<i>In Vitro</i>Secretion of Thyrotropin (TSH): Stimulation by a Hypothalamic Peptide (TRF)

Guillemin, Roger; Yamazaki, Eiichi; Gard, Don A.; Jutisz, M; Sakiz, E

doi:10.1210/endo-73-5-564

Cited by 96 publications

(45 citation statements)

References 8 publications

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“…It has been shown that the pituitary response to TRH is modulated by the level of circulating thyroid hormone (4)(5)(6). However, it is unclear whether thyroxine (T4) alone or triiodothyronine (T3) alone are capable of inhibiting the pituitary response to TRH or whether a combination of both hormones is required.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Pituitary Responsiveness to Throtropin-Releasing Hormone by Triiodothyronine

Shenkman¹,

Mitsuma²,

Hollander³

1973

J. Clin. Invest.

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Section: Introductionmentioning

confidence: 99%

Modulation of Pituitary Responsiveness to Throtropin-Releasing Hormone by Triiodothyronine

Shenkman¹,

Mitsuma²,

Hollander³

1973

J. Clin. Invest.

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“…In fact, it is well established that Ts and T4 administration rapidly decreases plasma TSH value in man [14,17] and consequently causes an inhibition of the thyroid gland function. On the other hand, hypothalamus can also influence TSH secretion: it produces TRH, a polypeptide able to increase TSH secretion from the anterior pituitary gland [3,8,9]. Recent research on laboratory animals has shown that both thyroid hormones and TRH interact at pituitary level [1][2][3][4]; but the mechanism of this interaction is not yet completely clear.…”

mentioning

confidence: 99%

Triiodothyronine and Thyrotropin-Releasing Hormone Interaction on TSH Release in Man

Vigneri¹,

Papalia²,

Pezzino³

et al. 1972

Horm Res Paediatr

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In order to study thyrotropin releasing hormone (TRH)-thyroid hormone interaction, we examined thyrotropin (TSH) behaviour after synthetic TRH administration in 24 euthyroid subjects, 3 patients with primary hypothyroidism and 10 hyper thyroids. 8 normal subjects were studied before and after triiodothyronine (T₃) treatment with increasíng TRH dosage. Our results suggest that (1) TRH-thyroid hormone interaction is dose-related: this means that TSH response to TRH depends on both thyroid hormone and TRH concentration; (2) T₃-TRH interaction on TSH release is only roughly quantitative since it is suggested that other extrathyroidal factors interfere with this mechanism controlling TSH secretion.

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“…This method has been applied successfully in the purification of gonadotropin releasing factor of several species (Guillemin et al, 1963;Schally and Bowers, 1964;Ramirez et al, 1964;Dhariwal et al, 1965 a and b David et al(1965) reported that intracarotid injection of hypothalamic extracts induced a significant decrease of pituitary FSH content of male rats. Recently, Kuroshima et al(1966) have demonstrated that intracarotid injection of beef and pig hypothalamic extracts caused a significant depletion of pituitary FSH content of ovariectomized, estrogen and progesterone-treated rats and a concomitant increase of FSH level in the serum.…”

Section: Discussionmentioning

confidence: 99%

Follicle-Stimulating Hormone-Releasing Factor (Fsh-Rf) and Luteinizing Hormone-Releasing Factor (Lh-Rf) in Rat Hypothalamic Extract

Kobayashi

Kigawa

et al. 1967

Endocrinol Japon

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SYNOPSISHypothalamic tissues of female rats were extracted with 1.M acetic acid. The acidic extract was lyophilized and subjected to gel filtration on Sephadex column and assayed for follicle-stimulating hormone-releasing factor (FSH-RF) activity and luteinizing hormone-releasing factor (LH-RF) activity by estimating FSH and LH using the organ culture method of rat anterior pituitaries. FSH activity was measured by the method of Steelman-Pohley, and LH activity by the method of Parlow. Gel filtration on a short column of Sephadex G-25 in 0.1M pyridine acetate provided a single fraction which showed significant FSH-RF and LH-RF activity. Each activity showed a doserelated increase. Application of a long column of Sephadex G-25 in 0.05M pyridine acetate brought about separation of a peak for FSH-RF activity from that for LH-RF activity. The former activity was eluted from the column just before the appearance of the latter. These results indicated that the secretion of FSH and LH from the rat anterior pituitary was regulated by the specific hypothalamic FSH-RF and LH-RF, respectively.Many experiments have convincingly demonstrated the presence of gonadotropinreleasing factor (GRF) in the rat hypothalamus. Injection of acidic extracts of the hypothalamus induced depletion of ovarian ascorbic acid in immature rats made pseudopregnant by pretreatment with gonadotropin (McCann et al., 1960) and elevated the LH level in plasma of normal or ovariectomized, estrogen and progesterone-treated rats . The extracts also induced ovulation in pentobarbital blocked proestrous rats when infused directly into the pituitary (Nikitovitch-Winer, 1962). We have recently reported that the acidic extracts added to the culture medium stimulate the release of LH by the cultivated pituitaries (Kobayashi et al. 1966). These results indicate the presence of luteinizing hormone-releasing factor (LHRF) in the rat hypothalamus. The existence of follicle-stimulating hormonereleasing factor (FSH-RF) in the rat hypo thalamus has been demonstrated by the fact that injection of the acidic extracts produced a rise in FSH level in plasma of ovariectomized, estrogen and progesterone-treated rats (Igarashi and McCann, 1964) and addition of the extracts stimulated FSH release by the cultivated pituitary (Mittler and Meites, 1964).

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In VitroSecretion of Thyrotropin (TSH): Stimulation by a Hypothalamic Peptide (TRF)

Cited by 96 publications

References 8 publications

Modulation of Pituitary Responsiveness to Throtropin-Releasing Hormone by Triiodothyronine

Modulation of Pituitary Responsiveness to Throtropin-Releasing Hormone by Triiodothyronine

Triiodothyronine and Thyrotropin-Releasing Hormone Interaction on TSH Release in Man

Follicle-Stimulating Hormone-Releasing Factor (Fsh-Rf) and Luteinizing Hormone-Releasing Factor (Lh-Rf) in Rat Hypothalamic Extract

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