<i>In vitro</i>Anti-Influenza Viral Activities of Constituents from<i>Caesalpinia sappan</i>

Liu, Ailin; Shu, Shi-Hui; Qin, Hai‐Lin; Lee, Simon Ming‐Yuen; Wang, Ying; Du, Guanhua

doi:10.1055/s-0028-1112208

Cited by 82 publications

(61 citation statements)

References 3 publications

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“…Brazilin (in SFN) has been reported to have many biological activities, including immune regulation, 19,20 blood vessel dilatation, 3,11,14 anticoagulation, 8,17 hypoglycemic effect, 10,13,15,16,26,27 oxidative damage inhibition, 6,28 antibiosis, 9 and anti-inflammatory effects. 4,5,7 In addition, it was demonstrated that brazilin powerfully broke deoxynucleic acid chains in anticancer screening.…”

Section: Discussionmentioning

confidence: 99%

“…Brazilin was identified as one of the most active constituents in sappan wood. 7 In recent studies, the anti-inflammatory effects of brazilin in macrophages via novel mechanisms involving heme oxygenase-1 (HO-1) 8 and inducible nitric oxide synthase (iNOS) gene expression 9 were reported. The suppressive effect on iNOS gene expression by brazilin might provide a possible mechanism for its chemopreventive activity in cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Su Fu’ning Lotion’s Inhibitory Effects on Bladder Cancer Cells In Vitro and In Vivo by Intravesical Instillation

et al. 2015

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Background. Bladder cancer is a common malignant tumor with a very high recurrence rate after surgery. Intravesical instillation can help clear up the residual tumor cells after surgery and thereby reduce the recurrence rate. Objective. To establish a bladder tumor transplantation animal model and to evaluate the inhibitory effects of a novel perfusate, Su Fu'ning Lotion (SFN), on bladder tumor. Methods. SFN was compared with several commonly used chemotherapy drugs, including mitomycin (MMC) and pirarubicin (THP) for anticancer effects on the bladder cancer cell lines T24, BTT, and BIU-87 and SFN half inhibitory concentrations (IC 50 ) were determined after 48 hours of treatment. In addition, bladder cancer orthotopic transplantation tumor models were established in BALB/C nude mice and T739 mice, and SFN anticancer effects were assessed in vivo, with normal saline and MMC as negative and positive controls, respectively. Results. SFN, MMC, and THP were all lethal to bladder cancer cells, in vitro, with SFN and THP significantly superior to MMC. IC 50 values for SFN were 13.22, 11.22, and 12.5 µg/mL on T24, BTT, and BIU-87 cells, respectively. In vivo, SFN significantly reduced the mouse bladder wet weight and prolonged the animal survival compared with controls (P < .05), suggesting that SFN significantly inhibited T24/BTT cell growth in mice. Conclusion. SFN inhibited the bladder cancer cell proliferation in vitro and in vivo and significantly prolonged the survival of mice with bladder cancer xenografts, indicating that SFN could be used as a perfusate after surgery for removal of residual bladder cancers cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Su Fu’ning Lotion’s Inhibitory Effects on Bladder Cancer Cells In Vitro and In Vivo by Intravesical Instillation

et al. 2015

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“…(Leguminosae) is distributed in Southeast Asia. It is used as an herbal medicine for the treatment of inflammation and to improve blood circulation (Nagai et al, 1984), influenza, allergic, and neuroprotective activities (Liu et al, 2009;Shen et al, 2007;Yodsaoue et al, 2009). This plant was used in Vietnamese traditional medicine for the treatment of menstrual and post-partum hematometra, trauma blood static, dizziness, post-partum blood losses, bloody dysentery, enteralgia, intestinal hemorrhage, infectious diarrhea, and for washing wounds (Bich, 1999).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic constituents from the seeds of VietnameseCaesalpinia sappan

Tran

Nguyen

et al. 2015

Pharmaceutical Biology

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Context: Caesalpinia sappan Linn. (Leguminosae) has been used in folk medicines for the treatment of many diseases. The heartwood of this plant contains various phenolic components with interesting biological applications; however, the chemical and biological potentials of the seed of this plant have not been fully explored.Objective: This study identified the cytotoxic activity of compounds from the seeds of C. sappan. Materials and methods: The methanol extract of the seed of C. sappan was suspended in H 2 O and then partitioned with CH 2 Cl 2 , EtOAc, and n-BuOH, successively. Diterpenoid compounds were isolated from the CH 2 Cl 2 -soluble fraction by silica gel column chromatography methods using organic solvents. The compound structures were determined by detailed analysis of NMR and MS spectral data. Cytotoxic activity was measured using a modified MTT assay against HL-60, HeLa, MCF-7, and LLC cancer cells. The activation of caspase-3 enzyme and western blotting assay were performed to confirm inhibitory mechanism of active compound. Results: Five cassane-type diterpenoids were isolated and identified as phanginin I (1), phaginin A (2), phanginin D (3), phanginin H (4), and phanginin J (5). Compounds 1-4 showed effective inhibition against HL-60 cells with the IC 50 values of 16.4 ± 1.5, 19.2 ± 2.0, 11.7 ± 1.6, and 22.5 ± 5.1 mM. Compounds 1-3 exhibited cytotoxic activity against HeLa cells with the IC 50 values of 28.1 ± 3.6, 37.2 ± 3.4, and 22.7 ± 2.8 mM. Treatment of HL-60 cell lines with various concentrations of 3 (0-30 mM) resulted in the growth inhibition and induction of apoptosis.Conclusion: These findings demonstrate that compound 3 (phanginin D) is one of the main active components of the seed of C. sappan activating caspases-3 which contribute to apoptotic cell death.

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“…Recently, the anti-influenza viral activity, 3) the anti-allergic activity, 4) and the neuroprotective activity 5) of Sappan Lignum or brazilin, the major constituent of Sappan Lignum, were reported. Although other compounds, including sappanchalcone and protosappanins A-E, are present in Sappan Lignum, there are few reports of their pharmacological activities.…”

mentioning

confidence: 99%

Vasorelaxant Activity of Sappan Lignum Constituents and Extracts on Rat Aorta and Mesenteric Artery

Sasaki

Suzuki

Matsumoto

et al. 2010

Biological & Pharmaceutical Bulletin

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We investigated the vasorelaxant activity of the methanolic extracts of Sappan Lignum (CSE) and its constituents, brazilin, sappanchalcone, and protosappanins A-E, on rat aorta and mesenteric artery. By comparing the vasorelaxant activity of CSE and brazilin on both blood vessels, we found that CSE contained active constituents other than brazilin. When added to brazilin, sappanchalcone and protosappanin D showed vasorelaxant activity on both blood vessels precontracted with phenylephrine. We clarified that the vasorelaxant activity of brazilin was endothelium-independent, while that of sappanchalcone was endothelium-dependent, on both blood vessels. On the other hand, the vasorelaxant activity of protosappanin D was independent of the endothelium of the aorta and dependent on the endothelium of the mesenteric artery. Experiments on sappanchalcone and protosappanin D using N G -nitro-L-arginine and indomethacin revealed the involvement of nitric oxide and prostaglandin as endothelium-derived relaxation factors (EDRFs). The anti-oketsu effect of Sappan Lignum might be attributable to the interaction of those compounds. We could partly evaluate the anti-oketsu activity of Sappan Lignum using both the aorta and the mesenteric artery. Through this study, we showed the importance of comparing the effects on the aorta and the mesenteric artery as we found that natural compounds showed different mechanisms of action on the two blood vessels.

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In vitroAnti-Influenza Viral Activities of Constituents fromCaesalpinia sappan

Cited by 82 publications

References 3 publications

Evaluation of Su Fu’ning Lotion’s Inhibitory Effects on Bladder Cancer Cells In Vitro and In Vivo by Intravesical Instillation

Evaluation of Su Fu’ning Lotion’s Inhibitory Effects on Bladder Cancer Cells In Vitro and In Vivo by Intravesical Instillation

Cytotoxic constituents from the seeds of VietnameseCaesalpinia sappan

Vasorelaxant Activity of Sappan Lignum Constituents and Extracts on Rat Aorta and Mesenteric Artery

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