In vitro antioxidant and anti-aging properties of swim bladder peptides from Atlantic cod (Gadus morhua)

Abstract

Two bioactive peptide fractions (SWP-I and SWP-II) of swim bladder peptides from Atlantic cod (Gadus morhua) were obtained through enzymatic hydrolysis and isolated by gel filtration chromatography. The properties of two fractions were characterized by FT-IR, UV scanning spectroscopy, amino acid composition analysis and molecular weight determination. The results showed that the content of aromatic amino acids in SWP-II was higher than that in SWP-I, and the molecular of SWP-I and SWP-II were 4976 Da and 1960 Da, respectively. The radical scavenging activities showed that SWP-I and SWP-II were effective scavengers of DPPH•, HO•, and O 2-•, and demonstrated high Fe 2+-chelating activity. The scavenging capacities of SWP-II were stronger than those of SWP-I at the same concentration. Cytoprotective experiment suggested that SWP-I and SWP-II possessed ROSscavenging activity. Pretreatment with these two peptides increased viability rates, suppressed SA-β-gal activities and inhibited apoptosis rates in hydrogen peroxide (H 2 O 2)-induced premature senescent 2BS cells. In conclusion, the enzymatic peptides from swim bladders of Atlantic cod (Gadus morhua) possessed antioxidant activities and could effectively protect premature cell senescence induced by H 2 O 2 .

Swim bladder-derived biomaterials: structures, compositions, properties, modifications, and biomedical applications

“…Collagen yield varies from 30 to 60% based on the species and methods of isolation [ 31 , 70 , 71 ]. Owing to the presence of specific peptides and amino acids, fish collagen hydrolysates have pharmacological benefits, such as anti-inflammatory, antibacterial, antitumor, antiaging, and free radical scavenging activities [ 72 , 73 ].…”

Potential Active Marine Peptides as Anti-Aging Drugs or Drug Candidates

“…The results showed that CSSPs played an anti-oxidant and anti-aging role [ 48 ]. Moreover, two bioactive peptide fractions (SWP-I and SWP-II) from the swim bladder of Atlantic cod (Gadus morhua) were isolated and pretreated with SWP-I and SWP-II on prematurely senescent 2BS cells induced by hydrogen peroxide (H 2 O 2 ), and it was found that SWP-I and SWP-II could effectively scavenge DPPH-, HO - , and O 2− ; improve Fe 2+ chelating activity; increase cell survival; and inhibit SA-β-gal activity and apoptosis rate, indicating that they are peptides with both anti-oxidant and anti-aging properties [ 49 ]. There are also studies on grass carp (Ctenopharyngodon idella) skin, where three novel peptides (PTSPL/GPGPGL/VGGAP) with known sequences derived from the proteolytic digest of grass carp skin have potent anti-oxidant activities, and although their potential health benefits need to be explored through simulated digestion and animal studies, the powerful anti-oxidant activities shown in the current study and the findings provide evidence that these three anti-oxidant peptides are useful in delaying aging or extending the life of cells.…”

Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells

“…The absorbance was detected at 280 nm. According to the elution curve, the main fractions were collected as AKP (Li et al, 2020).…”

“…can react with almost every biological molecule found in living cells and cause tissue damage through oxidizing proteins, carbohydrates, nucleic acids and lipids. DPPH• is another strong reactive free radical which is stable in ethanolic solutions and has a maximum absorbance at 517 nm because of its odd electron ion (Li et al, 2020).…”