In vitroantimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite

Abstract

The high number of biomaterial associated infections demands new strategies to prevent this problem. In this study the suitability of nanohydroxyapatite (nanoHA)-based surfaces containing two Brazilian extracts of propolis (green and red ones) to prevent bacterial growth and biofilm formation, as well as its non-cytotoxic nature, was investigated. Optical density, colony forming units and MTT reduction assay were used to assess the materials' antibacterial activity against planktonic and sessile growth of Staphylococcus aureus. NanoHA matrix was able to absorb both types of propolis and the obtained results revealed the antibacterial effectiveness of the novel materials expressed as the reduction of bacterial growth and biofilm formation ability. Additionally, cell culture tests showed the growth of fibroblasts with high metabolic activity and without membrane damage. Therefore, these nanoHA-based surfaces containing natural products deriving from bees may be a promising bioactive biomaterial to be further studied with the aim of application to orthopaedic or dental devices.

Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian

“…Its biological activity is determined mainly by compounds from the polyphenolic fraction, especially flavonoids, followed by aromatic acids, phenol acid esters, triterpenes, lignans, etc. (Celikel & Kavas 2008;Popova et al 2014;Grenho et al 2015;Machado et al 2016).…”

Mesoporous bioactive glass embedding propolis and cranberry antibiofilm compounds

“…(Naturex-DBSV R , MA) were diluted in a 50% (v/v) hydro-alcoholic solution (EtOH, P.A., Synth, Brazil) under magnetic stirring at room temperature for 5 h to prepare solutions of 5 and 10 lg/mL as described in a previous study. 31 Then, 0.5 g of MBG powder was immersed into 50 mL of the corresponding solutions with different concentrations and stirred at 378C for 24 h. MBG powders were also added into a hydroalcoholic solution free of natural derived compounds to prepare the control group. After immersion, the solutions containing MBG powders were centrifuged and dried in a vacuum oven at 408C for 3 h.…”

“…For example, surface functionalization of BGs has been explored for the incorporation of natural biomolecules such as polyphenols, which have potential antibacterial benefits . Additionally, a herbal derived substance called propolis, which is a resinous natural substance collected by bees, has being described to have several health promoting properties like fibroblast stimulation, and antioxidant, antifungal, antiviral and antibiofilm activities . Propolis has shown noncytotoxic effects, but it has not been deeply explored in tissue engineering applications .…”

“…30 Additionally, a herbal derived substance called propolis, which is a resinous natural substance collected by bees, has being described to have several health promoting properties like fibroblast stimulation, and antioxidant, antifungal, antiviral and antibiofilm activities. 29,31 Propolis has shown noncytotoxic effects, but it has not been deeply explored in tissue engineering applications. 32,33 Only one study 29 has incorporated red and green propolis collected from different regions into BG, showing a satisfactory growth inhibition of S. aureus, E. faecalis, S. mutans, P. intermedia, F. nucleatum, P. gingivalis, and, A. actinomycetemcomitans.…”

Efficacy of different extracts of propolis against Salmonella enterica serovar Typhimurium: In vitro and in vivo study

“…It is mainly composed of resin and vegetable balsam (50%), wax (30%), essential and aromatic oils (10%), pollen (5%) and various other substances including organic compounds and minerals (5%) (Tylowski et al, 2006;Kaur et al, 2013). Propolis has been used in folk medicines in many regions of the world and has been reported to have various biological activities, such as antibacterial ( Grenho et al, 2015), antiinflammatory (Chen et al, 2004) antitumor effects (Watanabe et al, 2011 andHasan et al, 2014) ) and immunomodulatory effects (Sforcin, 2007). There are a lot of studies favoring the use of different biologically active natural products for the treatment of serious ailments are being emphasized.…”