In vitro assessment of dual‐network electrospun tubes from poly(1,4 cyclohexane dimethylene isosorbide terephthalate)/PVA hydrogel for blood vessel application

Abstract

The fabrication of artificial blood vessel remains an ongoing challenge for cardiovascular tissue engineering. Full biocompatibility, proper physiological, and immediate availability have emerged as central issues. To address these issues, the dual-network composite scaffolds were fabricated by coating the electrospun nanofibers-based tubes with poly(vinyl alcohol) (PVA) hydrogel, which could increase the cell viability and show the potential for controlling the composition, structure, and mechanical properties of scaffolds. Herein, the tubular scaffolds having an inner diameter of 2 mm, were composed with poly(1,4 cyclohexane dimethylene isosorbide terephthalate)/PVA. The morphology examination showed that tubular structure was dimensionally stable and suitable for an artificial blood vessel. Fourier transform infrared spectra, wetting behavior, stress-strain behavior, and Thiazolyl Blue Tetrazolium Bromide (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) analysis also showed that the composite scaffolds have good chemical interactions between poly(1,4 cyclohexane dimethylene isosorbide terephthalate) (PICT) and PVA, blended PICT/PVA tubes showed the appropriate wetting behavior, it achieved the appropriate breaking strength and adequate pliability up to 47.5% and in vitro assessment showed that blended PICT/PVA scaffolds have the appropriate cell viability and nontoxic, respectively. On the basis of characterizations results, it was concluded that resultant scaffolds would be addressed to fulfill the requirements such as biocompatibility, dimensional stability, adequate elongation, breaking strength, immediate availability, and proper for physiologically.

<p>Antibacterial properties of in situ and surface functionalized impregnation of silver sulfadiazine in polyacrylonitrile nanofiber mats</p>

“…Natural and synthetic fibers have been electrospun either in single form or blends of different types of polymers to obtain desired features. 9,[33][34][35][36][37][38][39] Figure 1 briefly illustrates the process of electrospinning.…”

In-vitro assessment of appropriate hydrophilic scaffolds by co-electrospinning of poly(1,4 cyclohexane isosorbide terephthalate)/polyvinyl alcohol

“…To measure the dye absorption by the scaffolds, all samples without any cells were immersed in a medium culture with the addition of MTT, then washed with DMSO and absorbance was read at 570 nm. For the estimation of the number of cells adhering to the scaffolds, the absorbance of scaffolds was subtracted from the absorbance in the presence of cell line L929 26 .…”

“…Both types of nanofibers have different properties of stress strain behavior, but their blend showed the different behavior from them due to the interlacement of high elongated PVA nanofibers and high stiffed PICT nanofibers 24,25 . The behavior of PICT/ PVA nanofibers is appropriate for the scaffold application because there is need of appropriate elongation and stiffness which was obtained by co-electrospinning of PVA and PICT polymers 26 .…”

Fabrication and characterization of wound dressings containing gentamicin/silver for wounds in diabetes mellitus patients

“…Traditional wound dressings are mainly dry gauze and oil gauze, which cannot be used for infectious wounds, and have no promoting effect on wound healing, so they cannot be used for wounds of diabetics. The bioactive dressings are made of polymer materials and biological materials processed by high-tech methods, which is a hot spot in the research and development of wound dressings [7,8]. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.…”