In VitroAntiallergic Effects of Aqueous Fermented Preparations fromCitrusandCydoniafruits

Abstract

This study aimed to investigate the immunomodulatory and antiallergic properties of preparations from lemon, Citrus medica L. (citrus), and quince, Cydonia oblonga Mill. (Cydonia), which are used in pharmaceutical products to treat patients suffering from allergic disorders. Preparations were analyzed with respect to their impact on the degranulation capacity from basophilic cells as well as mediator release from activated human mast cells in vitro, including IL-8 and TNF- α secretion. The results show that the degranulation of basophilic cells was diminished only in the presence of Citrus, and this effect was compared to the synthetic drug azelastine. Furthermore, Citrus and Cydonia both inhibited the production of IL-8 and TNF- α from human mast cells, and at low concentrations additive effects were observed. As a positive inhibition control, dexamethasone was used. LC-MS analyses showed that the major phenolic components in extracts from Citrus and Cydonia are eriocitrin and neochlorogenic acid, respectively. Nevertheless, these compounds do not show biological effects at concentration levels detected in their corresponding extracts. In conclusion, the present data provide a rational base for the use of the single pharmaceutical preparations from Citrus and Cydonia in a differentiated treatment of allergic disorders in part by the regulation of soluble allergic mediators from basophilic cells and mast cells.

Efficacy of a Nasal Spray fromCitrus limonandCydonia oblongafor the Treatment of Hay Fever Symptoms-A Randomized, Placebo Controlled Cross-Over Study

“…HPLC analysis of the study medication. Unneeded samples of the study medication were freeze dried and analyzed by PhytoLab GmbH (Vestenbergsgreuth, Germany) on content of eriocitrin and hesperidin as main active substances of C. limon (Caristi et al, 2003;Qiao et al, 2014) and chlorogenic acid derivates (Huber et al, 2012) as characteristic active components of the Cydonia oblongata component.…”

“…The effective in vitro concentrations (0.2-0.8 mg/mL) were in a range that could be reached by topical application as nasal spray. Furthermore, the lemon and quince components had in vitro differential and synergistic antiallergic effects (Baars et al, 2012;Huber et al, 2012). So far efficacy in humans has only been investigated in two (n = 12, n = 140) observational studies (Baars and De Bruin, 2005;Rother and Oexle, 2008) and a small (n = 23) randomized trial comparing the nasal and subcutaneous routes of application (Baars et al, 2011).…”

Effects of extracts and compounds from Tricholoma populinum Lange on degranulation and IL-2/IL-8 secretion of immune cells

“…Test modalities were described earlier [19]. A total of 10 5 HMC-1 cells were incubated in a 96-well plate for 30 min with different extract concentrations.…”

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

“…Aqueous preparations from whole quince fruits have been reported to be effective to treat allergic disorders [9]. Finally, a lipophilic quince extract was demonstrated to exhibit skin moisturizing properties [10]. Moreover, in the work by Umar et al [11] it is shown that Cydonia obonga (fruit and leaves) could regulate the blood lipid metabolism in using hyperlipidemic rat models while removing the oxygen free radicals and improving the antioxygenic potentials.…”