<i>In vitro</i>cytotoxicity assay of<i>Sauropus androgynus</i>on human mesenchymal stem cells

Yunita, Oeke; Yuwono, Mochammad; Rantam, Fedik Abdul

doi:10.1080/02772248.2013.798412

Cited by 9 publications

(5 citation statements)

References 13 publications

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“…These results are in good accordance with Morikawa et al [ 33 ] who reported no cytotoxic effect for hesperidin at 500 μM in adipocytes derived from human bone marrow stromal cells. Other authors have performed experiments treating hMSCs at different doses, not using isolated phenolic compounds but plant extracts containing kaempferol, hesperidin and apigenin among other phenolic compounds [ 34 , 35 ]. The only toxic effect was observed at the highest doses used (above 0.5 mg/mL).…”

Section: Discussionmentioning

confidence: 99%

Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes

et al. 2017

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BackgroundAdipocytes derived from human mesenchymal stem cells (MSCs) are widely used to investigate adipogenesis. Taking into account both the novelty of these MSCs and the scarcity of studies focused on the effects of phenolic compounds, the aim of the present study was to analyze the effect of apigenin, hesperidin and kaempferol on pre-adipocyte and mature adipocytes derived from this type of cells. In addition, the expression of genes involved in TG accumulation was also measured.MethodsPre-adipocytes were cultured from day 0 to day 8 and mature adipocytes for 48 h with the polyphenols at doses of 1, 10 and 25 µM.ResultsApigenin did not show an anti-adipogenic action. Pre-adipocytes treated with hesperidin and kaempferol showed reduced TG content at the three experimental doses. Apigenin did not modify the expression of the main adipogenic genes (c/ebpβ, c/ebpα, pparγ and srebp1c), hesperidin inhibited genes involved in the three phases of adipogenesis (c/ebpβ, srebp1c and perilipin) and kaempferol reduced c/ebpβ. In mature adipocytes, the three polyphenols reduced TG accumulation at the dose of 25 µM, but not at lower doses. All compounds increased mRNA levels of atgl. Apigenin and hesperidin decreased fasn expression. The present study shows the anti-adipogenic effect and delipidating effects of apigenin, hesperidin and kaempferol in human adipocytes derived from hMSCs. While hesperidin blocks all the stages of adipogenesis, kaempferol only inhibits the early stage. Regarding mature adipocytes, the three compounds reduce TG accumulation by activating, at least in part, lipolysis, and in the case of hesperidin and apigenin, also by reducing lipogenesis.ConclusionsThe present study shows for the first time the anti-adipogenic effect and delipidating effect of apigenin, hesperidin and kaempferol in human adipocytes derived from MSCs for the first time.

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Section: Discussionmentioning

confidence: 99%

Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes

et al. 2017

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“…11,12 The beneficial effects of antioxidative components of SA leaves have been proven in vitro and in vivo studies, although there is also reported some adverse health effects of intake the fresh SA leaves like bronchiolitis obliterans. [13][14][15][16] A study by Sai and Srividya (2002) 17 showed the hypoglycemic activity of aqueous SA leaf digest in human. Our previous study reported that antioxidant activity of chlorophyll extract from katuk leaves (CSA) showed the potential effect to cure sodium nitrate-induced anemia in female mice 18 and female Wistar rats 19 .…”

Section: Introductionmentioning

confidence: 99%

Hypoglycemic and Antianemia Effects of Chlorophyll from Sauropus androgynus (L) Merr Leaves in Rats

Suparmi¹,

Fasitasari²,

Martosupono³

et al. 2021

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Background: Beneficial effect of katuk (Sauropus androgynus L Merr) leaves (CSA) as one of medicinal plant in Indonesian jamu may potential to cure diabetes mellitus (DM) and iron deficiency anemia (IDA) as well as its well-known effect to increase breast milk production. Objectives: This study was aiming to explore the hypoglycemic effects of CSA in streptozotocininduced DM rats (STZ) and antianemia effects in IDA rats (FeD). Material and Methods: STZ male Wistar rats were treated with CSA, commercial chlorophyll (Chln) and glibencamide. Blood glucose levels and histological changes of liver, kidney and pancreas were analyzed after 14 days treatment. At separate experiment, FeD female Wistar rats were treated with CSA and Chln. The treatment was performed orally at the 1 st -20 th day of pregnancy. Levels of Hb, Fe and ferritin blood serum, number and body weight of the offspring were analyzed on 21 st day (post-partum). Results: Treatment of CSA for 14 days to diabetic animals demonstrated the hypoglycemic effect, however the histological analysis suggested that the scores of liver, kidney and pancreas were higher than that in the normal group. Moreover, CSA exerted a significant restorative effect by returning the levels of hemoglobin, serum iron, and serum ferritin. A fetus analysis suggested that the fetus body weight of in the CSA and Chln groups was similar to that in the normal group. Conclusion: CSA treatment are potential as a new herbal supplement to cure DM and IDA. However, the safety dose should be considered to prevent the toxicity.

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“…Toxicity tests were carried out to prevent adverse effects and to explain toxic effects and determine safety limits in relation to the use of a compound and the ability to inhibit protein denaturation (Madhu et al 2014). Yunita et al (2013) reported that SAL from different places has different levels of toxicity and this condition is presumably due to the secondary metabolism contents. To date, there are few studies that have investigated the phytochemical of SAL extract and this study is basic research to support the study of SAL extract supplementation in fish for the development of aquaculture by using natural materials.…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Phytochemical screening and toxicity of ethanol extract of Sauropus androgynus

et al. 2020

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Abstract. Awaludin, Kartina, Maulianawati D, Manalu W, Andriyanto, Septiana R, Arfandi A, Lalang Y. 2020. Short Communication: Phytochemical screening and toxicity of ethanol extract of Sauropus androgynus. Biodiversitas 21: 2966-2970. Katuk (Sauropus androgynus L.) (SAL) is a plant used to increase the production of breast milk for nursing mothers and lactating mammalian animals because this plant contains high sterols. Phytosterols are plant sterols known to play a role in the synthesis and secretion of progesterone and estradiol. Progesterone and estradiol are hormones that are involved in controlling the reproduction and mammary gland growth and development in mammalian animals. Estradiol also stimulates vitellogenin synthesis in oviparous animals such as avian and fish. The study was designed to determine the phytochemical contents and the level of toxicity of the SAL ethanol extract. The research was conducted in 4 phases i.e., (i) extraction of SAL samples using ethanol 70%, (ii) the analysis of phytochemical content of SAL extract using GCMS, (iii) toxicity test of SAL extract in giant tiger shrimp larvae, and (iv) data analysis. The results of GCMS analysis showed that the SAL extract was dominated by the fatty acid group (62.92%), phenol (15.3%), and terpenoids (4.03%). These compounds have roles in animal reproduction and production. The mortality data of giant tiger shrimp larvae were analyzed using probit analysis to determine the LC50 of ethanol extract of SAL. The results of toxicity test for 24 hours showed that the LC50 of the ethanol extract of SAL was 552.208 mg/L. The phytochemical analysis showed that SAL extract has dominant concentrations of fatty acid, phenol, and terpenoid that could be used to improve the reproduction and production of fish.

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In vitrocytotoxicity assay ofSauropus androgynuson human mesenchymal stem cells

Cited by 9 publications

References 13 publications

Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes

Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes

Hypoglycemic and Antianemia Effects of Chlorophyll from Sauropus androgynus (L) Merr Leaves in Rats

Short Communication: Phytochemical screening and toxicity of ethanol extract of Sauropus androgynus

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