<i>In Vitro</i>Culture of Wheat and Genetic Transformation — Retrospect and Prospect

Maheshwari, N.; Rajyalakshmi, K; Baweja, K.; Dhir, Sarwan K.; Chowdhry, C. N.; Maheshwari, S. C.

doi:10.1080/07352689509701925

Cited by 13 publications

(5 citation statements)

References 222 publications

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“…Therefore, DH lines have the potential for use in plant breeding, mutagenesis, in vitro selection, molecular mapping and plant transformation . To confirm the advantages of DH lines in plant breeding, genetic stability of DH progenies has been studied widely in the past 20 yr as reviewed in wheat De Buyser and Henry 1986;Hu and Yang 1986;Picard 1990;Maheshwari et al 1995), barley (Choo et al 1985Pickering and Devaux 1992), rice (Chen 1986;Alejar et al 1995) and corn (Wu 1986;Genovesi 1990). Results indicate that about 90% of the doubled lines were genetically uniform.…”

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confidence: 99%

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Hu¹,

Kasha²

1997

Can. J. Plant Sci.

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. 1997. Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.). Can. J. Plant Sci. 77: 549-554. Ploidy level, genetic stability and field performance of isolated microspore-derived wheat plants were evaluated. The ploidy levels of isolated microspore-derived wheat plants from cv. Chris and reciprocal crosses of Chris × Sinton were determined by two methods, namely chromosome counts of root tip cells and flow cytometric measurement of nuclear DNA content from leaves. Both methods gave similar results with the frequencies of spontaneous chromosome doubling and completely fertile plants among microspore-derived H 0 plants of wheat being about 80% and 75%, respectively, based on two H 0 populations. Only 1.7% were aneuploid and 16% were haploid. Spontaneous mutation frequencies were low with 1 of 124 Chris DH plants having a recessive mutant for lemma awns while three were grass-like plants in the H 0 generation. The field-measured traits of microspore-derived DH lines were similar to the check by the third generation. Ninety-two percent and 70% of Chris DH lines had 1000-kernel weights and yields similar to the check, respectively. These results indicate that microspore-derived DH lines should have good potential for applications in plant breeding, in vitro selection, plant transformation and genetic studies.

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confidence: 99%

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Hu¹,

Kasha²

1997

Can. J. Plant Sci.

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“…This indicates that this method is almost independent of the genotypic influence (see also Parihar et al, 1994) probably due to the underlying biochemical/biophysical principle involved during cellular permeabilization. It also offers a relative advantage over the other techniques, which have limited utility for recalcitrant genotypes (Tyagi et al, 1989;DeBlock and DeBrouwer, 1991;Maheshwari et al, 1995). Although biolistics appear to be the method of choice for cereal transformation (Hansen and Wright, 1999), cellular permeabilization offers various advantages.…”

Section: Genotypic Effectmentioning

confidence: 99%

Exogenous DNA Uptake via Cellular Permeabilization and Expression of Foreign Gene in Wheat Zygotic Embryos.

Mahalakshmi

Chugh

Khurana

2000

Plant Biotechnology

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Exogenous DNA uptake and transient expression of the GUS reporter gene was studied in wheat (Triticum aestivum L.) zygotic embryos employing a simple procedure of cellular permeabilization by membrane interactive agents like saponin and toluene. Uptake of expression vectors with different promoters was detected in mature and immature embryos. The frequency of GUS expression was detected to be higher in mature embryos than immature embryos probably due to a certain degree of disorganization of the plasmalemma during the dehydrated state of the embryos. Of the various permeabilizing agents employed, saponin and toluene were detected as potential membrane permeabilizers without adversely affecting embryo viability. Variations in GUS gene expression were not significant among different genotypes of T aestivum CPAN1676, PBW343 and HD2329, thus indicative of the relative genotype independence of this process. The results support the proposed idea of direct DNA uptake as an alternate and simple method for inserting foreign DNA into plant cells and its use for transformation of higher plants.

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“…Therefore, attention shifted to embryogenic suspension cells and embryogenic callus cultures derived from scutellar tissue of mature and immature embryos. A detailed and in depth account of the in vitro culture response of wheat from different explants is available in numerous reviews and so has not been dealt with in detail here (Bajaj, 1990;Maheshwari et al 1995;Vasil and Vasil, 1999). In recent years, with the development of suitable regeneration protocols, microspore embryos and immature inflorescences are emerging as suitable target tissues for genetic transformation experiments.…”

Section: Approaches For Wheat Transformationmentioning

confidence: 99%