In Vitro Evaluation of Quercetin Nanocrystals with Different Particle Sizes

Abstract

As one of the most prospective delivery systems of insoluble drugs, nanocrystal has attracted more and more attention from pharmaceutical researchers. Nanoparticles with prominent different particle diameters were obtained to solve the problem of solubility and evaluate the positive effects of quercetin in vitro. The newly prepared nanocrystals were investigated by several methods including dynamic light scattering (DLS) and transmission electron microscopy (TEM). The particle sizes of the quercetin nanocrystals A (quercetin/NCs-A), quercetin nanocrystals B (quercetin/NCs-B) and quercetin nanocrystals C (quercetin/NCs-C) were 3.089±0.162 μm, 677.2±9.7 nm and 169.8± 0.3 nm respectively. The crystalline state of quercetin was detected by X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) analyses and no change was observed after the process of reducing sizes. An accelerated quercetin dissolution rate was achieved through the nanocrystal technique. In conclusion, nanocrystals could significantly increase the equilibrium solubility of quercetin In Vitro. In addition, the particle size also had an important effect on the dissolution behavior of quercetin.

Trial to study the antibacterial effect of quercetin nanoparticles (QUENPS) on drug- resistant E. coli strains in rabbits

“…We observed a significant GPx and SOD depletion that might be a mechanism for the Escherichia coli infection in rabbits, quercetin is able to increased GPX and SOD levels and this effect may also participate in protection against Escherichia coli infection, but for the first time here, we showed that antioxidant potential of quercetin nanoparticles was significantly increased in GPx than quercetin in both normal and infected rabbits. So, nano-sized quercetin particles more effective than quercetin and lead to the efficiency of antioxidant defence systems activity and this in agreement with Zhang et al (2020) who confirmed that nanoparticles with prominent different particle diameters were solved the problem of solubility and evaluate the positive effects of quercetin in vitro.…”

Preparation of caffeic acid grafted chitosan self-assembled micelles to enhance oral bioavailability and antibacterial activity of quercetin

“…Efforts have been made to overcome these challenges, by incorporating QR into various carriers, such as polymeric conjugates ( 14 ), micelles ( 15 ), emulsions ( 16 ), liposomes ( 17 ) and nanocrystals ( 18 ). The modification of QR nanoparticles with silver nanoparticles showed a significant improvement in antibacterial effect against drug-resistant Escherichia coli and Staphylococcus aureus ( 19 ).…”