In vitroAntilisterial Effects of Citrus Oil Fractions in Combination with Organic Acids

Abstract

The objectives of this study were to screen activity of citrus essential oil fractions (EOs) alone and in combination with organic acids against 2 species of Listeria. Five citrus EOs were initially screened by disc diffusion assay for antibacterial activity. Cold pressed terpeneless Valencia orange oil (CP terpeneless oil) had the strongest bacteriostatic (MIC) and bactericidal (MBC) properties at 0.55% and 1.67%, respectively. Four organic acids were tested for effectiveness against Listeria. Citric and malic acids proved to be the most effective with MBC of 1.1% alone. Assays were conducted to determine synergistic effects of EOs and citric or malic acids. There was a significant decrease in MIC and MBC to 0.04% EO plus 0.12% malic or citric acid. EOs from citrus paired with organic acids offer the potential as an all-natural antimicrobial for improving the safety of all-natural foods.

Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

“…Synergism among antilisterial agents have been investigated to achieve higher levels of food safety standards (Kim et al 2008;Friedly et al 2009); but no report has been found or published in the literature on the synergistic effect of enterocin A and thyme EOs against L. monocytogenes . The exact mechanism of action of the essential oil and bacteriocins is not known, but some authors hypothesized that these components cause disruption of the cell membrane (Hyldgaard et al 2012).…”

Antimicrobial effects of combined UV-C or gamma radiation with natural antimicrobial formulations against Listeria monocytogenes, Escherichia coli O157: H7, and total yeasts/molds in fresh cut cauliflower

“…The concept of combining EOs and OAs has proven to be efficacious as an alternative to Antimicrobial Growth Promoters (AGP) in animal nutrition (Aksit, Goksoy, Kok, Ozdemir, & Ozdogan, 2006). Moreover, it has been reported that there was a significant synergistic antibacterial effect by combination of citrus oils and OAs against L. monocytogenes (Friedly et al, 2009). In previous experiments (Tawema, Han, Salmieri, Vu, & Lacroix, 2014), we developed two natural antimicrobial formulations which composed of lemongrass EO (for Asian formulation) or oregano EO (for Mediterranean formulation) plus citrus extract and lactic acid.…”

“…and Clostridium sp., Staphylococcus aureus), spoiling microorganisms (Lactobacillus sp. in juices), yeasts and molds (Saccharomyces cerevisiae, Aspergillus parasiticus, Aspergillus flavus, Alicyclobacillus acidoterrestris, Fusarium oxysporum) (Fisher & Phillips, 2008;Friedly et al, 2009;Bevilacqua, Campaniello, Sinigaglia, Ciccarone, & Corbo, 2012. Citral and carvacrol were used at different concentrations for controlling the growth of E. coli K12 and Listeria innocua at different temperature (Belda-Galbis, Pina-P erez, Leufv en, Martínez, & Rodrigo, 2013).…”

Antimicrobial activity of autoclaved and non autoclaved copaiba oil on Listeria monocytogenes

“…Study has showed a synergistic effect, against Listeria species, with the combination of terpenic fractions of citrus oil and organic acids as malic acid and ascorbic acid (FRIEDLY et al, 2009). The copaiba oil, which is composed by a sesquiterpenic and terpenic fractions, may has a similar action in combination with organic acids.…”