In vitro antileukaemic activity of extracts from chokeberry (Aronia melanocarpa [Michx] Elliott) and mulberry (Morus alba L.) leaves against sensitive and multidrug resistant HL60 cells

Abstract

The aim of the present study was to determine in vitro antileukaemic activities of extracts obtained from chokeberry (Aronia melanocarpa [Michx] Elliot) and mulberry (Morus alba L.) leaves against promyelocytic HL60 cell line and its multidrug resistant sublines exhibiting two different MDR phenotypes: HL60/VINC (overexpressing P-glycoprotein) and HL60/DOX (overexpressing MRP1 protein). It was found that the extracts from chokeberry and mulberry leaves were active against the sensitive leukaemic cell line HL60 and retained the in vitro activity against multidrug resistant sublines (HL60/VINC and HL60/DOX). The values of resistance factor (RF) found for these extracts were very low lying in the range 1.2-1.6.

Comparison of phenolic compounds and antioxidant potential between selected edible fruits and their leaves

“…It has been proven that chokeberry leaves (Aronia melanocarpa) extracts inhibit lipid and protein peroxidation in the brain of rats treated with oxidative stress-inducing factors (Cuvorova, Davydov, Prozorovskii, & Shvets, 2005). Skupień et al (2008) described antileukaemic activity of chokeberry leaves against human promyelocytes of several cell lines (HL60, HL60/ VINC and HL60/DOC), while Maslov et al (2002) in a rat study revealed their antidiabetic properties.…”

“…As some studies show, leaves of well-known crops and wild growing plants (such as blackcurrant, chokeberry and bilberry) are a valuable source of antioxidant substances, especially polyphenols (Skupień , Kostrzewa-Nowak, Oszmiań ski, & Tarasiuk, 2008;Tabart et al, 2007;Witzell, Gref, & Näsholm, 2003). It has been proven that chokeberry leaves (Aronia melanocarpa) extracts inhibit lipid and protein peroxidation in the brain of rats treated with oxidative stress-inducing factors (Cuvorova, Davydov, Prozorovskii, & Shvets, 2005).…”

Antioxidant Activity of Extracts from Apple, Chokeberry and Strawberry.

“…They are excellent as free radical scavengers including those in the form of the reactive forms of oxygen [Pajk et al, 2006;Skupień et al, 2008]. The radicals are the products of metabolic reactions within the living organism and are also formed in the external medium, e.g.…”

Morus rubra Extract Induces G1 Cell Cycle Arrest and Apoptosis in Human Lung and Prostate Cancer Cells

“…8 Also, Skupien et al reported that M. alba leaf extract has cytotoxic effect both human leukaemic cell line (HL60) and its multidrug resistant sublines. 31 Some researches now focus on the bioactive components of mulberry and concordantly Dat et al reported 11 flavonoids from the methanol extract of M. alba leaves, which were cytotoxic to HeLa, MCF-7, and Hep3B cancer cells. 32 Interestingly, synergistic cytotoxic effect of a natural compound may not observed when its purified polyphenolic compounds were used.…”