In vitro antioxidant, hypoglycemic and oral glucose tolerance test of banana peels

Abstract

Banana fruit is claimed to have antidiabetic effects despite its high calorie content, and its peels also contain vital phytoconstituents including gallocatechin. Previously banana pulp has been studied for antihyperglycemic effects, and in the present investigation antihyperglycemic effect of ethanolic extract of inner peels of Musa sapientum (EMS), Musa paradisiaca (EMP), Musa cavendish (EMC) and Musa acuminata (EMA) fruit was evaluated using oral glucose tolerance test in normoglycemic rats. In vitro antioxidant study was conducted using DPPH, H 2 O 2 radical scavenging assay and ferric reducing power assay. Wistar rats were divided into fourteen groups and twelve groups received different doses of aforementioned extracts, while control group received gum acacia solution and remaining group received standard drug, glimepiride. All the rats received glucose load at a dose of 2 g/kg body weight. Groups treated with EMC and EMA showed significant decrease in glucose level (p < 0.01) at 150 min as compared to control group. In hypoglycemic study, only EMP 500 mg/kg, p.o. treated group revealed a significant decrease (p < 0.05) in glucose level at 120 min, while other groups did not show any sign of hypoglycemia. In glucose tolerance test, animals treated with EMC and EMA depicted dose dependent antihyperglycemic effect at 150 min while EMS and EMP showed significant reduction in plasma glucose at higher doses. In a similar fashion, EMA i.e. M. acuminata demonstrated highest antioxidant activity followed by EMC against DPPH radical. In ferric reducing power and H 2 O 2 scavenging assay, EMA demonstrated maximal antioxidant activity when compared with other extracts.

Bioactive screening and in vitro antioxidant assessment of Nauclea latifolia leaf decoction

“…Donating electron to reduce ferric ion to ferrous ion and Mo (VI) to Mo (V) is an indication of reducing power. The reducing capacity of a compound may be directly translated as an indicator of antioxidant activity [14]. The strong reducing power of N. latifolia ethanolic leaf extract might be as a result of its phenolic content, as they are good electron donors [15].…”

Pharmacological Activities of Banana

“…In ferric reducing power and H 2 O 2 scavenging assay, Musa acuminata also showed best antioxidant activity when compared with other extracts. The study revealed that the peels of Musa species possess significant in vitro antioxidant activity, hence, the conclusion that eating the peel of banana fruit would be beneficial considering its potential antioxidant property [24]. It was also reported that ethanol extracts of unripe Cavendish (Musa acuminata L) and Dream banana (Musa acuminata colla.…”

“…High level of glucose is prominent in diabetic patients and attempts have been made to counteract that using several plants including banana. Investigation into the anti-hyperglycemic effect of ethanol extract of Musa sapientum (EMS), M. paradisiaca (EMP), Musa cavendish (EMC), and M. acuminata (EMA) peels by oral glucose tolerance test in glucose-loaded (2 g/kg p.o) normoglycemic (having normal concentration of glucose) rats was done by [24]. It was observed from this study that animals treated with EMC (500 and 1000 mg/ kg, p.o) and EMA (200 and 400 mg/kg p.o) showed marked anti-hyperglycemic effect (p < 0.01).…”

An overview of anti-diabetic plants used in Gabon: Pharmacology and toxicology

“…Ethanolic extract of banana peels at 500 mg/kg exhibited minor reduction of blood glucose in normal rats compared to normal, 120 min after extract administration. Also, the same dose showed antihyperglycemic effects compared to normal during OGTT and from 90 min (Navghare and Dhawale, 2017) Hepatoprotective, Antioxidant (Swathi et al, 2011) Myrtaceae: Psidium guajava L. (Mathur et al, 2015).…”