In vitroeffects of berberine sulphate on the growth and structure ofEntamoeba histolytica,Giardia lambliaandTrichomonas vaginalis

Kaneda, Yoshimasa; Torii, Motomi; Tanaka, Tomoo; Aikawa, Masamichi

doi:10.1080/00034983.1991.11812586

Cited by 89 publications

(47 citation statements)

References 22 publications

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“…Berberine, an isoquinoline quarternary ammonium alkaloid isolated from the plants genera Berberis and Coptis, has anti-microbial, anti-diarrheic, and anti-inflammatory properties (Ckless et al, 1995;Kaneda et al, 1991;Taylor et al, 1999). Despite extensive literatures on the pharmacology, little is known about the pharmacokinetics and transport characteristics of the drug, except that the oral bioavailability in the beagle dog is low (Sheng et al, 1993) and it is a substrate of P-glycoprotein (P-gp), multidrug resistance associated protein (MRP1) (Maeng et al, 2002;Shitan et al, 2007), and organic cation transporters (OCTs) (Tsai and Tsai, 2004).…”

Section: Introductionmentioning

confidence: 99%

Effect of ion-pair formation with bile salts on the in vitro cellular transport of berberine

et al. 2008

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The objective of this study was to examine the effect of ion-pair complexation with endogenous bile salts on the transport of a quarternary ammonium organic cationic (OC) drug, berberine, across the Caco-2 and LLC-PK1 cell monolayers. The basolateral-to-apical (BL-AP) transport of berberine in Caco-2 cells was temperature dependent and 10-fold higher than that of the apical-to-basolateral (AP-BL) transport. Similar results were observed for the transport of berberine across the LLC-PK1 cells. Moreover, the BL-AP transport in the Caco-2 cells was significantly reduced by the cis-presence of P-glycoprotein (P-gp) inhibitors such as cyclosporine A, verapamil, and digoxin. These results suggest that an efflux transporter, probably P-gp, is involved in the Caco-2 cell transport. The K m and V max values for the carrier-mediated transport were estimated to be 83.4 mM and 7640 pmole/h/cm 2 , respectively. The apparent partition coefficient (APC) of berberine between n-octanol and a phosphate buffer (pH 7.4) was increased by the presence of an organic anion (OA), taurodeoxycholate (TDC, a bile salt), suggesting the formation of a lipophilic ion-pair complex between an OC (berberine) and an OA (TDC). Despite the ion-pair complexation, however, the BL-AP transport of berberine across the Caco-2 and LLC-PK1 cells was not altered by the cis-presence of bile salts or the rat bile juice. This is consistent with the reportedly unaltered secretory transport of a quarternary ammonium compound, tributylmethylammonium (TBuMA), across the Caco-2 cell monolayers in the cis-presence of bile salts or the rat bile juice, but not with our previous report in which the secretory transport of TBuMA across the LLC-PK1 cell was increased in the cis-presence of TDC. Therefore, the effect of ion-pair formation with the bile components or bile salts on the secretory transport of OCs appears to depend on the molecular properties of OCs (e.g., molecular weight, lipophilicity and affinity to relevant transporters) and the characteristics of cell strains (e.g., expression and contribution of responsible transporters to the transport).

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Section: Introductionmentioning

confidence: 99%

Effect of ion-pair formation with bile salts on the in vitro cellular transport of berberine

et al. 2008

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“…Berberine as an herbal medicine has been used to treat bacteria-associated diarrhea, intestinal parasitic infections, and ocular trachoma infections for several decades. Several mechanisms attribute to its efficacy, including decreasing enterotoxin-induced intestinal secretion of water and electrolytes (33), bactericidal activity (2), and inhibition of protozoan growth (17).…”

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confidence: 99%

Berberine promotes recovery of colitis and inhibits inflammatory responses in colonic macrophages and epithelial cells in DSS-treated mice

Yan

Wang

Shi

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

164

126

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Inflammatory bowel disease (IBD) results from dysregulation of intestinal mucosal immune responses to microflora in genetically susceptible hosts. A major challenge for IBD research is to develop new strategies for treating this disease. Berberine, an alkaloid derived from plants, is an alternative medicine for treating bacterial diarrhea and intestinal parasite infections. Recent studies suggest that berberine exerts several other beneficial effects, including inducing anti-inflammatory responses. This study determined the effect of berberine on treating dextran sulfate sodium (DSS)-induced intestinal injury and colitis in mice. Berberine was administered through gavage to mice with established DSS-induced intestinal injury and colitis. Clinical parameters, intestinal integrity, proinflammatory cytokine production, and signaling pathways in colonic macrophages and epithelial cells were determined. Berberine ameliorated DSS-induced body weight loss, myeloperoxidase activity, shortening of the colon, injury, and inflammation scores. DSS-upregulated proinflammatory cytokine levels in the colon, including TNF, IFN-γ, KC, and IL-17 were reduced by berberine. Berberine decreased DSS-induced disruption of barrier function and apoptosis in the colon epithelium. Furthermore, berberine inhibited proinflammatory cytokine production in colonic macrophages and epithelial cells in DSS-treated mice and promoted apoptosis of colonic macrophages. Activation of signaling pathways involved in stimulation of proinflammatory cytokine production, including MAPK and NF-κB, in colonic macrophages and epithelial cells from DSS-treated mice was decreased by berberine. In summary, berberine promotes recovery of DSS-induced colitis and exerts inhibitory effects on proinflammatory responses in colonic macrophages and epithelial cells. Thus berberine may represent a new therapeutic approach for treating gastrointestinal inflammatory disorders.

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“…Berberine extracts and salts have demonstrated growth inhibition of Entamoeba histolytica, Trichomonas vaginalis 20 , Leishmania donovani 21 and Giardia lamblia, 23 with crude extracts being more effective than berberine salts. 22 Clinical trials conducted in India showed berberine administration improved gastrointestinal symptoms and resulted in a marked reduction in Giardia-positive stools.…”

Section: Anti-parasitic Effectsmentioning

confidence: 99%

Therapeutic Aspects of Goldenseal

Asmi¹,

Lakshmi²

2013

Int. Res. J. Pharm.

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Goldenseal, commonly known as yellow puccoon has wide therapeutic activity. The bioactive components present are isoquinoline alkaloids, hydrastine, berberine and canadine. Berberine is used to treat heart failure also have sedative actions, anti secretory, anti parasitic, anticancerous actions. Hence an attempt was taken to review the pharmacological actions of Goldenseal with respect to its role in dental practice.

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In vitroeffects of berberine sulphate on the growth and structure ofEntamoeba histolytica,Giardia lambliaandTrichomonas vaginalis

Cited by 89 publications

References 22 publications

Effect of ion-pair formation with bile salts on the in vitro cellular transport of berberine

Effect of ion-pair formation with bile salts on the in vitro cellular transport of berberine

Berberine promotes recovery of colitis and inhibits inflammatory responses in colonic macrophages and epithelial cells in DSS-treated mice

Therapeutic Aspects of Goldenseal

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