In vitro biological response to a self‐adhesive resin cement under different curing strategies

Abstract

Self-adhesive resin cements represents a new approach in indirect restorative dentistry, although little is known about its biological properties. The influence of the curing strategy on the biological response of a self-adhesive resin cement (Rely-X Unicem/3M ESPE) was evaluated through methyltetrazolium (MTT) and nitric oxide (NOx) assays. Cylindrical specimens (n = 48) were prepared and cured according to the following strategies: photoactivation with halogen light-curing unit (LCU) Demetron/Kerr, LED LCU Radii/SDI, and chemical activation. A control group with culture medium and a group with lipopolysaccharide, employed for cells stimulation, were used for comparisons (n = 8). After 24 h of direct contact between specimens and rat alveolar macrophages, the biological response was evaluated. Statistical evaluation of the data was performed with Kruskal-Wallis and Dunn's test (p = 0.05). The MTT test showed that the specimens cured by halogen light and chemical activation provided higher alterations on cell metabolism. For LED-cured specimens, cell viability was compatible to the results observed in the control group. For the NOx assay, all curing methods were cytotoxic to rat macrophages. Rely-X Unicem demonstrated different cytotoxic effects according to the curing strategy employed.

Titanium dioxide nanotubes addition to self-adhesive resin cement: Effect on physical and biological properties

“…The increase of DC values of the reinforced cement influenced most of the other properties studied, as found in other studies [37,38,48]. In a general analysis, the addition of TiO 2 -nt in the evaluated self-adhesive resin cement positively influenced its properties, especially in the self-curing mode, which has an indication for use with certain limitations.…”

“…However, the DCT group at 24 h showed a significant difference in cell viability when compared to CP. This result can be explained by the relationship between the type of polymerization employed and the DC obtained with regard to the cytotoxicity of resin cements [37]. The cements with lower DC showed a significant decrease in cell survival when compared to cements with the highest DC [38].…”

In vivo evaluation of inflammation and matrix metalloproteinase expression in dental pulp induced by luting agents in dogs

“…14 Several variables modulate RU cytotoxicity, depending, among other factors, on the curing strategy. 9 In the present study, the compatibility of RU after indirect pulp capping in deep cavities demonstrates that the remaining dentin, even if it is thin, can act as a barrier against the diffusion of possibly harmful chemical components in the material, thus avoiding direct contact of these components with the underlying connective pulp tissue and attenuating possible toxic effects. 29 Our findings support a previous in vitro investigation that used dentin disks to investigate the cytotoxicity of RU and found that more than 80% of cells remain viable after 24 hours or 7 days of indirect contact with the cement.…”

“…5 The mechanical, chemical and biological properties of KC and RU have been investigated previously. 4,6,7,8,9,10,11,12,13,14,15,16 Because most teeth that require prosthetic treatment exhibit extensive coronal damage, investigations of the biological tissue response induced by luting agents on teeth with pulp vitality are relevant to understand the mechanisms of inflammatory reactions.…”

Resin luting materials: Tissue response in dog's teeth

“…These self‐adhesive cements have good moisture tolerance, release fluoride in a similar manner as glass ionomer cements (Radovic et al, ), do not require pretreatment of dentin with acid agents, and has been placed on deep dentin without producing an adverse pulpal response (Costa et al, ). Although a number of studies have evaluated the physicochemical and mechanical properties (Ernst et al, ; Escribano and De la Macorra, ; Holderegger et al, ) as well as the in‐vitro cytotoxicity (Bakopoulou et al, ; Monteiro et al, ; Ulker and Sengun, ) of the self‐adhesive resin cement Rely X™ Unicem, studies on the in‐vivo tissue response to this material are scarce (Costa et al, ) and further investigation is needed.…”