In vitro effects of four tropical plants on three life-cycle stages of the parasitic nematode, Haemonchus contortus

Hounzangbe-Adote, M.S.; Paolini, V.; Fourasté, I.; Moutaïrou, Kabirou; Hoste, Hervé

doi:10.1016/j.rvsc.2004.05.009

Cited by 159 publications

(94 citation statements)

References 24 publications

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“…Though, the efficacy was not comparable with the levamisole, the reference drug used for the control of the haemonchosis of sheep and goat in field conditions but research out is of considerable importance. The present finding is in agreement with the previous published research work (Hounzangbe-Adote et al 2005). Seed extract also contains high concentration of total phenolic flavonoid and tannin contents.…”

Section: Resultssupporting

confidence: 94%

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Anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against Haemonchus contortus of small ruminants

Singh¹,

Singh²,

Verma³

et al. 2014

J Parasit Dis

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Haemonchus contortus infection is the major hurdle in growth and productivity in small ruminants especially in tropical countries. Indiscriminate and long term use of currently available chemotherapeutic agents lead to development of resistance in microorganisms and residual problems in population. Therefore, present study was undertaken to evaluate the anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against adult H. contortus worms of small ruminants. Proximate analysis of seed powder shows high crude protein, Cu, Zn and Ca concentration. Phytochemical ingredients of extract revealed high total phenolic, flavonoids and tannin contents. In vitro experimental trial revealed complete mortality of H. contortus worms at the concentration of 100 mg/ml at the time exposure of 8 h. At 50 mg/ml concentration the mortality at 6 and 8 h were 45.45 ± 4.55 and 63.64 ± 4.54 %. Median lethal concentration (LC 50 ) at 6 and 8 h were 59.90 and 28.92 mg/ ml, respectively. Levamisole at concentration of 0.5 mg/ml caused 50 % mortality at 2 h post exposure and 100 % mortality at 4 h post exposure. The results confirm the cidal effect of the aqueous extract of Z. armatum DC. seeds on adult H. contortus worms.

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Section: Resultssupporting

confidence: 94%

“…have promising anthelmintic property especially for roundworms (Mehta et al 1981, Siddiqui and Garg 1990, Hounzangbe-Adote et al 2005. The Z. armatum DC., a shrub to small tree of family Rutaceae is widely distributed in India from Jammu & Kashmir to North East states.…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against Haemonchus contortus of small ruminants

Singh¹,

Singh²,

Verma³

et al. 2014

J Parasit Dis

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“…The discrepancy between the results of anthelmintic efficacy in vitro and in vivo has been attributed to several factors, including availability and/or concentration of active compounds in the preparation of plant material (HOUNZANGBE-ADOTE et al, 2005) and bioavailability and biotransformation of these compounds in animal body (VANDAMME; ELLIS, 2004). In polygastric animals, ruminal microorganisms can affect active chemical constituents of plants and reduce their bioavailability, and this may have contributed to low anthelmintic efficacy of A. sisalana juice found in the present study.…”

Section: Introductioncontrasting

confidence: 60%

Evaluation of anthelmintic activity of liquid waste of Agave sisalana (sisal) in goats

Domingues

Botura

Cruz

et al. 2010

Rev. Bras. Parasitol. Vet.

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It was evaluated the anthelmintic activity of Agave sisalana (sisal) juice against gastrointestinal nematodes and its potential toxic effects in goats. In vitro tests showed more than 95% reduction in larval counts of the genus Haemonchus spp. at concentrations between 86.5 and 146.3 mg.mL -1. In vivo the percent reduction of larvae of the fourth (L4) and fifth (L5) stages of Haemonchus, Oesophagostomum and Trichostrongylus was less than 95% in groups GI and GII, and between 80 and 90% in group GIII. A. sisalana juice at the concentrations tested in vitro was effective against gastrointestinal nematodes in goats; however, its anthelmintic efficacy was reduced when administered to animals.Keywords: Agave sisalana, anthelmintic, goats, nematodes, sisal. ResumoFoi avaliada a atividade anti-helmíntica do suco de Agave sisalana (sisal) contra nematódeos gastrintestinais e possíveis efeitos tóxicos em caprinos. Nos testes in vitro, encontrou-se redução superior a 95% na contagem de larvas do gênero Haemonchus spp. nas concentrações entre 86,5 e 146,3 mg.mL -1 . In vivo, o percentual de redução de larvas de quarto (L4) e quinto (L5) estágios de Haemonchus, Oesophagostomum e Trichostrongylus foi inferior a 95% para o GI e GII, e entre 80 e 90% para o GIII. O suco de A. sisalana nas concentrações testadas in vitro foi efetivo contra nematódeos gastrintestinais de caprinos, apresentando, no entanto, reduzida eficácia anti-helmíntica quando administrado nos animais.Palavras-chave: Agave sisalana, anti-helmíntico, caprinos, nematódeos, sisal.The goat industry is a livestock activity of great socioeconomic importance in Northeastern Brazil, but its operation has been limited by frequent gastrointestinal nematode infections (GNI) and inappropriate drug use, which has led to resistance of these parasites to common anthelmintics.Given the interest in the use of waste liquid of A. sisalana and lack of scientific evidence regarding its potential anthelmintic activity, the objective of the present study was to evaluate the effect of A. sisalana juice against gastrointestinal nematodes and its potential toxic effects in goats.Agave sisalana plants were collected in Valente, Bahia. They were identified by botanists and voucher specimens (No. 838) were deposited in the herbarium of the Universidade Estadual de Feira de Santana.A. sisalana pulp and juice were collected through mechanical defibration of the leaves. The juice was obtained after filtration three times in filter paper and stored at 4 °C for use in in vivo tests. For in vitro tests, the juice was dried and frozen. Five concentrations were used in this study : 146.3, 112.5, 86.5, 66.5, 51.1 and 39.3 mg.mL -1 , which were tested in triplicate. To validate the results, the same experiment was repeated three times.Larval cultures (UENO; GONÇALVES, 1998) consisted of 2 g of feces, 2 g of sawdust, and 2 mL of juice. Positive and negative controls were treated with doramectin (0.0625 mg.mL -1 ) and distilled water, respectively.Twenty-four racially undefined goats b...

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“…This test was performed according to Hounzangbe-Adote et al (2005). Adult worms were collected from an experimentally infected lamb six weeks after infection.…”

Section: In Vitro Anthelmintic Assaysmentioning

confidence: 99%

Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity

Akkari¹,

B’chir²,

Hajaji³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:Capparis spinosa is cultivated for several proprieties and the different parts of the plant (flower buds, fruits, leaf and seeds) have been employed in drugs, foods and cosmetics. This study aimed to explore the antioxidant potential effect and to assess the anthelmintic efficacy of an aqueous extract of Capparis spinosa. In vitro antioxidant activity of aqueous extracts from the fresh leaves and flower buds of C. spinosa was measured by determining free radical-scavenging activity against DPPH and ABTS radical cations. In vitro anthelmintic activities against eggs and adult worms of Haemonchus contortus from sheep were investigated in comparison to albendazole. DPPH and ABTS activities of both aqueous extracts increased in a dose dependent manner. The IC 50 values of aqueous extracts from fresh leaves, flowers buds and ascorbic acid in the DPPH radical scavenging assay were 101.40; 70.40 and 57.56 μg/ml, respectively. The IC 50 values of aqueous extracts fresh leaves, flowers buds and ascorbic acid in the ABTS radical scavenging assay were 110; 87.89 and 58.17 μg/ml, respectively. Both plant extracts showed ovicidal activity at all tested concentrations. The aqueous extract from flower buds (IC 50 = 2.76 mg/ml) showed higher inhibitory effects (P < 0.05) than aqueous extract from leaves (IC 50 = 8.54 mg/ml) in the egg hatching assay. The aqueous extract from flower buds inhibited more worms than the aqueous extract from leaves at all tested concentrations. After 6 h of exposure, the highest tested concentrations of aqueous extracts from flower buds and leaves induced 100 and 41.66% inhibition of motility, respectively. These results show for the first time that C. spinosa possesses in vitro anthelmintic properties which may be related to its high content of phenolic compounds such as flavonoids and tannins. Keywords: Capparis spinosa; free radical-scavenging ability; anthelmintic; Haemonchus contortusList of abbreviations ABTS = 2,2'-azinobis-[3-ethylbenzthiazoline-6-sulfonic acid]; DMSO = dimethyl sulfoxide; DPPH = 2,2-diphenyl-1-picrylhydrazyl; HPLC/MS = chromatography/mass spectrometry; PBS = phosphate-buffered saline Parasite infestations of livestock species are especially prevalent in developing countries due to poor management practices and inadequate control measures (Dhar et al. 1982). The main control measures for parasitic diseases largely rely on the repeated use of anthelmintics. However, resistance to various groups of anthelmintics is constantly increasing in worm populations, especially those found in small ruminants (Waller 1997;Van Wyk et al. 1999). In addition, the increasing pressure from consumers to limit the use of chemical substances in livestock industry merits consideration. These two issues underpin the current search for alternatives or rather complementary solutions to chemotherapy to control gastrointestinal parasitism in sheep and goats. There is an increasing body of evidence that stockowners rely on ethno-veterinary medicine as an alternative and as a sustain-

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In vitro effects of four tropical plants on three life-cycle stages of the parasitic nematode, Haemonchus contortus

Cited by 159 publications

References 24 publications

Anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against Haemonchus contortus of small ruminants

Anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against Haemonchus contortus of small ruminants

Evaluation of anthelmintic activity of liquid waste of Agave sisalana (sisal) in goats

Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity

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