<i>In Vitro</i>Cytotoxicity of a Series of Quassinoids from<i>Brucea javanica</i>Fruits Against KB Cells

Anderson, Margaret M.; O’Neill, Melanie J.; Phillipson, J. David; Warhurst, David C.

doi:10.1055/s-2006-960020

Cited by 67 publications

(43 citation statements)

References 4 publications

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“…In assessing selectivity of action against P. falciparum versus mammalian cells, we reported previously that the ratio of IC50s in vitro for chloroquine diphosphate is 347 (IC50 for KB cells/IC50 for P. falciparum KI) (1). In the present investigation, the least cytotoxic alkaloid, thalisopidine, had a cytotoxic activity/ antiplasmodial activity ratio of 455, which was more favorable than that of the more cytotoxic berbamine, which had a corresponding ratio of 39.5.…”

Section: Resultssupporting

confidence: 46%

“…Chloroquine diphosphate, emetine dihydrochloride, and podophyllotoxin were obtained from Sigma, and metronidazole was obtained from May & Baker. The methods used for the in vitro screening of the alkaloids for antiplasmodial, antiamoebic, and cytotoxic activities have been described in detail elsewhere (1,13,14,24) and will only be outlined here.…”

Section: Materuils and Methodsmentioning

confidence: 99%

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In vitro antiplasmodial, antiamoebic, and cytotoxic activities of a series of bisbenzylisoquinoline alkaloids

Marshall

Russell

Wright

et al. 1994

Antimicrob Agents Chemother

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Twenty-four bisbenzylisoquinoline alkaloids were screened for antiplasmoidal, antiamoebic, and cytotoxic activities by use of in vitro microtests. Eight of the alkaloids had antiplasmodial activity, with a 50% inhibitory concentration (IC50) of less than 1 microM against a multidrug-resistant strain of Plasmodium falciparum (chloroquine had an IC50 of 0.2 microM). The three alkaloids most active against Entamoeba histolytica, aromoline, isotrilobine, and insularine, had IC50s of 5 to 11.1 microM (metronidazole had an IC50 of 1.87 microM). None of the 24 bisbenzylisoquinoline alkaloids exhibited significant cytotoxicity against the KB cell line, the most toxic being berbamine, with an IC50 of 17.8 microM (the IC50 of podophyllotoxin was 0.008 microM). Bisbenzylisoquinoline alkaloids merit further investigation as potential novel antimalarial agents.

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Section: Resultssupporting

confidence: 46%

Section: Materuils and Methodsmentioning

confidence: 99%

In vitro antiplasmodial, antiamoebic, and cytotoxic activities of a series of bisbenzylisoquinoline alkaloids

Marshall

Russell

Wright

et al. 1994

Antimicrob Agents Chemother

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“…Further studies including curative dosage, effective route, and period of administration of bruceine A as well as a rational design of combination therapy are required. Bruceine A was found to have some toxic effects on treated animals [2,23]. The risk of side-effects is likely to increase with higher dosages of the drug; therefore, reduction of the risk needs to be considered.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Efficacy of Bruceine A, a Natural Quassinoid Compound Extracted from a Medicinal Plant, Bruced javanica, for Canine Babesiosis

et al. 2009

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ABSTRACT. Bruceine A, a natural quassinoid compound extracted from the dried fruits of Brucea javanica (L.) Merr., was evaluated for its antibabesial activity in vitro and in vivo. Bruceine A inhibited the in vitro growth of Babesia gibsoni in canine erythrocytes at lower concentration compared with the standard antibabesial drug diminazene aceturate and killed the parasites within 24 hr at a concentration of 25 nM. Oral administration of bruceine A at a dosage of 6.4 mg/kg/day for 5 days resulted in no clinical findings in a dog with normal ranges of hematological and biochemical values in the blood. Three dogs were infected with B. gibsoni and two of them were treated with bruceine A at a dosage of 6.4 mg/kg/day for 6 days from day 5 post-infection. An untreated dog developed typical acute babesiosis symptoms including severe anemia, high fever, and complete loss of appetite and movement. However, the two bruceine A-treated dogs maintained their healthy conditions throughout the experimental period of 4 weeks although complete elimination of parasites from the peripheral blood was not achieved and decreases in the packed cell volume and the erythrocyte and platelet counts were observed. Since natural quassinoid compounds have been used as traditional medicines for the treatment of various ailments including cancer and malaria, the present results suggest that bruceine A or other related compounds are potential candidates for the treatment of canine babesiosis.

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“…Crude extracts were evaluated against KB cells (human epidermoid carcinoma of the nasopharynx) according with the methodology described by Anderson et al (1991). Briefly, the cells were cultured in Eagles Minimum Essential Medium (MEM) with Earl's salts and 0.85% g/L of sodium bicarbonate, supplemented with non-essential amino acids (1%, v/v), 10% fetal bovine serum, L-glutamine (2 mM), penicillin (50 U/ mL) and streptomycin (50 μg/mL).…”

Section: Methodsmentioning

confidence: 99%

Activity against drug resistant‐tuberculosis strains of plants used in Mexican traditional medicine to treat tuberculosis and other respiratory diseases

Ramírez-Cabrera

González-Santiago

Garza‐González

et al. 2007

Phytotherapy Research

101

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Tuberculosis (TB) kills about 3 million people per year worldwide. Furthermore, TB is an infectious disease associated with HIV patients, and there is a rise in multidrug‐resistant TB (MDR‐TB) cases around the world. There is a need for new anti‐TB agents. The study evaluated the antimycobacterial activity of nine plants used in Mexican traditional medicine to treat tuberculosis and other respiratory diseases. Nasturtium officinale showed the best activity (MIC = 100 µg/mL) against the sensitive Mycobacterium tuberculosis. The following plants were active also but at 200 µg/mL: Citrus sinensis, Citrus aurantifolia, Foeniculum vulgare, Larrea tridentata, Musa acuminata and Olea europaea. Contrary to the above data, activity against drug‐resistant variants of M. tuberculosis was more evident, e.g. N. officinale was the most potent (MIC ≤ 100 µg/mL) against the four mono‐resistant variants tested; F. vulgare and O. europaea were active against all the resistant variants (MICs ≤ 100 µg/mL). The most susceptible variant was the isoniazid resistant, being inhibited by C. aurantifolia, C. sinensis and O. europaea (MIC = 25 µg/mL). These data point to the importance of biological testing of extracts against drug‐resistant M. tuberculosis isolates, and the bioguided assay of these extracts for the identification of lead compounds against MDR‐TB isolates. Copyright © 2007 John Wiley & Sons, Ltd.

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In VitroCytotoxicity of a Series of Quassinoids fromBrucea javanicaFruits Against KB Cells

Cited by 67 publications

References 4 publications

In vitro antiplasmodial, antiamoebic, and cytotoxic activities of a series of bisbenzylisoquinoline alkaloids

In vitro antiplasmodial, antiamoebic, and cytotoxic activities of a series of bisbenzylisoquinoline alkaloids

Evaluation of Efficacy of Bruceine A, a Natural Quassinoid Compound Extracted from a Medicinal Plant, Bruced javanica, for Canine Babesiosis

Activity against drug resistant‐tuberculosis strains of plants used in Mexican traditional medicine to treat tuberculosis and other respiratory diseases

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