In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value

Wojtania, Agnieszka; Matysiak, B.

doi:10.2478/fhort-2018-0022

Cited by 16 publications

(15 citation statements)

References 43 publications

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“…Sucrose inhibition of shoot formation was previously observed in different plant species, including Helleborus niger L., Paeonia lactiflora Pall , [ 24 , 27 ], Pelargonium hortorum L.H. Bailey [ 28 ] and Rosa ‘Konstancin’ [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation Method for Production of Phenolic-Rich Planting Material of Culinary Rhubarb ‘Malinowy’

Wojtania

Mieszczakowska-Frąc

2021

Plants

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Culinary rhubarb is a popular vegetable crop, valued for its long, thickened stalks, very rich in different natural bioactive ingredients. Tissue cultures are a useful tool for vegetative propagation of virus-free rhubarb plants and rapid multiplication of valuable selected genotypes. The aim of this study was to develop an effective method for in vitro propagation of selected genotypes of Polish rhubarb ‘Malinowy’ characterized by high yield and straight, thick and intensive red stalks. Identification and quantification of anthocyanins and soluble sugars by the HPLC method in shoot cultures and ex vitro established plantlets were also performed. Shoot cultures were established from axillary buds isolated from dormant, eight-year-old rhizomes. Effective shoot multiplication of rhubarb ‘Malinowy’ was obtained in the presence of 6.6 µM benzylaminopurine or 12.4 µM meta-topolin. Both cytokinins stimulated shoot formation in a manner that depended on sucrose concentration. Increasing the sucrose concentration from 59 to 175 mM decreased the production of shoots and outgrowth of leaves by 3-fold but enhanced shoot length, single shoot mass and callus formation at the base of shoots. This coincided with increased accumulation of soluble sugars (fructose, glucose) and anthocyanins-cyanidin-3-O-rutinoside (max. 208.2 mg·100 g−1 DM) and cyanidin-3-O-glucoside (max. 47.7 mg·100 g−1 DM). The highest rooting frequency (94.9%) and further successful ex vitro establishment (100%) were observed for shoots that were earlier rooted in vitro in the presence of 4.9 µM indole-3-butyric acid. Our results indicated that anthocyanin contents in leaf petioles were influenced by developmental stage. Under in vitro conditions, it is possible to elicit those pigments by sucrose at high concentration and meta-topolin.

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Section: Discussionmentioning

confidence: 99%

In Vitro Propagation Method for Production of Phenolic-Rich Planting Material of Culinary Rhubarb ‘Malinowy’

Wojtania

Mieszczakowska-Frąc

2021

Plants

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“…Thus, there is a high demand from the food industry and pharmacy for those fruits, but currently there is no effective vegetative propagation method available for this cultivar. In the previous study, we had developed an efficient in vitro propagation protocol for Rosa 'Konstancin' (Wojtania & Matysiak 2018), which can be used for the production of high-quality stock mother plants.…”

Section: ____________________________________________________________mentioning

confidence: 99%

Vertical Production of ‘Konstancin’ Rose Cuttings in the Growth Chamber Under Led Light

Matysiak

2020

Journal of Horticultural Research

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The impact of light quality generated by light-emitting diodes (LEDs) on the adventitious root formation and cuttings’ quality of rose hip ‘Konstancin’ under controlled conditions in multilevel growth chamber without access to natural light and in the greenhouse was evaluated. In the growth chamber, the lighting was provided by a combination of red and blue LED arrays and white LED tubes, while in the greenhouse, red and blue LED lights were used as a supplement to natural light. The number of rooted cuttings under both growth conditions was not affected by the light conditions generated by LEDs. However, light quality showed significant effects on the biomass production and development of adventitious roots, with the most stimulating red light generated by LEDs. In the growth chamber, the root biomass of the cuttings illuminated with white LEDs supplemented with red LEDs was on average 2.2 times higher than of the cuttings illuminated with white LEDs only. A similar reaction was noted under greenhouse conditions. Supplementation of natural light with red LEDs resulted in 1.7 times increase of the fresh biomass of roots. Both in the growth chamber and in the greenhouse, illumination with red LEDs promoted axillary bud outgrowth. LED lamps can be used in the production of high-quality rose cuttings, both in greenhouses as a complement to natural light and in multilevel rooms as a sole-source lighting for plants.

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“…This method is a viable tool that allows for the rapid and efficient mass production of uniform and pathogen-free plantlets in a short period of time [7]. So far, many studies have been accomplished considering the generation of appropriate micropropagation protocols for several rose species and cultivars [8][9][10]. Regarding R. canina, a number of studies are available on its in vitro regeneration.…”

Section: Introductionmentioning

confidence: 99%

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Samiei

Pahnehkolayi

Tehranifar

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Rosa canina is one of the most popular rose species which is widely used as the rootstock for the propagation of rose cultivars. The purpose of the present study is to improve the in vitro propagation efficiency of this valuable plant species using various growth stimulants in a proliferation medium. In this study, in vitro-derived axillary buds of R. canina were inoculated in Vander Salm (VS) medium supplemented with varying levels of organic or inorganic elicitors including casein hydrolysate (200, 400, and 600 mg/l), glutamic acid (2, 4, 8, and 12 mg/l), proline (500, 1000, 1500, and 2000 mg/l), and silver nitrate (25, 50, 75, and 100 mg/l), separately. Benzyl amino purine (BAP) as well as naphthalin acetic acid (NAA) were added to all media at a constant rate to promote shoot proliferation. Results The results indicated that the supplementation of casein hydrolysate to the VS medium markedly stimulated shoot regeneration by 173% in comparison to control. Shoot proliferation was also positively influenced by glutamic acid at all levels, however, at a lesser extent compared to casein hydrolysate. Silver nitrate at 100 mg/l induced the longest shoots (2.52 ± 0.248 cm) and maximum leaf number (8.90 ± 0.276) among all treatments. Although it did not encourage efficient shoot regeneration, the highest quality shoots with maximum growth vigor were observed in this treatment. Conclusion In this study, the promising role of casein hydrolysate in combination with plant growth regulators has been emphasized for the improved efficiency of R. canina regeneration protocol. Moreover, the addition of silver nitrate to the culture medium seems vital for enhancing the quality of regenerated shoots. The results of this study could be beneficial either for the further pharmaceutical or biochemical investigations of R. canina or commercial purposes for mass propagation of this specimen.

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In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value

Cited by 16 publications

References 43 publications

In Vitro Propagation Method for Production of Phenolic-Rich Planting Material of Culinary Rhubarb ‘Malinowy’

In Vitro Propagation Method for Production of Phenolic-Rich Planting Material of Culinary Rhubarb ‘Malinowy’

Vertical Production of ‘Konstancin’ Rose Cuttings in the Growth Chamber Under Led Light

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

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