<i>In Vitro</i>Plant Regeneration from Turkish Grasspea (<i>Lathyrus Sativus</i>L.) Using Immature Zygotic Embryo Explant

Kendir, Hayrettin; Sahin-Demirbag, Nurdan; Khawar, Khalid Mahmood; Özcan, Sebahattin

doi:10.1080/13102818.2009.10817634

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…Whereas, Sancak et al (12) found that immature cotyledons appeared to produce the highest frequency of shoot regeneration on 20 µM 6-benzylaminopurine (BA) and 2.5 µM α-aphthaleneacetic acid (nAA)-containing medium, whereas, shoot regeneration on immature embryo axes was best achieved on a medium supplemented with 5 µM each of BA and nAA. Similarly, in vitro culture response of cotyledonary node of V. narbonensis was found to be influenced by the concentration of plant growth regulators in the medium in agreement with Kendir et al (6). the results are also in agreement with taha and Farncis (14) and erdogan et al (4), who obtained high shoot regeneration from V. ervilia using immature embryo and cotyledon explants on MS medium using various concentrations of tDZ.…”

supporting

confidence: 87%

In VitroPlant Regeneration from Hungarian Vetch (Vicia PannonicaCrantz) Using Cotyledonary Node Explants

Sahin-Demirbag

Kendir

Khawar

et al. 2008

Biotechnology & Biotechnological Equipment

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supporting

confidence: 87%

In VitroPlant Regeneration from Hungarian Vetch (Vicia PannonicaCrantz) Using Cotyledonary Node Explants

Sahin-Demirbag

Kendir

Khawar

et al. 2008

Biotechnology & Biotechnological Equipment

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“…On the other hand tissue culture and biotechnological research can help in the efforts to produce plants free of this neurotoxin. In general, grain legumes, including Lathyrus species, are very recalcitrant to regeneration in vitro and limited numbers of literatures are available on tissue culture and genetic transformation studies (Kendir et al, 2009;Sahin-Demirbag et al, 2008;Khawar et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

In vitro Regeneration of Grass Pea (Lathyrus sativus L.)

Saha

Afrin

Mohiuddin

et al. 2016

Jahangirnagar Univ. J. Biol. Sci.

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In vitro regeneration protocol for grass pea (Lathyrus sativus L.) was optimized using different concentrations and combinations of growth regulators. Direct shoot regeneration obtained through shoot organogenesis from different explants of grass pea cultured on MS medium supplemented with Gamborg B5 vitamin containing 6-benzylaminopurine (BAP), Thidiazuron (TDZ) and ?-naphthalene acetic acid (NAA). Highest percentage of shoots were obtained at 4.0 mg/l of BAP on nodal explants. Stunted multiple shoots were developed from nodal explants while 1.5 mg/l TDZ was used. About 56% of direct shoots were also obtained, while the combination of BAP (4.0 mg/l) and NAA (0.5 mg/l) were used. Regenerated plantlets were rooted most effectively (40%) in rooting medium containing half strength of MS basal medium containing 1.0 mg/l NAA. Well rooted plantlets were further successfully acclimatized to ambient humidity level and grown in controlled environment until hardening.Jahangirnagar University J. Biol. Sci. 4(2): 1-8, 2015 (December)

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“…Barna and Mehta, (1995) somatic embryogenesis was obtained from immature leaflets and nodal segments by using 2,4-D in the induction medium. Kendir at al., (2009) in the study used immature zygotic embriyos of grass pea (Lathyrus sativus L.), the TDZ (with ascorbic acid) was more potent for axillary shoot regeneration compared to BAP-NAA (with ascorbic acid) with the highest shoot regeneration on MS medium containing 0.45 mg/l TDZ. The best rooting was achieved on MS medium containing 0.90 mg/l NAA.…”

Section: Somatic Embriyogenesismentioning

confidence: 99%

In vitro regeneration techniques in the grass pea (Lathyrus sativus L.) plant

Çatal

Bakoğlu

2018

Eurasian Journal of Forest Science

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The Lathyrus genus is in the legumes family with annual or perennial species number of 160. There are 58 species naturally grown in Turkey, 18 of them are endemic. In Rize province, 8 species were identified, 2 of them were endemic. In the world, Lathyrus species are evaluated in the animal feed as green grass, hay and grain feed, fertilization of soil as a green manure plant and human nutrition as food grain legume plant. The cultivation of Lathyrus species are very rare in our country and are generally used in animal feeding and in small quantities in human nutrition. Grass pea (Lathyrus sativus L.) plant is the most used species in the world, because it is resistant to adverse soil conditions, drought and flooding. Various tissue culture methods are used to develop and reproduce this species. Plant tissue culture is being applied both in the development of new varieties and genetic changes in existing varieties, and in the production of species which are difficult to reproduce and protect of the disappearing species. The basic system used in plant tissue culture processes and genetic improvements is plant regeneration. In this review, some studies related to the in vitro regeneration techniques of the grass pea plants have been put together and the techniques used in regeneration have been evaluated.

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In VitroPlant Regeneration from Turkish Grasspea (Lathyrus SativusL.) Using Immature Zygotic Embryo Explant

Cited by 5 publications

References 15 publications

In VitroPlant Regeneration from Hungarian Vetch (Vicia PannonicaCrantz) Using Cotyledonary Node Explants

In VitroPlant Regeneration from Hungarian Vetch (Vicia PannonicaCrantz) Using Cotyledonary Node Explants

In vitro Regeneration of Grass Pea (Lathyrus sativus L.)

In vitro regeneration techniques in the grass pea (Lathyrus sativus L.) plant

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