In vitro cell death induced by irradiation and chemicals relevant for dental applications; dose–response and potentiation effects

Abstract

Resin-based dental materials polymerized using blue light are frequently used in dental practice and may come in contact with the oral mucosa. Remnants from oral hygiene product ingredients, such as sodium lauryl sulfate (SLS), add to the chemical exposure of the mucosa. The aim of the present in vitro study was to elucidate the cytotoxic effects in terms of apoptosis and necrosis after exposures to combinations of an adhesive (0.5% and 0.6%), SLS (concentration range 0.0025%-0.0075%), and irradiation from a dental curing lamp (radiant exposure of 8 J cm(-2)). The test system chosen was rat submandibular salivary gland acinar cells, and the cytotoxic effects were measured by fluorescence microscopy and flow cytometry methods. Cytotoxicity was observed as a result of irradiation. The most pronounced cytotoxic effects were seen in cells exposed to a combination of adhesive and SLS compared with those exposed to either agent alone. Necrosis was the dominating form of cell death for all exposures, except for the highest concentration of SLS. Apoptosis was dose-dependent on SLS in the rat submandibular acinar cells. Cytotoxic considerations of dental materials should include contributions from irradiation and other chemicals that might be present in the oral cavity.

Effect of N-acetyl-l-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts

“…The concentration range of HEMA in our study was in line with previous in vitro investigations on dental resin monomers [6,10,[21][22][23][24], although the effective HEMA concentrations which may reach in oral and pulp tissues are unknown until now. In some clinical trials, the toxic concentrations will depend on the procedures used (e.g.…”

Apototic and Necrotic Influence of Dental Resin Polymerization Initiators in Human Gingival Fibroblast Cultures

“…In the presence of calcium ions, annexin V binds specifically to PS 31) . The externalization of PS is an important cell surface signal for the macrophage clearance of apoptotic cells 32) . Recent reports have shown that resin-based dental materials caused cell death by apoptosis and necrosis in rat submandibular acinar cells and human endothelial cells 32,33) .…”

“…The externalization of PS is an important cell surface signal for the macrophage clearance of apoptotic cells 32) . Recent reports have shown that resin-based dental materials caused cell death by apoptosis and necrosis in rat submandibular acinar cells and human endothelial cells 32,33) . The resin monomer Bis-GMA and comonomer TEGDMA augment apoptosis in dose-and time-dependent manners in HGF cells 34,35) .…”

Effects of visible light-irradiated camphorquinone and 9-fluorenone on murine oral mucosa

“…Therefore, we presumed that the test agents infiltrated but did not destroy the corium, although they affected the architecture of the prickle cell layer. CQ induced a time-and dose-dependent increase in the rates of apoptosis and necrosis in cell lines, and the same could be said when coupled with VL irradiation 14) . In this study, we recognized a few TUNEL-positive nuclei after CQ application.…”