In vitro probiotic evaluation of potential antioxidant lactic acid bacteria isolated from idli batter fermented with Piper betle leaves

Abstract

Lactic acid bacteria isolated from food sources can be introduced as probiotics because of their healthpromoting and nonpathogenic characteristics. Eight lactic acid bacteria from idli batter fermented with Piper betle leaves were selected to screen the antioxidant potential and biofilm-forming ability. Five isolates exhibited good antioxidant potential and biofilm formation and showed antagonistic activity against the pathogenic biofilm-forming Staphylococcus aureus. Further, in vitro probiotic properties of five isolates exhibited excellent acid tolerance, bile tolerance, simulated gastrointestinal juice tolerance, auto-aggregation and co-aggregation and hydrophobicity and showed resistance to antibiotics such as ciprofloxacin, nalidixic acid, norfloxacin and colistin. Isolates also showed positive bile salt hydrolase activity, cholesterol assimilation, b-galactosidase production and mucin binding ability. The isolate KJBB10 exhibited good adhesion and pathogen exclusion percentage with human colon cancer cells (HCT-15 and HT-29). Hence, the isolates KJBB10, KJBB56 and KJBC06 can be used as beneficial probiotic starter cultures for the formulation of functional foods. Reducing activity assayReducing activity of ICFE of potent isolates was assayed by the method of Oyaizu (1986). 0.5 mL of 1% potassium ferricyanide was mixed with the same In vitro probiotic evaluation of LAB V. Sadishkumar and K. Jeevaratnam 330 * indicates the significance of result at P ≤ 0.001. ** indicates the significance of result at P ≤ 0.05. *** indicates the significance of result at P ≤ 0.10 using one-tailed hypothesis. † Ascorbate inhibition percentage. ‡ Expressed in equivalent to cysteine (lg). § Hydroxyl radical scavenging percentage. ¶ Expressed as % DPPH scavenging = [1-(A 515 of sample/A 515 of control)] 9 100. In vitro probiotic evaluation of LAB V. Sadishkumar and K. Jeevaratnam * Indicates the significance of result at P ≤ 0.001. ** Indicates the significance of result at P ≤ 0.05. *** Indicates the significance of result at P ≤ 0.10 using one-tailed hypothesis. † Time delay for bile tolerance was calculated as time taken to increase OD 560 by 0.3 units. ‡ Expressed in %. § Expressed in miller units.

Screening of lactic acid bacteria isolated from fermented Cornus officinalis fruits for probiotic potential

“…Assaying the ability of the bacterial adhesion to hexadecane is one of the qualitative approaches to estimate the ability of isolates to adhere to the epithelial cells (Sadishkumar & Jeevaratnam, ). In our study, the highest hydrophobicity percentage (20.35%) was exhibited by L. plantarum 3711, which was significantly higher than LGG (15.11%, Table ) ( p < .05).…”

Review of The Effectiveness of Plant Media Extracts in Barium Hexaferrite Magnets (BaFe12O19)

“…In recent years betel leaf extract has been researched and successfully synthesized in Fe 2 O 3 and silver and gold nanoparticles as a reducing agent and stabilizer of material synthesis (Shejawal et al, 2020). Piper betel Linn can absorb air pollutant particles from formaldehyde and benzene compounds (Sadishkumar and Jeevaratnam, 2016). Atiya conducted a cell culture study for anticancer activity isolated from chloroform extract of Piper betle Linn leaves as cytotoxic activity against human oral cancer cell lines (Atiya, 2017), Misra et al, 2018 conducted research on betel leaf modi ed with super magnetic iron oxide as a magnetic nano sorbent to remove drugs from aqueous solutions such as carbamazepine.…”

Effect of simulated acidic and salty fermentation conditions on kinetic growth parameters and probiotic potential of Lactobacillus acidipiscis and Lactobacillus pentosus

“…The auto-aggregation and the hydrophobicity were carried out as reported (Sadishkumar & Jeevaratnam, 2017). A 24 h culture of each strain cultured in the conditions aforementioned was harvested by centrifugation (10 000 × g, 10 min, 4°C) and washed twice with phosphate-buffered saline (PBS).…”