<i>In vitro</i> Fertilizing Capacity of Deep Frozen Boar Semen Packaged in 0.5 and 5 ml Straws

Córdova, Alejandro; Pérez, Joan Negre; Lleó, Belén; Artiga, Carlos García; Rillo, Santiago Martín

doi:10.1046/j.1439-0531.2001.00296.x

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…Cryopreservation has been reported to affect sperm motility and viability and increase ROS-induced oxidative stress, DNA fragmentation, and apoptosis [13,43,44]. Cryopreservation has also been linked to calcium homoeostasis, acrosome integrity, structural and functional alterations in sperm plasma membranes, which could result in leakage of essential intracellular enzymes such as antioxidants, acrosin, aspartate aminotransferase (AspAT), or energy substrates (i.e., adenosine triphosphate (ATP)), and ultimately lead to cell death [2,45].…”

Section: Discussionmentioning

confidence: 99%

Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing

Dai

Qazi

Ran

et al. 2019

IJMS

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Due to lower farrowing rate and reduced litter size with frozen-thawed semen, over 90% of artificial insemination (AI) is conducted using liquid stored boar semen. Although substantial progress has been made towards optimizing the cryopreservation protocols for boar sperm, the influencing factors and underlying mechanisms related to cryoinjury and freeze tolerance of boar sperm remain largely unknown. In this study, we report the differential expression of mRNAs and miRNAs between fresh and frozen-thawed boar sperm using high-throughput RNA sequencing. Our results showed that 567 mRNAs and 135 miRNAs were differentially expressed (DE) in fresh and frozen-thawed boar sperm. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the majority of DE mRNAs were enriched in environmental information processing such as cytokine-cytokine receptor interactions, PI3K-Akt signaling, cell adhesion, MAPK, and calcium signaling pathways. Moreover, the targets of DE miRNAs were enriched in significant GO terms such as cell process, protein binding, and response to stimuli. In conclusion, we speculate that DE mRNAs and miRNAs are heavily involved in boar sperm response to environment stimuli, apoptosis, and metabolic activities. The differences in expression also reflect the various structural and functional changes in sperm during cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing

Dai

Qazi

Ran

et al. 2019

IJMS

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“…In the present study the IVF is shown as a precise technique to assess freezing procedures of boar semen. In this way, a limited number of factors related with the freezing process have been evaluated by the use of an IVF system (holding time and type of package (Eriksson et al 2000) or volume of straw (Cordova et al 2001, 2002). As conclusion, we can determine that a fast thawing speed had a more positive effect on the fertilizing capacity in vitro of the frozen boar semen.…”

Section: Discussionmentioning

confidence: 99%

Analysis of In vitro Fertilizing Capacity to Evaluate the Freezing Procedures of Boar Semen and to Predict the Subsequent Fertility

Sellés

Gadea

Romar

et al. 2003

Reprod Domestic Animals

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ContentsA porcine in vitro fertilization (IVF) system and seminal quality parameters of frozen-thawed boar semen were used to assess the effectiveness of two different thawing rates of frozen boar semen, and to address the question of whether differences between fertility of ejaculates could be predicted in a limited field trial.In the first experiment, two thawing procedures were analysed (37°C, 30 s; 50°C, 12 s) and no differences in sperm quality were found. However, when the procedure was 50°C, 12 s the IVF results showed a higher number of sperm per penetrated oocyte and a near 10 points higher rate of pronuclear formation.In the second experiment, the fertility results obtained in the limited field trial show to be efficient enough for application in a commercial use, especially for three of the employed boars (fertility ‡80%). In this limited study, the conventional seminal parameters are not accurate enough to discriminate good and bad boars in relation to fertility. On the contrary, parameters of in vitro penetrability are more precise to predict subsequent fertilities.As conclusion, the IVF fertilization system seems to be a good tool to evaluate the quality of frozen-thawed boar semen previous to its commercial way, to verify the bank semen storage quality and a good way to assay new sperm freezing procedures, as it is the more precise evaluating method in estimating the potential fertilizing ability.

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“…For instance, Córdova et al. () found no differences between 0.5‐ and 5‐ml straws in terms of post‐thaw sperm quality, total penetration, monospermy and polyspermy rates, and Eriksson et al. () reported post‐thaw sperm motility was higher when 5‐ml FlatPacks were used.…”

Section: Cooling Rates and Sperm Packagingmentioning

confidence: 99%

Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives

Yeste

2015

Reprod Domestic Animals

112

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ContentsWhile sperm cryopreservation is the best technology to store boar semen for long-term periods, only 1% of all artificial inseminations (AI) conducted worldwide are made using frozen-thawed boar sperm. With the emergence of long-term extenders for liquid storage, the use of cryopreserved sperm in routine AI is less required. However, banks of boar semen contain cryopreserved sperm and planning inseminations in AI centres may benefit from the use of frozen-thawed semen. Therefore, there is an interest in the use of this technology to preserve boar sperm. In this regard, although the first attempts to cryopreserve boar semen date back to the seventies and this technology is still considered as optimal, some relevant improvements have been made in the last decade. After giving a general picture about boar sperm cryodamage, the present review seeks to shed light on these recent cryopreservation advances. These contributions regard to protein markers for predicting ejaculate freezability, sperm selection prior to start cryopreservation procedures, additives to freezing and thawing extenders, relevance of the AI-technique and insemination-toovulation interval. In conclusion, most of these progresses have allowed counteracting better boar sperm cryodamage and are thus considered as forward steps for this storage method. It is also worth noting that, despite being lower than fresh/ extended semen, reproductive performance outcomes following AI with frozen-thawed boar sperm are currently acceptable.

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In vitro Fertilizing Capacity of Deep Frozen Boar Semen Packaged in 0.5 and 5 ml Straws

Cited by 9 publications

References 26 publications

Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing

Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing

Analysis of In vitro Fertilizing Capacity to Evaluate the Freezing Procedures of Boar Semen and to Predict the Subsequent Fertility

Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives

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