In Vitro Culture to Eliminate Peanut Stripe Virus from Peanut Seed

Abstract

When excised seed axes of peanut seed, which tested serologicalpositive for peanut stripe virus (PStV),were grown in vitro, 37.5% of the developing plants were PStV-negative. When plants were regenerated from shoot-tips (1 em long) taken from in vitro-grown, virusinfected plants, 29.4% of them were virus-negative. PStV-positive plants obtained from virus-infected seed were grown for 12 wk on Murashige and Skoog (MS) medium supplemented with 40 mglL ribavirin (MSR) resulted in 78.6% which tested negative for PStV. All plants from seed which tested positive for PStV were 100%identified as negative if grown on MSR for 16 wk. Similarlyall plants were PStV negative when obtained from 1ernlong infected shoot tips (fromplants grown on MSor MSRmedium) ifsubsequently grown for 12wkon MSR medium.

Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro

“…Antivirals have been utilized for in vitro cultivation of plants, as a therapeutical supplement in integrated control programs targeted at plant viral diseases. Compounds such as Ribavirin (RBV) (1-β-D-ribofurasonyl-1,2,4 triazone-3-carboxamide) (Hansen & Lane, 1985;Griffiths et al, 1990;Chen & Sherwood, 1991;Toussaint et al, 1993;Prasada Rao et al, 1995), 5-Azacytidine (AZA) (4-amino-1-β-D-ribofuranosyl-5-triazin-2[1H]-one) and 3-Deazauridine (DZD) (4-hydroxi-1-β-Dribofuranosyl-2 [1H] pyridone) (Dunbar et al, 1993) have been successfully utilized for virus elimination in economically important crops, such as potato, peanut (Arachis hypogaea L.), apple (Pyrus malus L.) and Prunus spp.…”