<i>In Vitro</i> Micropropagation of Industrially and Medicinally Useful Plant <i>Aloe trichosantha</i> Berger Using Offshoot Cuttings

Hailu, Atsbeha; Sbhatu, Desta Berhe; Abraha, Haftom Baraki

doi:10.1155/2020/3947162

Cited by 5 publications

(17 citation statements)

References 24 publications

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“…In vitro propagation media enriched with 0.20/0.20 mg/L, 4.0/0.20 mg/L, 0.50/0.50 mg/L, and 0.20/0.20 mg/L BAP/NAA have been effective for initiating growth in A. percrassa Tod. [ 7 ], A. vera L. [ 11 ], A. trichosantha Berger [ 12 ], and A. adigratana Reynolds [ 13 ], respectively. BAP and NAA are often used for initiating explants of many aloe species.…”

Section: Resultsmentioning

confidence: 99%

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In vitro micropropagation of Aloe elegans Tod. using offshoot cuttings

Welehaweria,

Sbhatu

2023

BMC Res Notes

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Objective Aloe elegans Tod. is an ecologically, environmentally, medicinally, and commercially useful aloe species in Ethiopia and Eritrea. Unfortunately, it is highly threatened due to industrial and urban expansion and traditional mining and agricultural activities. As a consequence, it is included in the IUCN List of Threatened Species since 2013. The plant is getting thinly populated in many parts of the Tigrai floristic region since it is being exploited for traditional and commercial purposes. Therefore, this study was aimed to develop a reproducible, large-scale micropropagation protocol using offshoot cuttings in Murashige and Skoog (MS) media enriched with plant growth regulators (PGRs). Results Sterilized explants cultured in full-strength MS media enriched with 0.25 mg/L benzyl amino purine (BAP) and 0.10 mg/L naphthaleneacetic acid (NAA) resulted in 100% healthy and live (i.e., initiated) explants after four weeks of initiation study. Unsupplemented initiation media (control) yielded only 14.3% initiated explants. The initiated explants were tested for their shooting response to produce microshoots by incubating in different concentrations and combinations of BAP and NAA for four weeks. Fewer days to shooting (13.0 ± 1.0 days), higher mean shoot number (5.0 ± 1.0), and higher mean shoot length (9.20 ± 2.35 cm) were observed with 1.0/0.50, 1.0/0.25, and 1.0 /0.50 mg/L BAP/NAA combinations, respectively. The rooting responses of the microshoots toward producing plantlets were also tested by incubating them in half-strength MS media enriched with different concentrations of NAA and indole-3-butyric acid (IBA) for four weeks. Fewer mean days to rooting (12.0 ± 1.0 days), higher mean root number (8.0 ± 4.0), and higher mean root length (7.53 ± 3.03 cm) were observed in MS media enriched with 0.75, 0.75, and 1.25 mg/L IBA, respectively. The responses of A. elegans plantlets to primary (in greenhouse) and secondary (in nursery shade and direct sunlight) acclimatization in coco peat, composted soil, and manured soil media were high – with survival percentages of 87.5–97.8% in three to four weeks.

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Section: Resultsmentioning

confidence: 99%

“…1 ( a )). Many other studies have reported shooting in A. adigratana Reynolds, A. percrassa Tod., A. trichosantha Berger, and A. vera L. within two weeks in MS media enriched with 1.0 mg/L BAP in combination with auxins (such as IAA and NAA) [ 7 , 12 – 14 ].…”

Section: Resultsmentioning

confidence: 99%

In vitro micropropagation of Aloe elegans Tod. using offshoot cuttings

Welehaweria,

Sbhatu

2023

BMC Res Notes

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“…Several past studies on the Aloe vera plant concentrated on propagation using stems and shoot tips, rooted easily with the formation of whole plants (Hailu et al, 2020). It seems likely that certain obstacles faced by callus formation than the complexities challenged its growth histologically, despite providing the required media and supporting it with reasonable growth regulator types and concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…Samples remained in the culture room in the same condition previously mentioned. Cutting out Aloe vera vegetative branches obtained from cultivation of the apical shoot and stems used a sharp scalpel, with their bases planted upright in 25 ml of agar-solidified MS medium supplemented with 1.0 mg L -1 NAA (Hailu et al, 2020).…”

Section: Culture Of Apical Shootmentioning

confidence: 99%

Propagation Protocol of the Medicinal Plant – Aloe Vera Using Tissue Culture

AL-NEMA¹,

ABDULLAH²

2023

SABRAOJBG

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Aloe vera is one of the most popular cactus-type plants in the global market due to its widespread uses in pharmaceutical, cosmetic, food, and decorative purposes. The present study derived callus cultures from the Aloe vera plant leaves, then reproduced on agar-solidified MS medium from June to December 2021 at the University of Mosul, Iraq. Results revealed that the MS medium + 3.0 mg L-1 benzyl adenine (BA) proved suitable for induction of leaf callus up to 85%, while the MS medium supplement with 1.0 and 2.0 mg L-1 BA reached 70%. The MS medium with 1.0 mg L-1 BA showed the best results for growing apical shoots of A. vera plants and producing vegetative branches. The formation of roots emerged within two weeks after placing them on the rooting medium. The shoots regenerated from the growing apices and were rooted easily in agar-solidified MS medium. The obtained plants attained successful acclimatization in terms of their growth and length, afterward, transferred to the peat-moss mixture.

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“…However, the optimum concentration and/or combination of PGRs varies from plant to plant and from genotype to genotype (Molsaghi et al, 2014). The individual effects of various PGRs in efficient micropropagation of Aloe were reported in several studies (Lavakumaran and Seran, 2014;Hailu et al, 2020). Interestingly, 6-benzylaminopurine (BAP) alone was proved to be more effective than the combination of BAP and naphthaleneacetic acid (NAA) for in vitro propagation of A. vera (Danial et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Auxin and cytokinin synergism in micropropagation for mass production of Aloe vera

Yasmin¹,

Hasan²,

Hossain³

et al. 2022

bta

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Aloe vera [Aloe vera (L.) Burm. f.] is considered a valuable medicinal plant worldwide due to its remarkable beneficial effects on human health. However, challenges in A. vera propagation hinder meeting the increasing demand in the health and beauty sectors. As an alternative method, in vitro propagation is crucial for the mass production of Aloe plants, which is a rapid method as well. Therefore, the present study aimed to establish an efficient micropropagation protocol for A. vera by in vitro optimization of the effect of different plant growth regulators (PGRs).For shoot proliferation, sterilized explants were inoculated on the Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) and thidiazuron (0.5, 1.0, 2.0, and 4.0 mg/l) in combination with 0.5 mg/l naphthaleneacetic acid (NAA). Subsequently, indole-3-butyric acid (IBA) (1.0, 2.0, and 3.0 mg/l) was used for root induction. It was found that the explants cultured on the MS medium supplemented with 4.0 mg/l BAP + 0.5 mg/l NAA showed the highest percentage of response (90 ± 1.29) for shoot induction within the minimum number of days (5 ± 0.33). The highest number of shoots (2.7 ± 0.36) and length of shoots (4.7 ± 0.42 cm) per explant were also observed with the same concentration of PGRs. However, the highest number of roots (3.2 ± 0.57), length of roots (5.67 ± 0.21 cm), and root induction (80 ± 1.97 %) were noticed within the minimum number of days (11 ± 0.79) on the MS medium supplemented with 1.0 mg/l IBA. Thus, the proposed method is a quick and effective approach for the mass propagation of A. vera with appropriate dosages of auxins and cytokinins, which may allow meeting the increasing commercial demand.

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In Vitro Micropropagation of Industrially and Medicinally Useful Plant Aloe trichosantha Berger Using Offshoot Cuttings

Cited by 5 publications

References 24 publications

In vitro micropropagation of Aloe elegans Tod. using offshoot cuttings

In vitro micropropagation of Aloe elegans Tod. using offshoot cuttings

Propagation Protocol of the Medicinal Plant – Aloe Vera Using Tissue Culture

Auxin and cytokinin synergism in micropropagation for mass production of Aloe vera

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