<i>In vitro</i>effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells

Chen, Jiayong; Liang, Daoming; Gan, Ping; Zhang, Yi; Lin, Jie

doi:10.3748/wjg.v10.i8.1132

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…Then, after we detected volume of tumor, tumor growth inhibitory rate and cell cycle, we thought that the short term usage of rhGH would not accelerate the growth of human gastric cancer cells and it was in accord with our earlier studies and other data [14] [15] [17]. The results lay the foundation for next study.…”

Section: Discussionsupporting

confidence: 75%

“…In the mean IGF-1 IGF1R IGFBP3 GAPDH 1 2 3 4 time, the short term treatment of which rhGH had decreased the rate of IGF-I/IGFBP-3, especially when the treatment was associated with chemical therapy. In our earlier and before researches, it showed that the short term usage of rhGH would not accelerate the growth of human gastric cancer cells [13] [14]. Considering the results of both research, it should be suggested that the short term usage of rhGH would decrease the rate of IGF-I/IGFBP-3 in blood and would increase the protein level of IGFBP-3 in blood and gastric cancer tissue both, and the increased IGFBP-3 would bind with IGF-I as well as competitively inhibited the combination between IGF-I and its receptors, so it was one of mechanism of that the short term usage of rhGH would not accelerate the growth of human gastric cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Lots of overseas clinical data showed that hGH did not improve cancer growth and could decrease peroperative complications incidence and death rate in postoperative patients with gastrointestinal malignant tumor [9]- [13]. Our previous empirical study has also demonstrated that rhGH did not accelerate the proliferation of human gastric cancer cell line BGC823 in vitro and in vivo [14] [15]. But mechanism that rhGH did not accelerate the proliferation of human gastric cancer cell was unknown.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Exogenous Growth Hormone on Growth Hormone-Insulin-Like Growth Factor Axis of Human Gastric Cancer Cell

Liang¹,

Zhang²,

Chen³

et al. 2014

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Aim: To study effects of recombinant human growth hormone (rhGH) on growth hormone-insulin-like growth factor axis (GH-IGFs) of human gastric cancer cell in vivo in order to reveal part mechanism of growth effects of rhGH on gastric cancer. Methods: Nude mice were randomly divided into control group, cisplatin (DDP) group, rhGH group and DDP + rhGH group after human gastric cancer xenograft model of node mice was successfully founded and drugs were used for 6 days. We investigated volume of tumor, inhibitory rate of tumor and cell cycle by slide gauge and flow cytometry. In addition, We also respectively investigated insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) of blood serum of nude mice, IGF-ImRNA, insulin-like growth factor-I receptor (IGF-IR) mRNA and IGFBP-3 mRNA of xenograft of nude mice by enzyme linked immunosorbent assay (ELISA) and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) on the first day of completing use of drugs later. Results: Tumor grew obviously slowly and tumor inhibitory rate obviously rose in DDP group and DDP + rhGH group compared with control group and rhGH group (p < 0.05), but they were not remarkably different between DDP group and DDP + rhGH group or between control group and rhGH group. Cells of gastric cancer xenograft in S phase distinctly diminished in DDP group and DDP + rhGH group compared with control group and rhGH group (p < 0.05), but they were not statistically significant between DDP group and DDP + rhGH group or between control group and rhGH group. IGF-I and IGFBP-3 of blood serum of nude mice obviously rose, but ratio of IGF-I and IGFBP-3 obviously lowered in rhGH group and DDP + rhGH group compared with control group and DDP group (p < 0.05). Expressions of IGF-I mRNA and IGF-IR mRNA were not obviously different in all groups. But expression of IGFBP-3 mRNA obviously increased in rhGH group, DDP group and DDP + rhGH group compared with control group; meanwhile, expression of IGFBP-3 mRNA also obviously increased in DDP + rhGH group compared with control group, DDP group and rhGH group. Conclu-* Corresponding author. D. M. Liang et al. 260sion: Our results indicated rhGH in short-time use did not improve proliferation of human gastric cancer cells and its mechanism was possible that rhGH in short-time use raised simultaneously IGF-I and IGFBP-3 of blood serum and increased IGFBP-3 mRNA, but degraded ratio of IGF-I and IGFBP-3 of blood serum in human gastric cancer cells.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Exogenous Growth Hormone on Growth Hormone-Insulin-Like Growth Factor Axis of Human Gastric Cancer Cell

Liang¹,

Zhang²,

Chen³

et al. 2014

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“…Swerdlow et al [28] suggested that hGH did not increase the risk of recurrence of childhood brain tumours. Our previous studies have demonstrated that rhGH does not increase gastric cancer growth, which makes rhGH safe for use with anticancer drugs in vitro and in vivo [29,30] .…”

Section: Discussionmentioning

confidence: 99%

Effects of Human Growth Hormone on Haematopoietic Recovery of Rats Receiving Chemotherapy

et al. 2008

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Human growth hormone (hGH) is a pleiotropic cytokine targeting a variety of tissues. Its protective effects on haematopoiesis during treatment with chemotherapeutic drugs were investigated. Sprague-Dawley rats were intraperitoneally administered with both carboplatin and 5-fluorouracil together with or without recombinant hGH (rhGH) at a dose of 1 IU/kg/day. Body weight, full blood count, bone marrow differential count and expression of proliferating cell nuclear antigen (PCNA) in bone marrow were measured weekly for 4 weeks in chemotherapy-treated (CT) or chemotherapy plus rhGH-treated (CT+GH) animals. During the first week, body weight, white blood cell count, haematopoietic count and reticulocyte count were decreased in the CT group as well as in the CT+GH group, but to a lesser extent in the latter group (CT vs. CT+GH group, p < 0.05). Further decreases were also prevented in the CT+GH group. Myeloid cells were extremely hypoplastic in the CT group, while in the CT+GH group, myeloid cells recovered from obviously hypoplastic to excessively hyperplastic in the first 2 weeks, and the myeloid karyocyte count increased significantly (CT+GH vs. CT group, p < 0.05). PCNA-positive cell count in the CT+GH group was also significantly higher than in the CT group (CT+GH vs. CT group, p < 0.05). Thus, rhGH showed a promising protective effect on body weight loss and haematopoietic recovery of chemotherapy-treated rats, which may be useful in clinic to reduce the side effects of chemotherapy.

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“…Гормон росту сприяє синтезу білка, мобілізації ліпідного обміну та регулює баланс азоту у клітині [8]. Додатково, за даними Costoya J.A.…”

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Вплив гормону росту (rhGH) та Biolaminin 521 LN на проліферативну активність стовбурових клітин кота

Мазуркевич¹,

Ковпак²,

Ковпак³

2018

visnyk

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Досліджено вплив гормону росту (rhGH) у різних концентраціях та Biolaminin 521 LN на проліферативну активність та генетичну стабільність стовбурових клітин, отриманих з кісткового мозку, жирової тканини та міокарду кота. Встановлено, що гормон росту у низьких концентраціях (10 нг/мл) позитивно впливає на проліферативну активність стовбурових клітин, отриманих із жирової тканини та міокарду кота. Разом із тим, на культуру стовбурових клітин кісткового мозку ефект низьких концентрацій rhGH був протилежний. Культивування стовбурових клітин за додавання Biolaminin 521 LN призвело до достовірного збільшення індексу проліферації у всіх досліджуваних культурах. За даними цитогенетичного аналізу встановлено, що додавання гормону росту у культуральне середовище не призводить до достовірного збільшення кількості генетичних помилок. Водночас, додавання Biolaminin 521 LN призводить до зменшення кількості клітин із зміненим каріотипом (у порівнянні з контролем) у всіх досліджуваних культурах. The usage of cell technology in clinical practice requires a large amount of cell material. It in turn provokes the development of methods that will allow to obtain a greater amount of cell material for a much shorter period of time. According to literary sources it is known that the growth hormone and the Biolaminin LN 521 can have a positive influence on the mitotic activity of stem cells. Considering the differences in the cell composition of the cell cultures obtained from different tissues, the effects of recombinant human growth hormone (rhGH) and Biolaminin 521 LN will be different. Therefore, our aim was to study the effects of recombinant human growth hormone (rhGH) in various concentrations and to study the Biolaminin LN 521 for the proliferative activity of stem cells obtained from bone marrow, adipose tissue and cardiac muscle of cat. The effects of recombinant human growth hormone (rhGH) in various concentrations and the effects of Biolaminin LN 521 for proliferative activity and genetic stability of stem cells obtained from bone marrow, adipose tissue and cardiac muscle of cat were studied. It was found that the growth hormone has a positive effect on the proliferative activity of stem cells in cell cultures of adipose tissue and cardiac muscle of cat at low concentrations (10 ng/ml), while the effect on the cell culture of bone marrow stem cells was the opposite. According to the cytogenetic analysis it was found that adding recombinant human growth hormone to the culture media does not lead to a significant increase in the number of genetic errors, while adding the Biolaminin 521 LN leads to a decrease in the number of cells with altered karyotype (in comparison with control) in all the studied cell cultures.

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In vitroeffects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells

Cited by 13 publications

References 27 publications

Effects of Exogenous Growth Hormone on Growth Hormone-Insulin-Like Growth Factor Axis of Human Gastric Cancer Cell

Effects of Exogenous Growth Hormone on Growth Hormone-Insulin-Like Growth Factor Axis of Human Gastric Cancer Cell

Effects of Human Growth Hormone on Haematopoietic Recovery of Rats Receiving Chemotherapy

Вплив гормону росту (rhGH) та Biolaminin 521 LN на проліферативну активність стовбурових клітин кота

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