<i>In vitro</i> polydeoxyribonucleotide effects on human pre‐adipocytes

Raposio, Edoardo; Guida, Chiara; Coradeghini, Rosella; Scanarotti, Chiara; Parodi, Alessandro; Baldelli, Ilaria; Fiocca, Roberto; Santi, Pierluigi

doi:10.1111/j.1365-2184.2008.00547.x

Cited by 46 publications

(36 citation statements)

References 54 publications

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“…Recently, it has been reported by Banas et al [2007] that TTR is expressed in ADSC CD105+ differentiated into hepatic lineage. In our previous study [Raposio et al, 2008], we verified that MTT test, DNA assay and CFSE staining were suitable analyses to evaluate cell proliferation in undifferentiated ADSC, in accordance with other data in the literature [Hemmrich et al, 2006;Liu et al, 2008;Zaminv et al, 2008]. So, we apply the above analyses to evaluate the proliferation rate of HA and HB cells.…”

Section: Discussionmentioning

confidence: 50%

“…Carboxyl fluorescein succinimidyl ester (CFSE) staining was similarly performed as previously reported by Raposio et al [2008]. Briefly, 1 day before seeding, HepG2 cells, undifferentiated ADSC, HA and HB at the end of differentiation were washed with DPBS and incubated for 10 min in the dark at 37 ° C and 5% CO 2 with 5 M CFSE solution in DPBS; successively, the cells were washed with DPBS added with 10% FBS [Urbani et al, 2006].…”

Section: Carboxyl Fluorescein Succinimidyl Ester Stainingmentioning

confidence: 99%

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A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells

Coradeghini

Guida

Scanarotti

et al. 2009

Cells Tissues Organs

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Human adipose-derived stem cells possess a lot of stem cell characteristics, so they may be considered a source of stem cell population. On the basis of that, we have investigated the hepatic potential of adipose-derived stem cells, obtained from liposuction, following two differentiation protocols. In the first procedure, medium was supplemented with epidermal growth factor (EGF), basic fibroblast growth factor, hepatocyte growth factor (HGF) and nicotinamide; the second involved the addition of factors such as dexametasone, EGF, insulin-transferrin-sodium selenite, HGF, dimethyl sulfoxide and oncostatin. In parallel, we carried out our study in the Hep G2 cell line, as human hepatic differentiated in vitro model. Immunocytochemical analysis and RT-PCR were performed using hepatic markers to evaluate cell differentiation. DNA content, MTT test and carboxyl fluorescein succinimidyl ester staining were carried out to evaluate cell proliferation. We reported the evidence of basal hepatic marker in undifferentiated adipose-derived stem cells, which confirmed their multipotency. A strong expression of albumin and α-fetoprotein was observed in hepatic-induced adipose-derived stem cells following both differentiation procedures. Morphological aspects of the two types of hepatic adipose-derived stem cells were alike. Proliferation index suggested that the first differentiation procedure promoted better growth than the second. These preliminary findings suggest adipose-derived stem cells may be induced into hepatic lineage, and the most significant difference between the two standard differentiation procedures concerns proliferation rate. This aspect is to be considered when adipose-derived stem cells are employed in research and clinical studies.

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Section: Discussionmentioning

confidence: 50%

Section: Carboxyl Fluorescein Succinimidyl Ester Stainingmentioning

confidence: 99%

A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells

Coradeghini

Guida

Scanarotti

et al. 2009

Cells Tissues Organs

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“…PDRN is helpful for normal matrix formation by shortening the healing period through its prompt tissue regeneration and wound healing and by promoting the creation of collageneous/ noncollageneous protein. Also, it is characterized by being effective in tissue regeneration by promoting cell regeneration in cellular stage [9][10][11][12][13][14] . In 2008, an autogenous bone graft material was developed from extracted teeth, and subjected to many basic and clinical studies.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Autogenous Tooth Bone Graft Combined with Growth Factor: Prospective Cohort Study

Ahn¹,

Kim²,

Yun³

et al. 2013

Journal of Korean Dental Science

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“…However, this is not the only function of lipofilling. Fat is an active and dynamic tissue composed of several different cell types, including adipocytes, fibroblasts, smooth muscle cells, endothelial cells, and adipogenic progenitor cells called pre-adipocytes [30], [31], [32]. Stem cells isolated from lipoaspirates have demonstrated a broad in vitro adipogenic, chondrogenic, osteogenic, and myogenic lineage commitment [33], [34] as well as differentiation into pancreatic cells, hepatocytes, and neurogenic cells [35], [36], [37].…”

Section: Efficacy Of Lipofillingmentioning

confidence: 99%

Autologous fat transplantation for breast reconstruction: A literature review

Simonacci

Bertozzi

Grieco

et al. 2016

Annals of Medicine &Amp; Surgery

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IntroductionThe use of autologous fat transplantation to correct volume and contour defects, scars, and asymmetry after breast cancer surgery has increased over the past 20 years. Many developments and refinements in this technique have taken place in recent years, and several studies of the safety of lipofilling in the breast have been published.Presentation of caseWe performed a literature review of this technique, highlighting the crucial role of lipofilling in breast cancer reconstruction.DiscussionThe efficacy of the fat graft transplantation depends on the experience and the technique used by the surgeon. The ASCs (adipose-derived stem cells) contained in the fat graft has proven to be crucial for breast reconstruction by mean the regeneration of tissue, through the chemotactic, paracrine, and immunomodulatory activities and their in situ differentiation.ConclusionThe role of lipofilling for breast reconstruction could be more significant with the application of the findings of experimental research on tissue engineering and ASCs.

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In vitro polydeoxyribonucleotide effects on human pre‐adipocytes

Abstract: PDRN seemed to promote proliferation of human pre-adipocytes at both passages, but cell population growth increased in pre-adipocyte at P16, after 9 days as compared to control. Our data suggest that PDRN could act as a pre-adipocyte growth stimulator.

Cited by 46 publications

References 54 publications

A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells

A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells

Effectiveness of Autogenous Tooth Bone Graft Combined with Growth Factor: Prospective Cohort Study

Autologous fat transplantation for breast reconstruction: A literature review

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