<i>In vitro</i> breeding of high‐linolenic, doubled‐haploid lines of linseed (<i>Linum usitatissimum</i> L.) via androgenesis

Friedt, W.; Bickert, C.; Schaub, H.

doi:10.1111/j.1439-0523.1995.tb01242.x

Cited by 35 publications

(16 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In most cases, the percentage of regenerants ranged from 0.32% (Tejklova 1996) to less than 4% (Nichterlein 1991;Friedt 1995), while in cultivars selected for the present investigation, high regeneration frequency was detected. However, this result is as yet, not fully independent of the genotype.…”

Section: Discussionmentioning

confidence: 71%

Regeneration of flax ( Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to Fusarium oxysporum

et al. 2003

View full text Add to dashboard Cite

The aim of this study was to establish a protocol for the efficient production of flax plants of microspore origin. The results were compared to those obtained for plants regenerated from somatic explants from hypocotyls, cotyledons, leaves, stems and roots. All the plants obtained during the experiments were regenerated from callus that was grown for periods from a few weeks to a few months before the regeneration was achieved. Anther cultures were less effective in plant regeneration than somatic cell cultures. However, regenerants derived from anther cells showed valuable breeding features, including increased resistance to fungal wilt. The age of the donor plants and the season they grew in had a noticeable effect on their anther callusing and subsequent plant regeneration. Low temperature had a negative effect and dark pre-treatment a positive effect on callusing and plant regeneration. Different media were most effective for callus induction, shoot induction and rooting. For callus induction two carbon sources (2.5% sucrose and 2.5% glucose) were most effective; for shoots, only sucrose at lower concentration (2%) was effective. Rooting was most efficient in 1% sucrose and reduced (50%) mineral concentration in the medium. It was found that the length of in vitro cultivation significantly increases the ploidy and affects such features as regenerant morphological characteristics, petal colour, and resistance to Fusarium oxysporum-induced fungal wilt. The established plant regeneration system provides a basis for the creation of transgenic flax.

show abstract

Section: Discussionmentioning

confidence: 71%

Regeneration of flax ( Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to Fusarium oxysporum

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Rapid breeding techniques could help in producing new linseed lines such as those resistant to pathogen infection and/or with increased productivity (Rutkowska-Krause et al 2003). Haploidy has long attracted the interest of linseed breeders and geneticists because breeding linseed using haploidy techniques has the advantages of rapid development of completely homozygous lines and efficient means of genotypic selection (Friedt et al 1995). In addition, doubled haploid populations are ideal for genetic research, such as genetic segregation analysis and genome mapping (Chen et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Effect of genotype and medium composition on linseed (Linum usitatissimum) ovary culture

Burbulis¹,

Blinstrubienė²,

Kuprienė³

2011

Biologia

View full text Add to dashboard Cite

Breeding linseed (Linum usitatissimum L.) using haploid techniques allows breeders to develop new cultivars in a shorter time period. Many research groups successfully created new linseed genotypes through anther culture; however ovary culture has been the subject of only a few earlier studies. In the present study, the effect of genotype and growth regulators combination on callus induction and shoots regeneration in ovary culture of nine commercially important linseed cultivars was investigated. Ovaries were cultured on modified MS medium supplemented with three different combinations of plant growth regulators. Variable callogenic responses were expressed by all of the genotypes tested on different induction media. The results suggested that specific combination of growth regulators for callus induction must be designed for each genotype. Shoot regeneration from ovary derived callus is a critical phase of the whole gynogenetic process. Differences in adventitious shoot formation frequency among genotypes were demonstrated and four responsive genotypes have been selected. Ovary derived callus from cultivar 'Mikael' manifested the highest adventitious shoot formation frequency with a high number of shoots per explant. The optimum ratio of growth regulators for shoot regeneration was shown to depend on the genotype. Cultivars 'Linola', 'Mikael' and 'Szaphir' showed the highest shoot regeneration frequency when callus had originated on induction medium supplemented with 2 mg L −1 BAP and 2 mg L −1 NAA, while combination of 1 mg L −1 BAP and 2 mg L −1 IAA promoted shoot formation in ovary-derived callus of 'Barbara'. The highest rate of shoots per explant has been obtained in second subculture.

show abstract

“…Flax is the leading source of plant-based omega-3 FAs (Green 1986;Rowland 1991;Friedt et al 1995;Kenaschuk 2005), but there are at least three broad categories of genotypes with regard to LIN content. Conventional linseed varieties usually have 55-57 % LIN.…”

Section: Discussionmentioning

confidence: 99%

“…Linseed is the richest crop source of omega (ω)-3 fatty acids (FAs) praised for their health benefits in humans and animals. Conventional linseed varieties contain 55 to 57 % α-linolenic acid (ALA or LIN, C18:3 cis Δ9,12,15 ), but genetic variation for this trait exists with Solin™ or Linola™ lines having less than 5 % ALA and high LIN lines exceeding 65 % (Green 1986;Rowland 1991;Friedt et al 1995;Kenaschuk 2005).…”

Section: Introductionmentioning

confidence: 99%

Structural organization of fatty acid desaturase loci in linseed lines with contrasting linolenic acid contents

Thambugala

Ragupathy

Cloutier

2016

Funct Integr Genomics

View full text Add to dashboard Cite

Flax (Linum usitatissimum L.), the richest crop source of omega-3 fatty acids (FAs), is a diploid plant with an estimated genome size of ~370 Mb and is well suited for studying genomic organization of agronomically important traits. In this study, 12 bacterial artificial chromosome clones harbouring the six FA desaturase loci sad1, sad2, fad2a, fad2b, fad3a and fad3b from the conventional variety CDC Bethune and the high linolenic acid line M5791 were sequenced, analysed and compared to determine the structural organization of these loci and to gain insights into the genetic mechanisms underlying FA composition in flax. With one gene every 3.2-4.6 kb, the desaturase loci have a higher gene density than the genome's average of one gene per 7.8-8.2 kb. The gene order and orientation across the two genotypes were generally conserved with the exception of the sad1 locus that was predicted to have additional genes in CDC Bethune. High sequence conservation in both genic and intergenic regions of the sad and fad2b loci contrasted with the significant level of variation of the fad2a and fad3 loci, with SNPs being the most frequently observed mutation type. The fad2a locus had 297 SNPs and 36 indels over ~95 kb contrasting with the fad2b locus that had a mere seven SNPs and four indels in ~110 kb. Annotation of the gene-rich loci revealed other genes of known role in lipid or carbohydrate metabolic/catabolic pathways. The organization of the fad2b locus was particularly complex with seven copies of the fad2b gene in both genotypes. The presence of Gypsy, Copia, MITE, Mutator, hAT and other novel repeat elements at the desaturase loci was similar to that of the whole genome. This structural genomic analysis provided some insights into the genomic organization and composition of the main desaturase loci of linseed and of their complex evolution through both tandem and whole genome duplications.

show abstract

In vitro breeding of high‐linolenic, doubled‐haploid lines of linseed (Linum usitatissimum L.) via androgenesis

Cited by 35 publications

References 25 publications

Regeneration of flax ( Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to Fusarium oxysporum

Regeneration of flax ( Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to Fusarium oxysporum

Effect of genotype and medium composition on linseed (Linum usitatissimum) ovary culture

Structural organization of fatty acid desaturase loci in linseed lines with contrasting linolenic acid contents

Contact Info

Product

Resources

About