<i>In vitro</i> degradation by mixed rumen bacteria of 17 mono‐ and sesquiterpenes typical of winter and spring diets of goats on Basilitica rangelands (southern Italy)

Malecky, Mostafa; Fedele, V.; Broudiscou, Laurent

doi:10.1002/jsfa.3486

Cited by 14 publications

(14 citation statements)

References 49 publications

(51 reference statements)

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“…This supports our hypothesis that the terpene input concentrations in this study were high enough to support maximum terpene disappearance rates, which are therefore acceptable estimates of potential degradation of terpenes in the rumen. In addition, ranking the 14 terpenes by their relative amounts recovered was highly consistent with the results of a trial conducted under both experimentally and analytically dissimilar conditions (Malecky et al, 2009), in that terpenes were incubated with mixed rumen bacteria at a concentration 167-fold lower than in this study, and they were, then, extracted from fermentation broths using a head-space solid phase micro-extraction procedure. The relative amounts recovery ranged from 0.3% for a-phellandrene to 93% for (Z)-linalool oxide and correlated well with the data of this study (Y) according to the following regression equation: Y 5 X 3 0.962 (60.084) 1 6.9 (64.0; s.d.…”

Section: Discussionsupporting

confidence: 85%

Degradation of terpenes and terpenoids from Mediterranean rangelands by mixed rumen bacteria in vitro

Malecky

Albarello

Broudiscou

2012

Animal

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This in vitro study aimed at estimating the disappearance rates of 14 terpenes and terpenoids after 24-h incubation with mixed bacteria from caprine rumens. These compounds comprised nine monoterpene hydrocarbons (d-3-carene, p-cymene, b-myrcene, (E)-and (Z)-b-ocimene, a-phellandrene, a-terpinene, g-terpinene and a-terpinolene), four oxygenated monoterpenes ((E)-and (Z)-linalool oxide, 4-terpinenol, a 1 g terpineol) and one sesquiterpene hydrocarbon (b-cedrene). They were individually exposed to goat rumen microflora for 24 h in 70 ml culture tubes at an input level of 0.5 ml/l. Terpenoids were the least degraded, 100% of (E)-linalool oxide, 95% of (Z)-linalool oxide, 91% of 4-terpinenol and 75% of terpineol remained intact after 24-h incubation. In contrast, a-terpinolene concentration in fermentation broth extracts was below quantification limit, thus indicating an extensive, if not complete, degradation by rumen bacteria. Only 2% of the initial amounts of a-phellandrene were recovered. The other monoterpenes and b-cedrene were partly degraded, with losses ranging from 67% for d-3-carene to 90% for (E)-b-ocimene. The corresponding rates of disappearance were between 2.67 and 4.08 mmol/ml inoculum per day.

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Section: Discussionsupporting

confidence: 85%

Degradation of terpenes and terpenoids from Mediterranean rangelands by mixed rumen bacteria in vitro

Malecky

Albarello

Broudiscou

2012

Animal

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“…However, herbivores rarely suffer ill effects, since they have evolved a range of physiological mechanisms for detoxifying PSCs (Hoffman 1989; Duncan and Popi 2008; Fink‐Gremmels, 2010). Biotransformation of terpenes in ruminants may occur both at the rumen (Hylemon and Harder, 1999; Malecky et al., 2009b) and the lower intestine, as well as through phase I and II enzymes (Torregrossa and Dearing, 2009). Clearance or detoxification by hepatic (Egorin et al., 1996) or urinary routes (McLean et al., 1993) may occur when the concentration in the organism rises above a threshold.…”

Section: Introductionmentioning

confidence: 99%

Terpenes transfer to milk and cheese after oral administration to sheep fed indoors

Poulopoulou

Zoidis

Massouras

et al. 2011

Animal Physiology Nutrition

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Terpenes have been proposed as potential biomarkers in verifying the diets of grazing animals. A study of the relationships between the intake of terpenes and their presence in animal tissues (blood and milk) as well as in the final product (cheese) was conducted. Eight dairy sheep were divided into two equal groups, representing control (C) and treatment group (T). In T group oral administration of a mixture of terpenes, α-pinene, limonene and β-caryophyllene, was applied over a period of 18 days. Blood and milk samples were collected regularly and terpenes were identified by extraction using petroleum ether and the solid phase micro-extraction (SPME) method, respectively, followed by GC-MS analysis. Cheese was produced, from C and T animals separately, twice during the period of terpenes oral administration. Terpenes contents and chemical properties of the produced cheeses were investigated. Limonene and α-pinene were found in all blood and milk samples of the T group after a lag-phase of 2 days, while β-caryophyllene was detected in few plasma samples and in all milk samples. None of the terpenes was traced in blood and milk of C animals. The contents of cheese, in dosed terpenes, presented a more complicated pattern suggesting terpenes non-credible as biomarkers. We conclude terpenes can be used as biomarkers for authentification of ewes' milk, but further research is required on factors affecting their transfer to dairy products from grazing diets.

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“…The effect of degradation in the rumen on terpenes would vary according to the nature of terpene. Malecky et al (2009) after using in vitro incubation of seventeen terpenes with mixed rumen bacteria from dairy goats obtained recovery rates different markedly among terpenes, partly in relation to the presence of oxygen and rings in the molecules. Mathison et al (1999) indicated that alfalfa (Medicago sativa) saponins were rapidly released into rumen fluid and extensively degraded in the digestive tract of sheep but no attempt was made to determine how closely the products of degradation were related to the original molecules.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of Degradation in Sacco in the Rumen on the Anthelmintic Properties of Parkia Biglobosa and Pterocarpus Erinaceus on Haemonchus Contortus

Dèdéhou¹,

Babatounde

Hounzangbe-Adote

2018

ESJ

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Parkia biglobosa and Pterocarpus erinaceus are traditionally used to treat various ailments including helminth infections. This study was undertaken to evaluate the effect of the rumen degradability on anthelmintic properties of the two plants and to examine the possible role of tannins and/or polyphenols on these properties. Hydro-acetone extracts of in sacco degradability residues of P. biglobosa pods and P. erinaceus leaves were screened in vitro to determine the possible anti-parasitic effects against eggs and infective larvae of Haemonchus contortus. In addition, the possible involvement of tannins and/or polyphenols was examined by comparing the levels of inhibition of larval migration obtained with the same extracts, after of not addition of PVPP. Extracts of both plants induced significant egg hatch inhibition (p < 0.001). The effects were dose and incubation period dependent. Extracts of both plants have shown again remarkable larval migration inhibition compared to the PBS (p < 0.05). The effect of incubation period was not significant (p > 0.05). These results suggest that the plants did not lose their anthelmintic properties after rumen degradation. The use of the PVPP indicated for almost all of the extracts that tannins and/or polyphenols are largely involved in the effect. Complementary investigations are necessary to understand the metabolism of these plants in the digestive tract of the animals.

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In vitro degradation by mixed rumen bacteria of 17 mono‐ and sesquiterpenes typical of winter and spring diets of goats on Basilitica rangelands (southern Italy)

Cited by 14 publications

References 49 publications

Degradation of terpenes and terpenoids from Mediterranean rangelands by mixed rumen bacteria in vitro

Degradation of terpenes and terpenoids from Mediterranean rangelands by mixed rumen bacteria in vitro

Terpenes transfer to milk and cheese after oral administration to sheep fed indoors

In Vitro Effect of Degradation in Sacco in the Rumen on the Anthelmintic Properties of Parkia Biglobosa and Pterocarpus Erinaceus on Haemonchus Contortus

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