In vitro antimicrobial activities of Bidens pilosa and Moringa oleifera leaf extracts and their effects on ground beef quality during cold storage

Abstract

This study determined the activities of Bidens pilosa and Moringa oleifera leaf extracts on microbial count of ground beef during 6-day cold storage. Fresh ground beef sample was treated with butylated hydroxytoluene (BHT) (0.2 g/kg), M. oleifera (ML, 1 g/kg) and B. pilosa (BP, 1 g/kg) leaf extracts and compared with the control. The result of the phytochemical contents revealed that ML extract had higher phenolic and flavonoid contents than BP extract (p > 0.05). The antibacterial assay of the extracts revealed an appreciable broad-spectrum activity against tested bacteria with minimum inhibitory concentrations between 0.6 and 10.0 mg/mL. Addition of ML leaf extract to ground beef sample lowered total viable and lactic acid bacteria (p < 0.05) counts than control and BHT treatments at day 3 of storage. These results suggest that ML leaf extract could be used as potential sources of natural antimicrobial agent in meat products.Actividades antimicrobianas in vitro de extractos de hoja de Bidens pilosa y Moringa oleifera y sus efectos en la calidad de ternera triturada durante el almacenamiento en frío RESUMEN Este estudio determinó las actividades de los extractos de hoja de B. pilosa y M. oleifera en los recuentos microbianos de ternera triturada durante 6 días de almacenamiento en frío. Se trató con BHT una muestra fresca de ternera triturada (0,2 g/kg) y extractos de hoja M. oleifera (ML, 1 g/kg) y B. pilosa (BP, 1 g/kg), entonces estos fueron contrastados con la muestra control. Los resultados de los contenidos fitoquímicos revelaron que el extracto de ML tenía un mayor contenido fenólico y de flavonoides en comparación con el extracto de BP (p > 0,05). El ensayo antibacteriano de los extractos reveló una apreciable actividad de amplio espectro frente a las bacterias examinadas con concentraciones inhibitorias mínimas utilizando rangos entre 0,6 y 10,0 mg/mL. La adición de extracto de hoja de ML a la muestra de ternera triturada disminuyó el recuento total viable de bacteria ácido-láctica (p < 0,05) más que en la muestra control y los tratamientos de BHT en tercer día de almacenamiento. Estos resultados sugieren que el extracto de hoja de ML podría ser utilizado como fuente potencial de agente antimicrobiano natural en los productos cárnicos. ARTICLE HISTORY

Lactation performance and blood metabolites in lactating dairy cows micro-supplemented with <i>Moringa oleifera</i> leaf meal

“…Moringa leaf powder contains high levels of flavonoids, vitamin E, A and selenium, which could have contributed to the observed high antioxidant activity in the current study synergistically, as reported elsewhere (Falowo et al, 2016). Babiker et al (2017) and Gopalakrishnam et al (2016) reported that positive Moringa effects on milk yield were associated with a higher intake of the plant.…”

“…In addition, M. oleifera leaf meal contains phenolic and flavonoids at 31.9 g/100g and 40.8 g/100g, respectively (Pakade et al, 2013). These compounds have antioxidative and immunomodulatory properties (Moyo, 2011) that are more effective than synthetic antioxidants such as butylated hydroxytoluene, rutin, and ascorbic acid as noted by Falowo et al, (2016). Because early lactating dairy cows are prone to oxidative stress, M. oleifera may serve as a natural and cheaper antioxidant source in resource limited-areas.…”

Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extracts

“…Nonetheless, in the current reaserch, the T. trifidum extracts showed activity against gram-negative and gram-positive bacteria. A related study, but using extracts from Bidens pilosa and Moringa oleifera ( Falowo et al., 2016 ), also showed potency against gram-negative bacteria. It is noteworthy that like the T. trifidum extracts, the B. pilosa and M. oleifera extracts, also contained phytochemicals with antibacterial activity.…”

Antioxidant activities of Moringa oleifera L. and Bidens pilosa L. leaf extracts and their effects on oxidative stability of ground raw beef during refrigeration storage

“…The dried powder of plant extracts were then stored at 20°C for further analysis. The phenolic contents of the extracts have been analyzed previously (Falowo et al, 2016).…”

“…Reports on their nutritional contents have also showed that they are rich in proteins (including essential amino acids), vitamins, beta-carotene, minerals and low in fat and carbohydrates (Adedapo et al, 2011;Moyo et al, 2012;Bartolome et al, 2013). The antioxidant and biological activities of these plants have been attributed to the presence of phytochemicals including flavonoids and other phenolics in their leaves extract (Al-Owaisi, Al-Hadiwi, & Khan, 2014;Falowo, Muchenje, Hugo, & Charimba, 2016).…”