In vitro proliferation of Lebanese Lemna minor and Lemna gibba on different nutrient media

Ghanem, Hassana Hassan; Haddad, Antoine; Baydoun, Safaa; Hamdan, Hassan; Korfali, Samira Ibrahim; Chalak, L.

doi:10.1080/16583655.2019.1597450

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…These values are significantly lower than those reported in the literature for L. minuta and other duckweeds (0.1 ~ 0.5) [ 30 , 31 ]. This may be explained by the relatively long duration of the experiment, as an increase in culture duration may lead to lower doubling time [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the physico-chemical properties of the natural habitat and in vitro culture effects on the biochemistry, proliferation and morphology of Lemna minuta

et al. 2023

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In this study, the ecological conditions of the natural habitat of Lemna minuta Kunth in Morocco were investigated, and the impact of five synthetic growth media (Murashige-Skoog (MS), Schenk-Hildebrand (SH), Hoagland medium (HM), 10X Algal Assay Procedure (AAP), and Swedish Standard Institute medium (SIS)) on the morphophysiological and biochemical parameters was analysed. The morphophysiological parameters included root length, frond surface area, and fresh weight, while the biochemical parameters included photosynthetic pigments, carbohydrates, and protein content. The study was conducted in vitro in two phases: an uncontrolled aeration system (Phase I) and a controlled aeration system (Phase II).The results showed that the pH, conductivity, salinity, and ammonium levels in the natural habitat were within the optimal range for duckweed growth. The measured orthophosphate concentrations were higher compared to previous observations, while the recorded chemical oxygen demand values were low. The study also revealed a significant effect of the culture medium composition on the morphophysiological and biochemical parameters of the duckweed. The fresh weight biomass, relative growth rate in fronds, relative growth rate in surface area, root length, protein content, carbohydrates, chlorophyll (a), chlorophyll (b), total chlorophyll, carotenoids, and the chlorophyll (a/b) ratio were all affected by the culture medium.The most accurate regression models described the growth index GI(F) based on time and in vitro culture conditions in both phases. In Phase I, the best models for MS, SIS, AAP, and SH media were linear, weighted quadratic, cubic, and weighted cubic, respectively. In Phase II, the best models for all growth media were linear. The time coefficients (in days) for Phase II were 0.321, 0.547, 1.232, 1.470, and 0.306 for AAP, HM, MS, SH, and SIS, respectively.Comparing the morphophysiological and biochemical parameters of fronds from different media and analysing the regression model results showed that the SH and MS media were the best among the tested media for the in vitro culture of L. minuta in controlled aeration conditions. However, further research is needed to develop new synthetic media that best promote the growth and maintenance of this duckweed in long-term culture.

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Section: Discussionmentioning

confidence: 99%

Characterization of the physico-chemical properties of the natural habitat and in vitro culture effects on the biochemistry, proliferation and morphology of Lemna minuta

et al. 2023

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“…Fast-growing species show higher RGR under standard cultivation conditions. Higher RGR is due to efficient nutrient and CO 2 uptake in fronds ( Naumann et al., 2007 ; Ziegler et al., 2015 ; Ghanem et al., 2019 ). High RGR causes duckweed to rapidly double its frond number and biomass, which increases its photosynthetically active surface area per unit area.…”

Section: Discussionmentioning

confidence: 99%

“…One of the most important variations is the nutrient medium. Many studies demonstrate that duckweed exhibits optimal growth potential and accumulates more biomass in species-specific nutrient media ( Kittiwongwattana and Vuttipongchaikij, 2013 ; Muranaka et al., 2015 ; Ghanem et al., 2019 ). This study was the first to use a nutrient medium optimized for growth for each species (data in preparation).…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding and biomass accumulation rates of native Iranian duckweed species for biotechnological applications

et al. 2022

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The Lemnaceae family (duckweed) consists of at least three recognized genera with six reported species in Iran that are distributed in wetlands. Duckweeds are the simplest and smallest flowering aquatic monocots with free-floating fronds that can reproduce asexually every 2–3 days. Duckweed could be a major source of balanced amino acids and high protein content, which is increasingly promising for biotechnological applications. For molecular classification and species identification of the collected samples, DNA barcoding was performed using two standard chloroplast markers, the spacer region between the ATP synthase subunits F and H (atpF-atpH) and the intron region of the ribosomal protein S16 (rps16). The results confirm the presence of four species belonging to the two genera Lemna and Spirodela. In addition, L. turionifera was detected for the first time in Iran. Due to the high growth rates of duckweed, measurement of biomass accumulation and doubling time are important factors in determining growth potential, especially for native species. The relative growth rates (RGR), doubling times (DT), biomass accumulation, and relative weekly yields (RY) of 40 distinct duckweed clones were determined under standard cultivation conditions. The dry weight–based RGR ranged from 0.149 to more than 0.600 per day, DT from 1.12 to 9 days, and RY from 7 to 108.9 per week. All values are comparable with previous studies. RGR and RY of selected clones are higher than the growth potential for a wide range of wild plants and common crops. These data support that native duckweed has high productivity value and should be further investigated as a potentially rich protein source for alternative human food, livestock feed, and recombinant protein production.

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“…Duckweed fronds are only a few cells in thickness, and consist of spongy mesophyll with large air spaces that make the fronds buoyant; duckweed are either rootless or bear one to several simple roots on the underside [19]. Under ideal conditions, duckweed plants can double their mass in <2 days, which is faster than for any other higher plant [20].…”

Section: Introductionmentioning

confidence: 99%

Duckweed (Lemna minor L.) Successfully Accumulates Selenium from Selenium-Impacted Water

et al. 2021

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The absorption of selenium (Se) from water by Lemna minor L. and the influence of different concentrations and forms of Se on its biochemical and morphological characteristics were studied. Plants were exposed to various concentrations of Se: 1 mg Se L−1, 2 mg Se L−1 and 5 mg Se L−1 in sodium selenite and sodium selenate solutions and in a combination of selenite (2 mg Se L−1) and selenate (2 mg Se L−1). When the Se was added in the form of selenate, plants accumulated higher amounts of Se compared to plants exposed to selenite. Comparisons of the combined addition of selenite and selenate (2 + 2 mg Se L−1) with their individual applications (2 and 5 mg Se L−1) showed that for the combination, the L. minor fronds accumulated more Se than in selenite alone. Plants exposed to any of the concentrations of sodium selenate or sodium selenite, or the combination of selenite and selenate, showed inferior physiological performances, and lower concentrations of photosynthetic pigments, compared to control plants. Consequently, growth was also suppressed under the stress conditions caused by higher concentrations of Se in any form. The efficient absorption of Se from the water by L. minor indicates the potential use of this species in phytoremediation processes for waters polluted with Se.

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In vitro proliferation of Lebanese Lemna minor and Lemna gibba on different nutrient media

Cited by 4 publications

References 22 publications

Characterization of the physico-chemical properties of the natural habitat and in vitro culture effects on the biochemistry, proliferation and morphology of Lemna minuta

Characterization of the physico-chemical properties of the natural habitat and in vitro culture effects on the biochemistry, proliferation and morphology of Lemna minuta

DNA barcoding and biomass accumulation rates of native Iranian duckweed species for biotechnological applications

Duckweed (Lemna minor L.) Successfully Accumulates Selenium from Selenium-Impacted Water

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