In vitroevaluation ofEclipta albaagainst serogroups ofLeptospira interrogans

Abstract

Leptospirosis is now acknowledged as the most widespread zoonoses in the world. Hundreds of cases occur in India every year accounting for considerable morbidity and sizable mortality. Several studies have delineated the epidemiology, pathology and variable clinical features of this condition. The present study comprises the importance and utilization of traditional based medicines to overcome the adverse reaction by conventional drugs and standardize the technology. The antileptospiral activity of Eclipta alba L. was well studied by both tube dilution and micro dilution techniques and the result showed better inhibitory action against various serogroups of Leptospira interrogans. L. australis, L. autumnalis and L. grippotyphosa are inhibited by both water and ethanol extract by tube dilution technique. The MIC level observed are 50 μg and 100 μg respectively. Similarly acetone extract, Icterohaemorrhagiae was responded to 200 μg/ml as MIC whereas in petroleum ether extract, no inhibition was observed. In the case of micro dilution technique, the entire inhibition rates are supported to the tube dilution technique. It showed that the micro dilution technique is the best method where we obtained the results within 30 minutes; at the same time tube dilution technique takes minimum of 7 days to provide the result.

Optimization of Ultrasonic‐Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology

“…The medicinal plant Eclipta prostrasta L., an herb belonging to the family Asteraceae, is widely distributed in tropical and subtropical regions under conditions where plants can be water logged (Christybapita and others 2007; Prabhu and others 2008). This plant has been reported to possess several medicinal properties.…”

Antileptospiral, Antioxidant and DNA damaging properties of Eclipta alba and Phyllanthus amarus

“…On comparing with tube dilution technique, micro dilution was found to be better that it cleared the leptospires even during the study period of 30 min, which might make this method to be better suited for performing antileptospiral studies. By observing all the above results, the in vitro antileptospiral activity of E. alba and P. amarus was well studied and proved as a best antileptospiral drug [31].…”

Current treatment options for leptospirosis: a mini-review

“…A comparison was made between the activity of the chloroform extract from the leaves of Piper betle and a standard drug. Prabhu et al (2008) evaluated the spirocidal activity of Eclipta alba (commonly known as false daisy, yerba de tago, guntagalagara aaku, Karisalankanni, and bhringraj) extracts using tube dilution and microdilution techniques, demonstrating excellent efficacy against multiple Leptospira species. Additionally, Umamaheswari et al (2010) investigated the spirocidal activity of Seenthil sarkarai and Nilavembu kudineer extracts, revealing significant inhibitory effects against Leptospira, further supporting the potential of natural compounds as therapeutic agents.…”