<i>In vitro</i> Production of Neurologically active Phytoconstituents from an elite Chemovariant of <i>Centella asiatica</i> (L.), identified from south Indian Populations

Raju, D. C. S.; Varghese, Reji Joseph; Biji, Nancy

doi:10.5958/0974-360x.2017.00366.3

Rese. Jour. of Pharm. and Technol.

2017

DOI: 10.5958/0974-360x.2017.00366.3

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In vitro Production of Neurologically active Phytoconstituents from an elite Chemovariant of Centella asiatica (L.), identified from south Indian Populations

D. C. S. Raju¹,

Reji Joseph Varghese²,

Nancy Biji³

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Cited by 4 publications

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“…The active compounds with low solubility in water will affect the bioavailability of active compounds in the body. To overcome this problem, nanoparticles of chitosan TPP C. asiatica extract can be developed to increase active extract compounds' bioavailability [2][3][4][5][6][7][8][9][10][11][12] .…”

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confidence: 99%

Synthesis and Optimization of Nanoparticle Chitosan-Tripolyphospate Centella asiatica using Ionic Gelation Method with Difference Sonification Time

Muchtaromah,

Budi Minarno,

Annisa

et al. 2023

RJPT

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Nanoparticles from natural polymer materials chitosan are widely applied in drug delivery systems because of their unique properties, such as biocompatible, biodegradable, mucoadhesive, and increased permeation. This study aimed to synthesize chitosan nanoparticles Centella asiatica. The method used is ionic gelation using the crosslinker polyanion tripolyphosphate (TPP) with a difference in sonification time: 90, 120, 150 minutes. The difference in sonification to obtain the preparation conditions that can produce particles under 200nm with a good level of dispersion and stability, a study on the effect of sonification time on chitosan nanoparticles physical characteristics is performed. The PSA analysis result showed that the average particle size of C.asiatica extracts at 90, 120, 150 minutes sonication was 286.2nm, 269.2nm, 299.1nm. The results of the examination using FTIR showed that the C. asiatica extract had N-H and P = O groups, which meant that the ammonium ion had interaction of chitosan with the polyanion of TPP and C. asiatica extract. N-H absorption of pure chitosan shifted from 1597.06cm-1 to 1640.56cm-1, indicating a crosslinking between ammonium ions in chitosan and phosphate ions from TPP and C. asiatica extract. The formulation of using XRD showed that the amorf form of nanoparticle C. asiatica extracts is supported by particle morphology imaged using SEM. Based on the results obtained, the synthesis of nanoparticles C. asiatica extract in this study has been successful.

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mentioning

confidence: 99%

Synthesis and Optimization of Nanoparticle Chitosan-Tripolyphospate Centella asiatica using Ionic Gelation Method with Difference Sonification Time

Muchtaromah,

Budi Minarno,

Annisa

et al. 2023

RJPT

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In Silico Study of Centella asiatica Derivatives as Antioxidant: Enhancer of Superoxide Dismutase and Glutathione Peroxidase Activity

Legiawati

Fadilah

Bramono

et al. 2023

RJPT

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Superoxide dismutase (SOD) and glutathione peroxidase (GPx) are both parts of the enzymatic line in the antioxidant framework which changes anion superoxide to a more stable compound like oxygen (O2) and hydrogen peroxide (H2O2). Centella asiatica significantly shows antioxidant activity in several studies with comparable activity to ascorbic acid and butylated hydroxytoluene. This study assessed the antioxidant properties of Centella asiatica by studying its interaction with SOD and GPx. Active compounds of Centella asiatica were selected based on their interactions with SOD and GPx to determine which compounds reacted significantly. Significant interaction in the docking study was determined by the binding energy of each compound to the enzymes. Active compound of Centella asiatica had been proven to interact with both SOD and GPx. SOD bound with asiaticoside binding energy -10.2310 kcal/mol and madecassic acid binding energy -9.0518 kcal/mol. Based on protein residue, the majority of the protein bods into Gln 118. Both asiaticosside and madecassic acid bound to Gln118. Madasiatic acid and asiaticoside are bound to GPx with the lowest binding energy ligand, respectively -10.1232, -9.8082, and -8.5552 kcal/mol. Both madasiatic acid and asiaticoside had common binding residue of Arg189, Glu239, and Glu244.Our study conclude that the active compounds of Centella asiatica (asiaticoside, madecassic acid, and madasiatic acid) had proven to react significantly with SOD and GPx based on docking studies.

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Centella asiatica improved Insomnia through MAPK/ERK Signaling Pathway: In Silico Study

Afif

Santoso

Khotimah

et al. 2023

RJPT

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Insomnia is a difficulty to start sleeping or maintaining the quality of sleep. The latest concept of insomnia explains the disintegration of molecules that change the brain’s rhythm of waking and sleeping. Neurotransmitters that stimulate awakening are orexin, histamine, dopamine, serotonin, while those responsible for sleeping are ɣ-aminobutyric acid (GABA), adenosine, serotonin, melatonin, and prostaglandin D2 (PGD2). Mitogen-Activated Protein Kinase (MAPK)/Extracellular Signal-Regulated Kinase (ERK) pathways have a role in various neuroplasticity that also a candidate mechanism that plays a role in sleep plasticity regulation. Centella Asiatica (CA) is a herb which neuroprotectant effect on neurological diseases. CA can provide anti-inflammatory and rejuvenating effects, antioxidant, anxiolytic, stress relief, and improve intelligence. CA has a role in signaling MAPK/ERK pathway. MAPK3 (ERK1) and MAPK1 (ERK2) proteins are targets that are binded by beta-carotene from CA. In this study, based on QSAR analysis, it proved that CA has benefits for insomnia treatment, as ICAM1 expression inhibitor (0.55), nitric oxide antagonist (0.67), anti-inflammatory (0.80), and gastrin inhibitor (0.45). Therefore, it can be predicted that CA can potentially inhibit ICAM-1 and target AKT1 protein. Then, based on molecular docking, Protein Compound Interaction (PCI) results prove that beta carotene is the most potent MAPK1 inhibitor.

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In vitro Production of Neurologically active Phytoconstituents from an elite Chemovariant of Centella asiatica (L.), identified from south Indian Populations

Cited by 4 publications

References 0 publications

Synthesis and Optimization of Nanoparticle Chitosan-Tripolyphospate Centella asiatica using Ionic Gelation Method with Difference Sonification Time

Synthesis and Optimization of Nanoparticle Chitosan-Tripolyphospate Centella asiatica using Ionic Gelation Method with Difference Sonification Time

In Silico Study of Centella asiatica Derivatives as Antioxidant: Enhancer of Superoxide Dismutase and Glutathione Peroxidase Activity

Centella asiatica improved Insomnia through MAPK/ERK Signaling Pathway: In Silico Study

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