In vitrogermination and viability of pollen grain of coconut accessions

Abstract

-Storage as a means of maintaining the pollen viability is important for the preservation of the genetic variability, facilitates the exchange of germplasm and greatly contributes to the generation of variability obtained from artificial crosses, increasing the efficiency of breeding programs. The objective of this study was to select different culture media for the in vitro germination of pollen grain of dwarf and tall coconut accessions, as well as to determine the viability of pollen grain at room temperature conditions. For this purpose, Brazil Green Dwarf (BGD) and Brazilian Tall (BRA) pollen grains derived from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands Sergipe were used. To evaluate the effect of different culture media on the in vitro germination of pollen grains of anão verde do Brasil de Jiqui (AVeBrJ) and gigante do Brasil Praia do Forte (GBrPF) accessions, they were inoculated on to Petri dishes containing 2 ml of culture media. The pollen viability was assessed by staining with 1% acetic carmine and in vitro germination at 0, 24, 48 and 72 hours. The culture medium of Lora is suitable to assess the in vitro germination of pollen grain of the AVeBrJ and GBrPF accessions. The pollen grain of the AVeBrJ accession showed intermediate viability (66.87%) at room temperature up to 23.14 hours by in vitro germination. The pollen grain of the GBrPF accession showed high viability, above 70%, at room temperature up to 120 hours by in vitro germination.

Viability of pollen grains of tetraploid banana

“…Each species requires a specific protocol to obtain a high germination rate. The success of in vitro germination depends on several factors, such as species, nutritional state, season of the year, time of collection, photoperiod, temperature, collection method, incubation period and culture medium, besides adjustments in the composition of the medium for each species (Stanley and Linskens 1974;Sharafi 2010;Moura et al 2015).…”

In vitro viability of pollen grain of fiji dwarf coconut accession

“…Two liquid culture media were evaluated for in vitro germination of Fiji dwarf coconut pollen. The Lora culture medium (Lora et al, 2006) and the Armendariz culture medium (Armendariz et al, 2006) were selected due to the previous results published for coconut varieties (Armendariz et al, 2006;Machado et al, 2014;Moura;Machado;Ledo, 2015). Both media compositions are described in Table 1.…”

“…The second, was completely randomized in a 4 x 2 x 2 factorial scheme (four sucrose concentrations x two light conditions x two incubation periods) with four repetitions, each composed of a Petri The highest pollen germination percentage (47.75%) in Lora culture medium at room temperature (28 ºC) also was reported by Machado et al (2014) in CRD (Cameroon red dwarf) coconut, where a pollen germination percentage of 49.64% (Figure 1). Furthermore, the Lora culture medium has been shown to be suitable for evaluating of in vitro pollen germination of the BGD (Brazilian Green Dwarf of Jiqui) and BT (Brazilian Tall) coconuts (Moura;Machado;Ledo, 2015). However, at the other incubation temperature levels (22, 25 and 31 °C) the Armendariz culture medium showed higher in vitro pollen germination percentages ranged from 33.3 to 34.46% than the Lora culture medium.…”

“…Previous studies indicate variation of in vitro pollen germination responses as a function of the culture medium and the coconut genotype (Armendariz et al, 2006;Karun et al, 2006;Karun;Sajin, 2010;Machado et al, 2014;Moura;Machado;Ledo, 2015). Currently, there is no information available for in vitro pollen germination of Fiji dwarf coconut.…”

Viabilidad y longevidad del polen en genotipos de limón mexicano estimada mediante germinación in vitro

“…La viabilidad del polen y su poder germinativo en laboratorio se puede determinar mediante a): Pruebas de germinación in vitro (Moura et al, 2015;Shekari et al, 2016); b) medición de la actividad enzimática (Gozlekci et al, 2011;Soares et al, 2013;Demir et al, 2015); y c) la tinción del citoplasma del grano de polen (Maiti y Rodríguez, 2015;Baswal et al, 2015). Sin embargo, Soares et al (2013); Kundu et al (2014); Shekari et al (2016), coinciden que la germinación in vitro es más confiable ya que los métodos de tinción pueden sobreestimar la viabilidad del polen.…”