In vitroselection of DNA aptamers for the development of chemiluminescence aptasensor for neuron-specific enolase (NSE) detection

Abstract

Neuron-specific enolase (NSE) is one of the most commonly used serum tumor biomarker in clinical practice for small cell lung cancer screening, early diagnosis, evaluation of therapeutic efficacy and prognosis.

Aptamer based surface plasma resonance assay for direct detection of neuron specific enolase and progastrin-releasing peptide (31-98)

“…Though this SPR analysis, the dissociation constant ( K d ) was determined to be about 83 nM, which verifies the relatively high binding affinity of NSE to aptamer. The result is slightly different from that reported in the previous report ( K d ≈ 12 nM), 21 which was measured by using the NSE-immobilized CM5 sensor chip. Through kinetic analysis, the binding rate constant and dissociation rate constant were determined to be 1.21 × 10 4 Ms −1 and 1.004 × 10 −3 s −1 , respectively, which indicate the relatively fast association and slow dissociation of NSE with aptamer.…”

“…A sandwiched chemiluminescence aptasensor for NSE detection in serum samples was developed. 21 By utilizing the signal amplification strategy of enzyme reaction, this aptasensor showed lower detection limit than our direct assay for NSE. Benefitting from the dual signal amplification effect of the synthesized coreshell nanoparticles, a sandwich structured electrochemical sensor showed extremely high sensitivity for NSE detection.…”

“…A sandwiched chemiluminescence aptasensor for NSE detection in serum samples was developed. 21 By utilizing the signal amplication strategy of enzyme reaction, this aptasensor showed lower detection limit than our direct assay for NSE.…”

“…15 Aptamers against various targets, like small molecules, 16 heavy metals, 17 proteins, 18 virus, 19 cells 20 and etc., have been reported and used for constructing biosensors and bioassays. Zheng et al 21 described the rst successful selection of several DNA aptamers with high affinity against NSE, in which the aptamer of Apt-5 showed the highest binding affinity. By using Apt-5, they developed a sandwiched chemiluminescence aptasensor for NSE detection in serum samples.…”

A No‐washing Point‐of‐Care Electrochemical Biosensor Based on CuS Nanoparticles for Rapid and Sensitive Detection of Neuron‐specific Enolase

“…Chemiluminescence Aptasensor 1-100 ng mL À 1 0.1 ng mL À 1 [32] Fluoroimmunoassay 1.25-80 ng mL À 1 0.6 ng mL À 1 [18] Photoelectrochemical immunosensing 0.25-5.0 ng mL À 1 0.08 ng mL À 1 [17] Alternating current impedance 0-0.05 ng mL À 1 0.5 pg mL À 1 [33] Resonance light scattering assay 0.048-150 ng mL À 1 0.015 ng mL À 1 [34] Molecularly imprinted film electrode 0.01-1.0 ng mL À 1 2.6 pg mL À 1 [20] Differential Pulse Voltammetry 0.0005-10.0 ng mL À 1 0.1 pg mL À 1 [35] No-washing Electrochemical biosensor 0.0001-50 ng mL À 1 0.033 pg mL À 1 This method Human IgG (d), (a) + 50 ng mL À 1 AFB1 (e), 1 ng mL À 1 NSE (f), (f) + 50 ng mL À 1 DES (g), (f) + 50 ng mL À 1 CEA (h), (f) + 50 ng mL À 1 Human IgG (i), (f) + 50 ng mL À 1 AFB1 (j). (C) Stability of the biosensor for the detection of 1 ng mL À 1 NSE.…”