In vitroPlantlet Regeneration from Leaf and Stem Calluses ofRauwolfia tetraphylla(R. canescens) and Confirmation of Genetic Fidelity of Plantlets Using the ISSR-PCR Method

Rohela, Gulab Khan; Bylla, Prasad; Kota, Srinivas; Abbagani, Sadanandam; Chithakari, Ravi; Reuben, T. Christopher

doi:10.1080/10496475.2012.741056

Cited by 17 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our earlier studies, we have used single cytokinin (TDZ) for regeneration of shoot buds from leaf and stem based callus and obtained maximum frequency of shoot regeneration (12.0±0.28) from stem based callus at 2.27 μ M/l concentration of TDZ [25]. Now through this research paper we are reporting a standardized protocol with much higher frequency of shoot regeneration from the friable callus of leaf and stem explants on MS media supplemented with individual (Figures 2 and 3) and combination of cytokinins (TDZ and BAP) (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

“…Thidiazuron (TDZ) was used by several researchers in regeneration studies of medicinal plants with higher frequency of shoot let production from friable callus [36, 37]. In our earlier studies we have induced low frequency of multiple shoots from stem callus of R. tetraphylla on TDZ amended media [25].…”

Section: Resultsmentioning

confidence: 99%

“…Even though there are some reports about its multiplication through nodal, shoot tip cultures [23] and in vitro flowering [24], but there is scanty information about its regeneration through callus based indirect regeneration protocols. In our previous report, we have used single type of cytokinin (TDZ) in regenerating the low frequency of multiple shoots from leaf and stem based callus [25]. Now, in this study we are reporting a protocol for its conservation through high frequency of plantlet production by using combination of cytokinins amended media.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Rohela

Jogam

Bylla

et al. 2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Rauwolfia tetraphylla L. is an important medicinal plant species which is well known for its pharmaceutically important alkaloids. In the present study, we are reporting about its conservation by in vitro clonal multiplication through the standardized protocol of indirect regeneration by using leaf and stem based callus and assessment of genetic fidelity of acclimated plantlets by start codon targeted (SCoT), inter simple sequence repeats (ISSR), and randomly amplified polymorphic DNA (RAPD) marker based analysis. Initially friable callus was induced in maximum amounts (378.7, 323.8, and 412.8 in mg) from leaf, root, and stem explants on Murashige and Skoog (MS) media supplemented with 5.0 mg/L, 3.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) and 5.0 mg/L of naphthalene acetic acid (NAA), respectively. Shoot regeneration with the maximum number of shoot buds (25 and 20) was obtained from leaf and stem calluses on MS media supplemented with TDZ (0.25 mg/L) + BAP (2 mg/L). The regenerated shoots were rooted successfully with maximum rooting percentage of 98.0 on full strength MS media amended with IAA (1.0 mg/L) and IBA (1.0 mg/L). The regenerated plantlets were hardened using 2:1 ratio of sterile garden soil and sand, followed by acclimatization in field conditions with 86% of survival. SCoT, ISSR, and RAPD primers based polymerase chain reaction (PCR) analysis was carried out to check possible genetic variations in micro propagated plants in comparison with mother plant. Among the ten SCoT (S), ISSR (R), and RAPD (OPA) primers used, S2, R10, and OPA3 has given good amplification with scorable DNA bands. The results revealed that the regenerated plants did not have any polymorphism with mother plant. Hence, the in vitro regenerated R. tetraphylla plantlets were confirmed as true-to-type.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Rohela

Jogam

Bylla

et al. 2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rohela et al [23][24][25] has used the MS+TDZ+BAP combination for the in vitro shoot regeneration from calli derived from leaf and stem explants of Rauwolfia tetraphylla L.…”

Section: Results and Discussion:-mentioning

confidence: 99%

In Vitro Micropropagation of Stachytarpheta Jamaicensis L.VAHL., an Ethnomedicinal Plant and Confirmation of Genetic Fidelity of the Plantlets Using Rapd Markers.

Korra¹,

Prasad²,

Rohela³

et al. 2017

IJAR

View full text Add to dashboard Cite

“…R. tetraphylla alkaloids which are in use of cardiovascular and psychiatric treatments (Anitha and Kumari, 2006), alkaloids has anti-hypertensive activity (Faisal et al, 2005;Rohela et al, 2013). The root is also used to promote uterine contraction and it is recommended for use during child birth in difficult ones (Villar et al, 1998;Tona et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening and Antimicrobial Activity of Leaf, Stem, Root and their Callus Extracts in Rauwolfia tetraphylla

Rohela¹,

Bylla²,

Korra³

et al. 2016

IJAB

Self Cite

View full text Add to dashboard Cite

Phytochemical screening and Antimicrobial studies were carried out in a medicinally important plant species, Rauwolfia tetraphylla. The aim of the present study was to screen leaf, stem and root extracts and their callus (NaCl stressed & unstressed) extracts of R. tetraphylla (24 months old) for the qualitative analysis of phytochemicals like alkaloids, flavonoids, saponins, tannins and gums and mucilages and to study antibacterial and antifungal activities in these extracts. Phytochemical screening revealed that Alkaloids are major phytochemicals in leaf, stem and root extracts of R. tetraphylla (24 months old). In antimicrobial activity studies, maximum bacterial growth inhibition zone (25.0 ± 2.4 mm) was observed in methanol based leaf extracts against Staphylococcus aureus and maximum fungal growth inhibition zone (22.0 ± 2.1 mm) was observed in Fusarium oxysporum in methanol based root extracts. In NaCl stressed callus extracts both antibacterial and antifungal activities were observed but the results were not comparable to extracts of in vivo plant based leaf, stem and roots.

show abstract

In vitroPlantlet Regeneration from Leaf and Stem Calluses ofRauwolfia tetraphylla(R. canescens) and Confirmation of Genetic Fidelity of Plantlets Using the ISSR-PCR Method

Cited by 17 publications

References 20 publications

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

In Vitro Micropropagation of Stachytarpheta Jamaicensis L.VAHL., an Ethnomedicinal Plant and Confirmation of Genetic Fidelity of the Plantlets Using Rapd Markers.

Phytochemical Screening and Antimicrobial Activity of Leaf, Stem, Root and their Callus Extracts in Rauwolfia tetraphylla

Contact Info

Product

Resources

About