In vitro Shoot Cultures of Pterospartum tridentatum as an Alternative to Wild Plants as a Source of Bioactive Compounds

Abstract

The aim of this study was to evaluate the composition of aqueous extracts of in vitro shoot cultures and wild plants of Pterospartum tridentatum in order to promote the use of this plant material as a possible source of bioactive compounds. The extraction yield from wild plants compared with in vitro shoot cultures was lower. The total phenolic contents of in vitro shoots were significantly lower compared with those of wild plants. The phenolic profiles of in vitro shoots were very similar to those of wild plants, regardless of the source. However, taxifolin-6- C-glucoside, as well as rutin and isoquercitrin, were not present in extracts of in vitro shoots. An interesting result was the higher molar percentages of rhamnose and uronic acids detected in in vitro shoots compared with the wild plants, which can make the in vitro plant material very useful for obtaining these compounds.

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

“…In both cases our data are in accordance with the previous reported [14,33,39,40]. The presence of taxifolin glucosides in this species, in particular taxifolin 6-C-glucoside [8,33], was also reported. In this study taxifolin glucosides were found in all extracts, the compounds eluted at 8.59, 8.74, and 9.24 min (Table 1).…”

“…Apparently, this is the first report on their occurrence in this species, however their natural occurrence is well reported and our data are in accordance with the previously reported data, which were also used to establish our assignment [35][36][37][38]. Myricetin 6-C-glucoside (Figure 8), assigned to the compound eluted at 10.89 min, was previously found in the G. tridentata extract [14,33], as well as isorhamnetin 3-O-glucoside (Figure 8), assigned to the compound eluted at 10.89 min. [39].…”

“…Moreover, in this study the flowers were not included, which is the most studied plant part. These aspects explain the differences between the profiles herein discussed and the previously reported ones, in particular the previously reported richness in quercetin derivatives [8,20,33].…”

“…However, in this study attempts to confirm the glucose moieties localization were not performed and we only know that it is a genistein C,O-glucoside derivative. A similar identification could be performed with the derivative eluted at 13.37 min, the occurrence of genistein 8-C-glucoside was previously reported [8,33], however we did not inject standards to confirm the localization of the glucose moiety. Two other isoflavone derivatives identified should be mentioned, the compounds eluted at 9.87 and 11.14 min, which were assigned to formic acid adducts.…”

“…Due to the above-mentioned biological potential of the roots extract and the fact that this plant part was always neglected, indeed the plant aerial parts are the ones used in the medicines Myricetin 6-C-glucoside (Figure 8), assigned to the compound eluted at 10.89 min, was previously found in the G. tridentata extract [14,33], as well as isorhamnetin 3-O-glucoside (Figure 8), assigned to the compound eluted at 10.89 min. [39].…”