<i>In Vitro</i>Antibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates of<i>Stenotrophomonas maltophilia</i>including the Trimethoprim/Sulfamethoxazole Resistant Strain

Karunanidhi, Arunkumar; Thomas, R. Brent; Belkum, Alex van; Neela, Vasantha Kumari

doi:10.1155/2013/392058

Cited by 101 publications

(82 citation statements)

References 30 publications

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“…Consistent with our results, caffeic acid content level decreased in hairy roots (661.50 μg/g) compared to nontransformed roots (715.25 μg/g). Previously demonstrated that chlorogenic acid and chlorogenic acid containing plant materials have been shown to have antiviral, antifungal, and strong antibacterial activities (Karunanidhi et al, 2013). Salicylic acid and gentisic acid were predominant hydroxybenzoic acid groups in hairy roots (999.00 and 758.15 μg/g) and non-transformed roots (741.00 and 553.85 μg/g) followed by gallic acid, p-hydroxybenzoic acid, syringic acid, protocatechuic acid, β-resorcylic acid and vanillic acid.…”

Section: Comparison Of Phenolic Compounds In Transgenic Hairy Roots Amentioning

confidence: 99%

Evaluation of phenolic compounds, antioxidant and antimicrobial activities from transgenic hairy root cultures of gherkin ( Cucumis anguria L.)

Yoon

Chung

Thiruvengadam

2015

South African Journal of Botany

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a b s t r a c tThe production of secondary metabolites from hairy root cultures of gherkin (Cucumis anguria L.) along with their antioxidant and antimicrobial activities were studied. The explants from in vitro seedlings were inoculated with Agrobacterium rhizogenes strain KCTC 2703 for the induction of hairy roots. Transgenic clones of hairy roots were confirmed by polymerase chain reaction (PCR) and reverse transcription-PCR using rolC specific primers. Transgenic hairy roots developed in MS liquid medium supplemented with 3% sucrose exhibited the highest accumulation of biomass 98.55 g/l fresh mass (FM) and 10.15 g/l dry mass (DM) were observed at 21 days. Biomass accumulation of hairy root lines was 9.3-fold higher compared to in vitro grown roots. Moreover, transgenic hairy roots effectively produced several phenolic compounds, such as flavonols, hydroxycinnamic and hydroxybenzoic acids derivatives. Due to these metabolic changes, the antioxidant and antimicrobial activities were increased with hairy roots compared to non-transformed roots. The total phenolics, flavonoids content, antioxidant and antimicrobial activity were higher in hairy roots compared to non-transformed roots. Our study described the greater potentiality of C. anguria hairy root cultures for the production of important phenolic compounds and uses of their biological activities.

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Section: Comparison Of Phenolic Compounds In Transgenic Hairy Roots Amentioning

confidence: 99%

Evaluation of phenolic compounds, antioxidant and antimicrobial activities from transgenic hairy root cultures of gherkin ( Cucumis anguria L.)

Yoon

Chung

Thiruvengadam

2015

South African Journal of Botany

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“…Chlorogenic acid is a polyphenolic compound that forms an ester with caffeic acid with the 3-hydroxyl group of a quinic acid. It was demonstrated to have various health benefits including antiviral, antioxidant, antibacterial, antifungal, and other biological activities [13,14]. Furthermore, caffeic acid and chlorogenic acid were also found to be anticariogenic compounds [15,16].…”

Section: Discussionmentioning

confidence: 99%

Detection of caffeic and chlorogenic acids from methanolic extract of Annona squamosa bark by LC-ESI-MS/MS

Salman¹,

Ramasamy²,

Mahmood³

2018

J Intercult Ethnopharmacol

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Aim:The aim of the present study was to determine the metabolite profile of Annona squamosa bark using high-performance liquid chromatography-electrospray ionizationtandem mass spectrometry (LC-ESI-MS/MS). Methods: The plant material of A. squamosa bark was collected and processed during the month of February. The bark material was extracted by Soxhlet apparatus using methanol as a solvent. The metabolite profile of the plant extract was determined by using LC-ESI-MS/MS. Results: Caffeic and chlorogenic acids were detected from the methanolic extract of A. squamosa bark. To the best of our knowledge, this is the first study to report the presence of caffeic and chlorogenic acids in A. squamosa bark. Conclusion:The study suggested further investigations to be carried out to evaluate these compounds in vitro and in vivo to develop the pharmaceutical products. ARTICLE HISTORY

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“…Chlorogenic acid (3-O-caffeoylquinic acid), which is formed from caffeic and quinic acids is one of the major phenolic components seen in tobacco leaves and coffee seeds. Chlorogenic acid has multipharmacological properties such as antimicrobial 39 , anti-inflammatory effects 40,41 and cardioprotective effects 42,43 . It is a potent antioxidant, widespread in fruits and vegetables.…”

Section: Discussionmentioning

confidence: 99%

Anticancer and antiangiogenic effects of methanol extracts of Lonicera caprifolium L. on C6 rat glioma cells

et al. 2016

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SUMMARYObjective: Gliomas are brain tumors with high morbidity and mortality. For the treatment of gliomas, it is important to develop new and powerful treatments that could complement existing clinical treatment. Lonicera caprifolium L. (L. caprifolium) has various uses in herbal traditional medicine. This study was conducted to determine the phenolic acid levels and DNA damage protection potential of L. caprifolium extract, and to explore the antitumor effect of the extract by investigating its toxicity on C6 rat glioma cell lines and normal L929 mouse fibroblast cell lines. We also aimed to investigate the antiangiogenic potential of the extract. Method: Phenolic acid content was determined by HPLC analysis. DNA damage protection potential was evaluated on pBR322 plasmid DNA. The effect of extracts on the proliferation of cancer cells was evaluated by XTT assay. Antiangiogenic effect was determined with Chorioallantoic membrane model. Results: The extract was found rich in vanillic acid (273.003 µg/g); while the amount of chlorogenic acid was almost at negligible level (0.028 µg/g). 0.005-0.05 mg / ml extract protected against the hazardous effects of UV and H2O2 in all DNA bands. The presence of the extract significantly reduced C6 cell proliferation compared to control (p<0.05). The extract had antiproliferative effect with a half maximum inhibition of concentration (IC50) value of 0.45 mg/ml. L. caprifolium extract in 10 -6 , 10 -5 and 10 -4 M concentrations caused antiangiogenic effect. Antiangiogenic scores of L. caprifolium were 0.6, 0.73 and 1.6, respectively. Conclusions: These results show that L. caprifolium has potential cytotoxic and antiangiogenic effect on C6 rat glioma cells and that the phenolic acid content of the plant may partially influence these activities. Keywords: Glioma, Lonicera caprifolium, angiogenesis, cytotoxic effect, phenolic acid 7 CMJCumhuriyet Medical Journal ÖZET Amaç: Gliomolar yüksek morbitite ve mortalite oranı olan beyin tümörleridir. Gliomaların tedavisi için, mevcut klinik tedaviye tamamlayıcı olabilecek yeni ve güçlü tedavi çözümleri geliştirmek önemlidir. Lonicera caprifolium L.'nin (L. caprifolium) bitkisel geleneksel tıpta çeşitli kullanımla-rı vardır. Bu çalışma, Lonicera caprifolium L. (Bahçe Hanımelisi) ekstraktının fenolik asit düzey-lerini ve DNA hasarı koruma potansiyelini belirlemek ve C6 sıçan glioma hücre hatları ve normal L929 fare fibroblast hücre hatları üzerindeki toksisitesinin inceleyerek Lonicera caprifolium ekstraktının anti-tümör etkisini araştırmak için gerçekleştirilmiştir. Bu çalışmada ayrıca ekstraktın antianjiyogenik potansiyelinin araştırılması amaçlanmıştır. Yöntem: Ekstraktların fenolik asit içeriği, HPLC analizi ile tespit edildi. DNA hasarı koruma potansiyeli pBR322 plazmid DNA üzerinde değerlendirildi. Ekstraktın kanser hücrelerinin proliferasyonu üzerindeki etkisi XTT testi ile değerlendirildi. Anti-anjiyogenik etki koryoallonterik membran modeli ile tespit edildi. Bulgular: Ekstrakt vanillik asitten (273,003 µg/g), zengin bulundu; kl...

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In VitroAntibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates ofStenotrophomonas maltophiliaincluding the Trimethoprim/Sulfamethoxazole Resistant Strain

Cited by 101 publications

References 30 publications

Evaluation of phenolic compounds, antioxidant and antimicrobial activities from transgenic hairy root cultures of gherkin ( Cucumis anguria L.)

Evaluation of phenolic compounds, antioxidant and antimicrobial activities from transgenic hairy root cultures of gherkin ( Cucumis anguria L.)

Detection of caffeic and chlorogenic acids from methanolic extract of Annona squamosa bark by LC-ESI-MS/MS

Anticancer and antiangiogenic effects of methanol extracts of Lonicera caprifolium L. on C6 rat glioma cells

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