No abstract

The need for new anthelmintic with no chemical residues is becoming urgent. In a program aiming at the evaluation of plant as sources of new active molecules, the anthelmintic activities of the essential oils (EOs) obtained from either Zanthoxylum zanthoxyloides seeds or Newbouldia laevis leaves were evaluated against Strongyloides ratti by analyzing the results of two in vitro bioassays. These two plants and their tested parts were retained after an ethnopharmacology survey that confirmed their use by small-scale farmers for treatment of small ruminants affected by digestive helminths. The plants were harvested in Benin, and their EO were obtained by hydrodistillation. The EO yield of extraction was 0.65% (w/w) of for Z. zanthoxyloides seeds and 0.05% (w/w) for N. laevis. The chemical compositions of the two EOs were analyzed by gas chromatography coupled with mass spectrometry. The major constituents of the EO from Z. zanthoxyloides consisted of the following compounds: γ-terpinene (18 %), undecane (15 %), valencene (8.3 %), decanal (8.3 %), and 3-carene (6.7 %). In contrast, the major constituents of the EO from N. laevis leaves consisted of the following compounds: β-caryophyllene (36 %) and eugenol (5.8 %). An egg-hatching inhibition (EHI) assay was developed and a larval migration inhibition assay was used on S. ratti to examine the effects of the EOs and to evidence their inhibitory concentrations (IC(50) and IC(90)) values on this nematode. Furthermore, the toxicity of the two EOs on Vero cell line was evaluated. When tested on S. ratti egg hatching, the two EOs resulted in similar IC(50) values (19.5 and 18.2 μg/ml for Z. zanthoxyloides and N. laevis, respectively), which were about sevenfold higher than that of the control (thiabendazole, IC(50) = 2.5 μg/ml). Larval migration was inhibited at similar concentrations for: Z. zanthoxyloides (IC(50) = 46 μg/ml), N. laevis (IC(50) = 51 μg/ml), and the control [levamisole (IC(50) = 36 μg/ml)]. No cytotoxicity was found on Vero cells because both EOs had IC(50) values higher than 50 μg/ml. Therefore, we have concluded that the EOs from two plants, used in folk medicine, may contain compounds with anthelmintic activity and could be used as improved traditional medicines or, at least, as food additives in a combined treatment for the control of helminth infections.

L’efficacité antiparasitaire des graines de papaye (Carica papaya) a été testée sur les strongles gastro-intestinaux. L’étude a porté sur 80 moutons répartis en cinq lots de 16 animaux (dix brebis et six agneaux) : un lot témoin, un lot témoin traité avec de l’albendazole à la dose de 5 mg/kg et trois lots ayant reçu de la poudre de graines de papaye séchées au soleil ou à l’étuve à des doses de 100, 200 ou 400 mg/kg de poids vif. Les taux d’infestation par les strongles gastrointestinaux des ovins ont varié en fonction du temps d’un lot à l’autre. L’efficacité des graines de papaye a été optimale à la dose de 200 mg/kg de poids vif avec un taux d’efficacité supérieur à 80 p. 100 sur les strongles, dix jours après le traitement. L’hématocrite est resté stable chez tous les animaux traités à l’albendazole ou aux graines de papaye. Il semble qu’un traitement avec des graines séchées à l’étuve, traitement plus efficace que lorsque les graines sont séchées au soleil, protège les ovins des effets anémiants des nématodes.

Twenty four (24) dairy ewes at the 4th stage of lactation were used to assess the milk production and serum prolactin level of ewes treated with leaf’s powder of Spondias mombin. The lactating ewes were selected and divided into 4 groups of 6 ewes per group: control group (no fed powder of Spondias mombin), Galactin group (no fed powder of Spondias mombin but treated with lactogenic drug) and groups treated with powder of Spondias mombin (group single dose and group double dose). The parameters measured include daily milk production, body weight of lambs and ewes. The resulted outcomes indicate that ewes that received powder of S. mombin and Galactin produced more milk than controls. The average daily increases in milk production were 9.92%, 14.25% and 18.88% in single dose, double dose and Galactin groups, respectively compared to control group. Lamb weight gain was also significantly higher than that in the control group. The treatments did not influence body weight of ewe. The daily weight gain of 93.12; 97.47; 99.07; 86.76 g/day were found in single dose, double dose, Galactin and control groups, respectively. The results of the blood analysis of hormone showed that serum prolactin levels increased in the group treated with powder and Galactin compared to control group.

Effet des feuilles de Moringa oleifera et de Commelina benghalensis sur les performances de croissance et les caractéristiques de carcasse des cochons d'Inde au Sud-Bénin