In vitrocytotoxicity and catalytic evaluation of dioxidovanadium(v) complexes in an azohydrazone ligand environment

Abstract

Synthesis, characterization, in vitro cytotoxicity and catalytic potential of the dioxidovanadium(v) complexes of azohydrazones.

Study of DNA Interaction and Cytotoxicity Activity of Oxidovanadium(V) Complexes with ONO Donor Schiff Base Ligands

“…In continuation of our previous work on the synthesis, characterization, and biological studies of vanadium(V/IV) complexes [8,13,[31][32][33][34][35][36][37][38][39][40][41][42][43][44][45], here we report a new mononuclear dioxidovanadium(V) (1) as well as an oxido-bridged dinuclear oxidovanadium(V) (2) complex, each with a tridentate ONO donor Schiff base ligand derived from 2,4-dihydroxybenzaldehyde and 2-amino-4-nitrophenol. Considering the therapeutic potential of the synthesized polyphenolic ligand molecule [27,30,46], corresponding oxidovanadium(V) complexes were synthesized to further investigate their pharmacological activities such as DNA interaction and anticancer activities.…”

“…The spectrum of H 2 L exhibits two compounds connected via intermolecular hydrogen bonding as shown in Figure S2 due to which two equivalent sets of protons obtained in the NMR. The 1 H NMR spectra of H 2 L show singlet resonances in the downfield region in the range δ = 10.92-8.95, and 8.21 ppm due to -OH, and -CH (azomethine) protons, respectively [42]. The aromatic protons were observed in the expected range between δ = 8.20-6.27 ppm [37].…”

“…However, in 1, the spectra suggests a mononuclear vanadium(V) complex and it exhibits a singlet for each -OH and -HC=N in the region 10.34 and 9.39, ppm respectively [42]. The aromatic protons were observed in the expected range between δ = 8.59-6.77 ppm and additionally two sets of resonances that is, a quartet at δ = 3.08 and a triplet at 1.15 ppm were observed for N-CH 2 -and -CH 3 , respectively in the aliphatic region, which are attributed to the presence of a triethylammonium counterion [42]. In the case of 2 two equivalent sets of protons are observed which are attributed to dimerization of the complex through µ 2 -oxido-bridging.…”

“…Crystal data and refinement details are given in Table 5. The interaction of the ligand and its respective oxidovanadium(V) complexes (1 and 2) with CT-DNA was investigated by the absorption titration method with a Shimadzu spectrophotometer (UV-2450) [39,40,42,51]. The absorption titration of DNA was conducted by using a fixed concentration of metal complex (25 µM) in 50 mM Tris-HCl buffer (pH = 7.4), with gradual increases in concentration of the CT-DNA from 0 to 100 µM.…”

“…The cytotoxicity of the ligand molecule and its respective vanadium(V) complexes was evaluated against colon cancer (HT-29) and mouse embryonic fibroblast (NIH-3T3) cells using the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium) assay [42]. All cell lines were cultured in DMEM (Dulbecco s phosphate-buffered saline) medium supplemented with 10% FBS (Fetal bovine serum) and maintained at 37 • C in a CO 2 incubator (5% CO 2 ) and humidified atmosphere (95% humidity).…”