In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO₄:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cells

Abstract

Highly dispersible Eu3+-doped CaMoO4@Au-nanorod hybrid nanoparticles (HNPs) exhibit optical properties, such as plasmon resonances in the near-infrared region at 790 nm and luminescence at 615 nm, offering multimodal capabilities: fluorescence imaging, surface-enhanced Raman spectroscopy (SERS) detection and photothermal therapy (PTT). HNPs were conjugated with a Raman reporter (4-mercaptobenzoic acid), showing a desired SERS signal (enhancement factor 5.0 × 105). The HNPs have a heat conversion efficiency of 25.6%, and a hyperthermia temperature of 42°C could be achieved by adjusting either concentration of HNPs, or laser power, or irradiation time. HNPs were modified with antibody specific to cancer biomarker epidermal growth factor receptor, then applied to human lung cancer (A549) and mouse hepatocyte cells (AML12), and in vitro PTT effect was studied. In addition, the biomechanical properties of A549 cells were quantified using atomic force microscopy. This study shows the potential applications of these HNPs in fluorescence imaging, SERS detection, and PTT with good photostability and biocompatibility.

Photothermal Cellulose-Patch with Gold-Spiked Silica Microrods Based on Escherichia coli

“…The cell culturing of E. coli followed the reported protocol, 5 and the silica layer was coated on the E. coli by a modified Stöber method. 19 Then, 10 mL of culturing solution containing E. coli was dispersed in 50 mL of 80 v/v % ethanol solution, followed by sequentially adding 1 mL of NH 4 OH and tetraethyl orthosilicate solution dropwise. The mixture was stirred for 6 h, and the resulting particles were collected by centrifugation and washed three times with ethanol.…”

A multi-scale approach to study biochemical and biophysical aspects of resveratrol on diesel exhaust particle-human primary lung cell interaction

“…Raman spectral peaks derived from human lung cells have been reported previously 47,48 that allow monitoring of DNA, proteins, and lipids changes over time. For example, most cells present a characteristic Raman peak at 1006 cm −1 assigning the presence of phenylalanine 49,50 , which was selected as the spectral indicator to monitor cell behavior 51 . In the DEP group, phenylalanine peak intensity decreased significantly after 4 h DEP exposure, and dropped from 0.97 at 0 h to 0.35 at 16 h (Fig.…”

Spatiotemporal dynamic monitoring of fatty acid–receptor interaction on single living cells by multiplexed Raman imaging

“…Most mammalian cells have a characteristic Raman peak around 1,002 cm −1 assigning the presence of phenylalanine (50,52), which has been selected as a sensitive marker for monitoring cellular protein structure (53). With a small spectral scan window (994 to 1,345 cm −1 ), it covers all three well-defined peaks of interest, including the cell characteristic peak (1,002 cm −1 ), GPR120 (1,078 cm −1 from MBA), and CD36 (1,328 cm −1 from DTNB).…”