifeedant, nematicidal, antibacterial, antifungal, antiviral, cytotoxic, anti‐inflammatory, and antioxidant properties. The review emphasizes the significant chemical and pharmacological potential of different Persea species, encouraging further research in various fields and medicine. Valuable insights into potential applications of Persea plants are provided.

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In vitro anthelmintic and cytotoxic activities of extracts of Persea willdenovii Kosterm (Lauraceae)

Cited by 10 publications

References 31 publications

Nematocidal Activity of Ethanol and Aqueous Extracts of Persea americana Seeds against Heligmosomoides polygyrus using the Worm Microtracker Method

Nematocidal Activity of Ethanol and Aqueous Extracts of Persea americana Seeds against Heligmosomoides polygyrus using the Worm Microtracker Method

Biological activities of Persea americana: in vitro and in vivo studies

Chemistry and Bioactivity of the Genus Persea ‐ A Review

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